Tissue culture technology for stephania erecta

Abstract

The invention discloses a tissue culture technology for stephania erecta. Stephania erecta is selected from dry roots of menispermaceae plants and mainly grows in Yunnan, Guizhou, Guangxi, Sichuan, and the like. Stephania erecta can be harvested throughout the year, optimally in autumn. Stephania erecta is prepared according to the following steps: removing soil and thin fibrous roots, slicing anddrying in the sun. Stephania erecta smells light, tastes bitter, has the functions of clearing away heat and toxic materials, dissolving phlegm, removing stasis and relieving pain, and can be used for treating ulcer pyogenic infection, stomachache, laryngalgia, traumatic injury and venomous snake bites. According to a seedling-growing method for acquiring high-quality seedlings of stephania erecta through an in vitro rapid propagation technology, stephania erecta sections with buds and stems are taken as explants, and then a tissue culture rapid propagation system of stephania erecta is established through the processes of disinfection of explants, induction of multiple shoots, rooting culture, acclimatization and transplanting, and the like. The tissue culture technology has important practical significance in accelerating popularization and resource exploitation and utilization of well-bred stephania erecta.

Description

Technical field [0001] The present invention relates to a method of plant tissue culture in plant biotechnology, and specifically to a tissue culture technology of mountain turtle. Background technique [0002] Mountain turtle is a plant of the family Antipodes, and its dry roots are taken. Mainly produced in Yunnan, Guizhou, Guangxi, Sichuan and other places. It can be harvested all year round, preferably in autumn. Remove soil and fine fibrous roots, slice into slices and dry in the sun. Slight breath and bitter taste. It has the functions of clearing heat, detoxifying, eliminating phlegm, dispersing blood stasis and relieving pain. Used for carbuncle swelling, stomachache, sore throat, bruises, and poisonous snake bites. Mountain turtles have been in short supply for a long time, and man-made predatory logging has led to the depletion of wild mountain turtle resources. Therefore, it is necessary to protect their resources. In recent years, the development of mountai...

AI Technical Summary

Problems solved by technology

At present, the mountain tortoise mainly reproduces seedlings through seeds, but the separation of offspring traits due to seed propagation is not conducive to the protection of high-quality germplasm resources, and restricts the popularization and planting of high-quality mountain tortoises