Separation and purification method for uridine triphosphate

A technology for separation and purification of uridine triphosphate, which is applied in the field of drug purification, can solve problems such as low yield, difficulty in realizing stability and industrialization, and poor purification effect, and achieve stable purification yield, short purification cycle, and high purification efficiency. Yield and stable effect

Active Publication Date: 2019-03-22
SUZHOU NANOMICRO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses two-step ion exchange resins to separate UTP, the purity of the sample after purification is 97%, and the yield is 72.2%, the purification effect is poor, and the yield is relatively low
[0009] Xiao Mingfang and others used the ion exchange method to purify uridine triphosphate in their master thesis "Ion Exchange Method Separation and Purification of 5,-UTP". The purity of the purified sample was 97.17%, and the yield was 92.25%. Most of the resins are self-synthesized in the laboratory, and it is difficult to achieve stability and industrialization

Method used

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  • Separation and purification method for uridine triphosphate
  • Separation and purification method for uridine triphosphate
  • Separation and purification method for uridine triphosphate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Take 47.5 mL of crude uridine triphosphate (65.417% purity, the sample was diluted 5 times with mobile phase A), filter it with a filter membrane with a pore size of 0.45 μm, and collect the filtrate for later use. A 7.7×100mm prepacked column was used, and UniQ-50XS strong anion-exchange microspheres (manufactured by Suzhou Nanowell Technology Co., Ltd.) were used as chromatography column fillers. The column volume was 4.65mL, and the loading capacity was 20g / L.

[0046] Equilibrate the chromatographic column before loading the sample, then load the sample, and then use the gradient elution method for elution.

[0047] The solution of the target peak was collected in sections, and the components meeting the requirements were collected. After high-performance liquid chromatography analysis, the purity of uridine triphosphate in the eluent was 99.05%, and the yield was 90.20%.

[0048] figure 1 It is the scanning electron micrograph of UniQ-50XS strong anion exchange re...

Embodiment 2

[0052] Take 320 mL of crude uridine triphosphate (65.23% purity, the sample is diluted 5 times with mobile phase A), filter it with a filter membrane with a pore size of 0.45 μm, and collect the filtrate for later use. Tricorn 10-200 chromatographic column and UniQ-50XS strong anion-exchange microspheres (produced by Suzhou Nawei Technology Co., Ltd.) were used as the chromatographic column packing with a packing volume of 15.7 mL and a loading capacity of 20 g / L.

[0053] Equilibrate the chromatographic column before loading the sample, then load the sample, and then use the gradient elution method for elution.

[0054] The solution of the target peak was collected in sections, and the components meeting the requirements were collected. After high-performance liquid chromatography analysis, the purity of uridine triphosphate in the eluent was 99.089%, and the yield was 90.24%.

Embodiment 3

[0056] Take 45 mL of crude uridine triphosphate (63.947% purity, the sample was diluted 5 times with mobile phase A), filter it with a filter membrane with a pore size of 0.45 μm, and collect the filtrate for later use. A 7.7×100mm prepacked column was used, and UniQ-50XS strong anion-exchange microspheres (manufactured by Suzhou Nanowell Technology Co., Ltd.) were used as chromatography column fillers. The column volume was 4.65mL, and the loading capacity was 18g / L.

[0057] Equilibrate the chromatographic column before loading the sample, then load the sample, and then use the gradient elution method for elution.

[0058] The solution of the target peak was collected in sections, and the components meeting the requirements were summarized. After high-performance liquid chromatography analysis, the purity of uridine triphosphate in the eluent was 99.408%, and the yield was 86.54%.

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Abstract

The invention provides a separation and purification method for uridine triphosphate. The separation and purification method comprises the following steps: 1) loading a sample: filtering a crude product of uridine triphosphate and then loading into a chromatographic column, wherein chromatographic column filler is made of monodisperse microspheres taking polyacrylate as substrate; 2) eluting: using an eluant for eluting UTP absorbed in the chromatographic column; 3) collecting and gathering: fractionally collecting eluted uridine triphosphate solution and gathering the component liquid meetingthe requirement for target peak. The separation and purification method for uridine triphosphate provided by the invention is simple and convenient and is short in purification period; the requirement for high purity of uridine triphosphate can be met by only one-step chromatographic purification; purity can reach up to 99% or above; purification yield is high and stable.

Description

technical field [0001] The invention relates to the technical field of medicine purification, in particular to a method for separating and purifying uridine triphosphate. Background technique [0002] Uridine triphosphate (UTP for short) is a uracil nucleotide, which is composed of a uracil, a ribose sugar, and three phosphates linked together, in which the phosphate group is linked to the 5' carbon. UTP is closely related to carbohydrate metabolism, UDP-glucose and pyrophosphate are generated from UTP and 1-phosphate glucose by enzymatic catalysis. In addition, UDP-galactose, UDP-galactosamine, UDP-glucuronic acid, etc. are also produced. [0003] UTP participates in the synthesis of pyrimidine ribonucleotides and is the raw material for RNA synthesis (transcription). In addition, UTP can also be used as an energy source, and its function is similar to that of ATP, but it is less common than ATP. UTP is also involved in many energy metabolism pathways in organisms. [00...

Claims

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Application Information

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IPC IPC(8): C07H19/10C07H1/06
CPCC07H1/06C07H19/10
Inventor 江必旺石凌超邹胜张彦辉
Owner SUZHOU NANOMICRO TECH CO LTD
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