Method for determining nitroimidazole drug residues in blood sample

Abstract

The invention discloses a method for determining nitroimidazole drug residues in a blood sample. The method is characterized by comprising the following steps: (1) preparing a supermolecular solvent:n-octyl alcohol, tetrahydrofuran and water are adopted, stirred and centrifuged, and an upper organic phase is taken; (2) pretreating the sample: the blood sample is placed in the supermolecular solvent, vibrating extraction and centrifugation are performed, an upper liquid filtering membrane is taken, a filtrate is placed into a micro-sampling bottle, methanol is added, and a to-be-tested solution is obtained by uniform mixing; (3) determining the nitroimidazole drug residues in the to-be-tested solution with ultra-performance liquid chromatography-tandem mass spectrometry. The method is simple, fast, accurate and reliable and can be used for analyzing and detecting the nitroimidazole drug residues in the blood sample.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for determining nitroimidazole drug residues in blood samples. Background technique [0002] Nitroimidazoles are a class of compounds with a 5-nitro-substituted imidazole heterocyclic structure. Because of their unique antibacterial and antiprotozoal effects, they are widely used to kill and prevent anaerobic bacteria and pathogens. Common nitroimidazoles mainly include metronidazole, dimetridazole, pronidazole, and ronidazole. Nitroimidazole drugs are potentially carcinogenic, teratogenic and mutagenic, and their metabolites have similar toxicity to the original drug because they also contain an imidazole ring. Therefore, China, the European Union, the United States and other countries and regions prohibit the use of animal-derived foods use of these drugs. At present, the detection methods of nitroimidazole drugs mainly include gas chromatography, gas chromatography-mass spectro...

AI Technical Summary

Problems solved by technology

Among them, the protein precipitation method is simple to operate, but the removal effect on proteins with smaller molecular weight and most of the phospholipids is poor; the liquid-liquid extraction method has a better purification effect on proteins, but the co-extraction efficiency of organic solvents on phospholipids is very high, and The operation is cumbersome and time-consuming; the purification effect of solid-phase extraction on blood samples is weak, which may cause the co-extraction effect of the target object and protein or phospholipid, and the operation is complicated and costly

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