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A Mutagenic Strain of Monascus Violet and a Water-Soluble Functional Monascus and Its Preparation Method and Application

A purple Monascus, water-soluble technology, applied in the biological field, can solve the problems of difficult intracellular and extracellular metabolite secretion, serious bitterness of solid-state fermentation products, difficult products, etc., and achieves increased application scope, easy large-scale production, and good water solubility. Effect

Active Publication Date: 2020-11-27
GUANGDONG TIANYI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitation of solid-state fermentation conditions, it is difficult to regulate the secretion of extracellular and extracellular metabolites in the solid-state fermentation of the triangular flask. The production of red yeast rice with high ring-opening function has not been reported, and the solid-state fermentation product has a severe bitter taste and is difficult to dissolve in water.
The liquid fermentation of glycerin, because glycerin is a chemical raw material, GB2760-2014 "National Food Safety Standard Food Additive Use Standard" strictly restricts the amount of glycerin used as a food additive raw material, using glycerin as a fermentation raw material for large-scale fermentation to produce functional red Qu has not been reported yet

Method used

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  • A Mutagenic Strain of Monascus Violet and a Water-Soluble Functional Monascus and Its Preparation Method and Application
  • A Mutagenic Strain of Monascus Violet and a Water-Soluble Functional Monascus and Its Preparation Method and Application
  • A Mutagenic Strain of Monascus Violet and a Water-Soluble Functional Monascus and Its Preparation Method and Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] (1) Preparation of seed solution

[0066] Preparation of primary seed solution:

[0067] The mutant strain of Monascus purpureus Went was used as the production seed. Inoculate the sterilized and cooled seed medium (glucose 5%, peptone 2.0%, corn steep liquor 0.5%, NaNO 3 0.1 %, MgSO4•7H 2 O 0.05%, K 2 HPO 4 •3H 2 O 0.05%, the components are calculated by weight to volume ratio, and the unit is kg / m 3 ; pH value (natural), 30°C, 150 rpm, ventilation ratio 1:1.5, tank pressure 0.04Mpa, culture for 36 hours, so that the bacteria are in the logarithmic growth phase.

[0068] Preparation of secondary seed solution:

[0069] Inoculate the sterilized and cooled seed medium (glucose 6%, peptone 2.5%, corn steep liquor 0.8%, NaNO 3 0.12%, MgSO 4 •7H 2 O 0.05%, K 2 HPO 4 •3H 2 O 0.05%, the components are calculated by weight to volume ratio, and the unit is kg / m 3 ; pH value (natural), 30°C, 120rpm, ventilation ratio 1:1.6, tank pressure 0.04Mpa, culture for 28h,...

Embodiment 2

[0074] (1) Preparation of seed solution

[0075] Preparation of primary seed solution:

[0076] The mutant strain of Monascus purpureus Went was used as the production seed. Inoculate the sterilized and cooled seed medium (glucose 6%, peptone 2.5%, corn steep liquor 0.8%, NaNO 3 0.15 %, MgSO4•7H 2 O 0.08%, K 2 HPO 4 •3H 2O 0.08%, the components are calculated by weight to volume ratio, and the unit is kg / m 3 ; pH value (natural), 31 ° C, 180 rpm, ventilation ratio 1:1.6, tank pressure 0.04Mpa, culture for 32 hours, so that the bacteria are in the logarithmic growth phase.

[0077] Preparation of secondary seed solution:

[0078] Inoculate the sterilized and cooled seed medium (glucose 6.5%, peptone 2.8%, corn steep liquor 1.0%, NaNO 3 0.2%, MgSO 4 •7H 2 O 0.08%, K 2 HPO 4 •3H 2 O 0.08%, the components are calculated by weight to volume ratio, and the unit is kg / m 3 ; pH value (natural), 31°C, 150rpm, ventilation ratio 1:1.8, tank pressure 0.04Mpa, culture for 2...

Embodiment 3

[0083] (1) Preparation of seed solution

[0084] Preparation of primary seed solution:

[0085] The mutant strain of Monascus purpureus Went was used as the production seed. Inoculate the sterilized and cooled seed medium (glucose 8%, peptone 2.8%, corn steep liquor 1.0%, NaNO 3 0.2%, MgSO4•7H 2 O 0.1%, K 2 HPO 4 •3H 2 O 0.1%, the components are calculated by weight to volume ratio, and the unit is kg / m 3 ; pH value (natural), 32°C, 200 rpm, ventilation ratio 1:1.8, tank pressure 0.05Mpa, culture for 24 hours, so that the bacteria are in the logarithmic growth phase.

[0086] Preparation of secondary seed solution:

[0087] The primary seed solution in the logarithmic growth phase was inoculated at 10% inoculum volume (V / V) in the sterilized and cooled seed medium (glucose 8.5%, peptone 3.0%, corn steep liquor 1.2%, NaNO 3 0.25%, MgSO 4 •7H 2 O 0.12%, K 2 HPO 4 •3H 2 O 0.12%, the components are calculated by weight to volume ratio, and the unit is kg / m 3 ; pH v...

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Abstract

The invention discloses a purple Monascus mutagenic strain, a water-soluble functional red yeast rice and its preparation method and application. The purple Monascus mutagenic strain was preserved in the China Type Culture Collection Center on September 3, 2018 , deposit number: CCTCC NO: M2018586. The Monascus purple mutagenic strain can be used to produce red yeast rice with high ring-opening function, and hardly produces citrinin. In addition, the present invention puts the Monascus purple mutagen strain in a special fermentation medium for liquid submerged fermentation, efficiently synthesizes monacolin K with a high ring-opening structure, and then obtains water-soluble functional red yeast rice through a post-treatment process. It has the characteristics of high ring-opening acid structure as the main body, good water solubility, good flavor, no citrinin or very low content. Moreover, the fermentation raw material is a non-glycerin raw material, and there is no potential safety hazard of glycerin residue. The preparation method provided by the invention has simple process, is easy for large-scale production, is environmentally friendly, and the prepared product meets the safety and quality requirements of food, health care products and medicines.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, to a mutagenic strain of Monascus purple, a water-soluble functional red yeast rice, a preparation method and application thereof. Background technique [0002] Red yeast rice has the effects of invigorating the spleen and stomach, promoting blood circulation, removing blood stasis and relieving pain. It has a long history of edible and medicinal use in my country. In 1979, Endo et al. found that the Monacolin series of substances contained in the red yeast rice of a special Monascus can inhibit the HMG-CoA reductase (HMG-CoA reductase) in the biosynthesis of cholesterol in the human body. The role of this kind of red yeast rice is called lipid-lowering red yeast rice, also known as functional red yeast rice. [0003] With the gradual improvement of people's living standards and the increasingly obvious problem of aging population, the incidence of hyperlipidemia is increasing yea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A23L33/00A61K36/06A61P3/06C12R1/645
CPCA23V2002/00A61K36/06A23L33/00A61P3/06C12N1/145C12R2001/645A23V2200/3262
Inventor 许世锦胡文林陈映妮聂增宇陈玲娟陈晓锋
Owner GUANGDONG TIANYI BIOTECH CO LTD