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Biocontrol preparation for disease prevention of tobacco and preparation method thereof

A technology of biocontrol agents and tobacco, which is applied in the direction of botany equipment and methods, biocides, biocides, etc., can solve the problems of affecting, unfavorable growth and function of useful microorganisms, and difficulty in controlling the number and variety of microorganisms, so as to save production Cost, the effect of saving biological resources

Inactive Publication Date: 2019-03-29
LINCANG OF YUNNAN TOBACCO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many microbial agents are used to regulate the soil microbial environment of crops to reduce the growth of pathogenic microorganisms and promote crop growth. However, producers need to ensure that the microorganisms in the agent are alive and can Survive and reproduce in the soil environment. In actual production, if several different microorganisms are added to the crop soil at the same time, there will be a competitive survival relationship between them, which will inevitably lead to the growth advantage of certain microorganisms, which is not conducive to the growth and development of other useful microorganisms. Play a role; when the soil microbial environment of the original crops is unknown, the addition of foreign microorganisms will have a certain impact on the original soil microbial environment, and the number and variety of microorganisms are difficult to control

Method used

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  • Biocontrol preparation for disease prevention of tobacco and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0022] The preparation of embodiment one tobacco biocontrol preparation

[0023] 1 source of material

[0024] Bacillus subtilis, Trichoderma harzianum, and streptomycin were purchased from commercial sources.

[0025] Plant ash is the ashes left after plant fuels are burned for power generation.

[0026] Sugarcane powder comes from sugarcane waste residue after the sugar mill has squeezed the juice, which is crushed into powder.

[0027] 2 microbial culture

[0028] 2.1 Bacillus subtilis

[0029] (1) Medium formula

[0030] Seed medium: peptone 1%, yeast extract 0.5%, sodium chloride 1%, natural pH, sterilized;

[0031] Basic fermentation medium: 1% sucrose, 1% peptone, 0.2% disodium hydrogen phosphate, 0.2% sodium dihydrogen phosphate, pH 7.0, sterilized.

[0032] (2) Culture method

[0033] A. Activation of bacteria

[0034] In a sterile environment, transfer the purchased strains to the slant medium, culture at 37°C for 24 hours, and set aside.

[0035] B. Prepara...

Embodiment 2

[0060] Application of Example 2 Biocontrol Agents in Tobacco Planting

[0061] Use the compound microbial agent produced by the method of embodiment 1 to carry out field test, test site: Chahe Township, Eshan County, Yunnan Province, the test species is flue-cured tobacco, and the experimental field for testing is a plot with a serious incidence rate in recent years, and after organic Fertilizer spread. The test is provided with 2 treatments, which are respectively: 1: do not use the biocontrol preparation, as a control group; 2: use the biocontrol preparation powder of the present invention to spread, and the application rate is 5kg / mu, and each treatment is repeated 4 times. 100m 2 . Observe the disease on 60 days after planting. Disease grade: 0 is asymptomatic on the leaves; 1 is wilting on less than 1 / 4 of the leaves on the plant; 2 is wilting on 1 / 4 to 1 / 2 of the leaves; 3 Grade 1 means that more than 1 / 2 of the leaves of the plants show wilting symptoms; Grade 4 mean...

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Abstract

The invention relates to the technical field of plant biocontrol preparations, specifically to a biocontrol preparation for disease prevention of tobacco and a preparation method thereof. The invention aims to provide a biocontrol preparation for disease prevention of tobacco and a preparation method thereof. The preparation method comprises the following steps: evenly mixing a mixed medium freeze-dried powder, sugarcane powder and plant ash according to a certain ratio so as to obtain pulvis of the biocontrol preparation for disease prevention of tobacco; mixing the mixed medium freeze-driedpowder and some organic matter and dissolving by adding water so as to obtain the biocontrol preparation for disease prevention of tobacco. The mixed medium freeze-dried powder comprises medium freeze-dried powder of bacillus subtilis, Trichoderma harzianum and Streptomyces microflavus. The biocontrol preparation for disease prevention of tobacco can control some common but hazardous diseases of tobacco.

Description

technical field [0001] The invention relates to the technical field of plant biocontrol preparations, in particular to a tobacco disease prevention and biocontrol preparation and a preparation method thereof. Background technique [0002] Tobacco is a crop with high economic value. my country has a large area of ​​tobacco planting and a long history of planting. However, the problems of tobacco pests and diseases during the planting process and the pollution of the environment by traditional chemical fungicides and insecticides are becoming more and more serious. Root rot, vein spot, bacterial wilt, and black shank are infectious diseases with high incidence and serious damage to tobacco crops. The transmission method is mainly soil transmission, and traditional chemical control methods are difficult to obtain obvious effects. preventive effect, and is likely to cause environmental pollution. Under the background of the country's sustainable development strategy of pollutio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N63/04A01P1/00A01P3/00
CPCA01N63/10A01N63/30
Inventor 何元胜曹敬东吴剑许银莲王继明郑元仙魏佳刘佳妮钟宇魏薇余磊
Owner LINCANG OF YUNNAN TOBACCO
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