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Application of exosome from endometrium stem cells to medicines for treating acute lung injury

A technology of endometrial stem cells and acute lung injury, which is applied in the field of acute lung injury treatment, can solve problems such as undiscovered treatment methods, achieve the effect of improving blood oxygen saturation and oxygenation index, and improving the concentration of inflammatory factors in the lungs

Active Publication Date: 2019-03-29
HANGZHOU S EVANS BIOSCI LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a large number of studies have attempted to elucidate the pathogenesis of ALI, no particularly effective treatment has been found so far.

Method used

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  • Application of exosome from endometrium stem cells to medicines for treating acute lung injury
  • Application of exosome from endometrium stem cells to medicines for treating acute lung injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Preparation of acute lung injury mouse model

[0016] Mice were injected with 250 μl of 1% sodium pentobarbital (prepared in PBS) for anesthesia, cut the skin and muscle layer along the middle of the neck, exposed the trachea, and inserted 2.5mg / ml of LPS (prepared in PBS) at a dose of 10 mg / kg body weight. Immediately after the injection, the mice were turned upright so that the solution was evenly distributed on both sides of the lungs. After suturing, the mice were fed normally in a sterile environment.

Embodiment 2

[0017] Example 2: Endometrial stem cell exosome preparation

[0018] (1) Isolation and culture of endometrial stem cells

[0019] Take 20ml of menstrual blood, centrifuge at 1000rpm at room temperature for 3 minutes, discard the supernatant, add 10ml of normal saline to fully mix the cell pellet. Slowly transfer to a centrifuge tube containing 15ml of human lymphocyte separation medium, and centrifuge at 600g for 20min at room temperature. Carefully pipette the middle buffy coat (monocyte layer) into another centrifuge tube. Add an appropriate amount of saline and mix thoroughly, centrifuge at 1000rpm for 5min, and discard the supernatant to obtain the cell pellet. Suspend the obtained precipitate with MEM-alpha medium (purchased from Invitrogen) containing 2% serum substitute (purchased from PALL), and take a small amount of suspension to count through an automatic blood analyzer, and finally nucleated cells After adjusting the density to 2*10 5 / cm 2 Inoculation density...

Embodiment 3

[0022] Example 3: Infusion of endometrial stem cell exosome preparation improves lung function and reduces inflammation of lung injury

[0023] (1) Utilize the mouse acute lung injury (ALI) model that the method described in Example 1 establishes, experiment is 3 groups: group 1 is the ALI model control group, group 2 is the ALI model PBS injection group, group 3 is the ALI model palace Endometrial stem cell exosome preparation injection group. Group 3 was infused with the endometrial stem cell exosome preparation prepared in Example 2 through the tail vein at a dose of 1 mg / kg body weight 6-24 hours after modeling. Group 2 was injected with the same volume of PBS as Group 3. Samples were taken 72 hours after injection for blood gas analysis and inflammatory factor detection to analyze the effect of endometrial stem cell exosomes on ALI.

[0024] (2) Blood gas analysis and detection: After the mice were anesthetized by injecting 250 μl of 1% pentobarbital sodium (prepared in...

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Abstract

The invention discloses an application of exosome from endometrium stem cells to medicines for treating acute lung injury. The endometrium stem cells are from menstrual bleeding of women. According tothe application disclosed by the invention, the endometrium stem cells are convenient to obtain and rich in source, an endometrium stem cell exosome preparation is simple to prepare, and an infusiontechnique is simple, convenient and easy to operate; and the exosome has significant effects on improving lung functions and reducing inflammation during acute lung injury, and has important clinicalsignificance and great application prospects.

Description

technical field [0001] The invention relates to the field of acute lung injury treatment, in particular to the use of endometrial stem cell-derived exosomes in the treatment of acute lung injury and a preparation method thereof. Background technique [0002] Acute lung injury (ALI) is mainly caused by gastric acid inhalation, infection, trauma, fat embolism, inhalation of toxic gas and other reasons, and inflammation plays a major role in its pathological changes. Although a large number of studies have attempted to elucidate the pathogenesis of ALI, no particularly effective treatment has been found so far. At present, the treatment of ALI is mainly based on supportive treatment, drug therapy and assisted ventilation therapy, and the clinical mortality rate is still very high, which is maintained at about 30% to 40%. Therefore, it is urgent to develop effective drugs and methods for ALI treatment. . [0003] In recent years, studies have shown that there are abundant plur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/48A61P11/00
CPCA61P11/00A61K35/48
Inventor 潘若浪张强吴赟婷戴玲华王金福
Owner HANGZHOU S EVANS BIOSCI LTD
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