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A double-network-loaded chitosanase hydrogel dressing based on three-dimensional printing technology and its preparation method

A chitosan-loaded, three-dimensional printing technology, applied in bandages, additive processing, medical science, etc., can solve the problems of difficult mechanism, complicated reaction process, high cost, etc., and achieves favorable adhesion and reproduction, and simple preparation method , good functional effect

Active Publication Date: 2022-01-14
武汉知音堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that adding biological enzymes to chitosan-based dressings can not only improve the antibacterial properties of dressings, but also help to improve the mechanical properties and stability of dressings. High, and the mechanism is not easy to grasp, so the mechanism of studying the dressings of chitosan and its derivatives loaded with chitosanase is clearer, easier to grasp, and more conducive to the preparation of chitosan-based dressings with stable functions

Method used

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  • A double-network-loaded chitosanase hydrogel dressing based on three-dimensional printing technology and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Add free chitosan enzyme solution to gelatin methacryloyl aqueous solution, add photocrosslinking agent, stir and crosslink, freeze at -20°C for 4 hours, take it out, dissolve completely at room temperature, and obtain loaded chitosan Enzyme gelatin methacryloyl aqueous solution, wherein the activity value of chitosanase in the gelatin methacryloyl aqueous solution loaded with chitosanase is 24U / mL.

[0030] (2) According to the dosage ratio of chitosan, polyethylene glycol, paraffin and formaldehyde is 1g:5mL:20mL:15mL, dissolve chitosan in the acidic solution, stir well, add polyethylene glycol, paraffin and formaldehyde , after microwave pretreatment at 500W power for 10s, at room temperature, high-speed stirring at 8000r / min for 1h, filtered and washed to obtain chitosan-modified polyethylene glycol aqueous solution.

[0031](3) the polyethylene glycol aqueous solution of gelatin methacryloyl aqueous solution and the chitosan modification that the volume ratio i...

Embodiment 2

[0034] (1) Add free chitosan enzyme solution to gelatin methacryloyl aqueous solution, add photocrosslinking agent, stir and crosslink, freeze at -20°C for 6h, take it out, dissolve completely at room temperature, and obtain loaded chitosan Enzyme gelatin methacryloyl aqueous solution, wherein the activity value of chitosanase in the gelatin methacryloyl aqueous solution loaded with chitosanase is 26U / mL.

[0035] (2) According to the dosage ratio of chitosan, polyethylene glycol, paraffin and formaldehyde is 1g:8mL:40mL:20mL, dissolve chitosan in the acidic solution, stir well, add polyethylene glycol, paraffin and formaldehyde , after microwave pretreatment at 800W power for 90s, at room temperature, high-speed stirring at 14000r / min for 2h, filtered and washed to obtain chitosan-modified polyethylene glycol aqueous solution.

[0036] (3) the polyethylene glycol aqueous solution of gelatin methacryloyl aqueous solution and the chitosan modification that the volume ratio is t...

Embodiment 3

[0039] (1) Add free chitosan enzyme solution to gelatin methacryloyl aqueous solution, add photocrosslinking agent, stir and crosslink, freeze at -20°C for 5h, take it out, dissolve completely at room temperature, and obtain loaded chitosan A gelatin methacryloyl aqueous solution of enzyme, wherein, the activity value of chitosanase in the gelatin methacryloyl aqueous solution loaded with chitosan enzyme is 25 U / mL.

[0040] (2) According to the dosage ratio of chitosan, polyethylene glycol, paraffin and formaldehyde is 1g7mL: 30mL: 17mL, chitosan is dissolved in the acidic solution, stir well, add polyethylene glycol, paraffin and formaldehyde, in After microwave pretreatment at 600W power for 30s, at room temperature, high-speed stirring at 11000r / min for 1.5h, filtered and washed to obtain chitosan-modified polyethylene glycol aqueous solution.

[0041] (3) the aqueous gelatin methacryloyl aqueous solution of the load chitosanase of 1:0.7 and the polyethylene glycol aqueous...

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Abstract

The invention provides a double-network-loaded chitosanase hydrogel dressing based on three-dimensional printing technology and a preparation method thereof, comprising the following steps: adding free chitosanase solution to gelatin methacryloyl aqueous solution, adding light crosslinking Linking agent, stirring and cross-linking, freeze-thawing treatment, to obtain gelatin methacryloyl aqueous solution loaded with chitosanase; dissolving chitosan in acidic solution, stirring evenly, adding polyethylene glycol, paraffin and formaldehyde, microwave After the pretreatment, stir at room temperature at high speed, filter and wash to obtain the polyethylene glycol aqueous solution modified by chitosan; the gelatin methacryloyl aqueous solution loaded with chitosanase and the chitosan modified prepared by step (2) The polyethylene glycol aqueous solution is stirred and mixed evenly to form a two-phase emulsion; the two-phase emulsion is frozen at a low temperature to form a hydrogel state, and a three-dimensional network structure is formed by 3D printing, and then the polyethylene glycol is removed by heating to obtain a three-dimensional printing technology. A double network loaded chitosanase hydrogel dressing.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a double-network-loaded chitosanase hydrogel dressing based on three-dimensional printing technology and a preparation method thereof. Background technique [0002] Chitosan is a chitin derivative that removes about 70% of the acetyl groups from nail chitin after concentrated alkali and high temperature treatment. Chitosan is also known as polyacetylglucosamine. The molecule of chitosan is a linear structure, which contains a large number of electronegative groups such as antelope, amino, and acetamido groups. Therefore, it is easy to form strong hydrogen bonds in or between macromolecules of chitosan, making chitosan Sugar macromolecules have good regularity and crystallinity, and are soluble in various dilute acids, and chitosan macromolecules have a structure similar to plant cellulose and human collagen tissue, so chitosan Sugar has good biocompatibilit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00B33Y70/00
CPCA61L26/0023A61L26/0038A61L26/0047A61L26/008B33Y70/00A61L2300/232A61L2300/404C08L89/00C08L71/02
Inventor 方旭波陈小娥余辉孙海燕
Owner 武汉知音堂生物科技有限公司