Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescence detection test paper and its preparation method and application, identification method of inferior oil

A fluorescence detection and test paper technology, applied in the field of food chemistry, can solve the problems of poor specificity, low sensitivity and accuracy, slow detection speed, etc. Accurate effect

Active Publication Date: 2022-03-25
清水湾生物材料(深圳)有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, provide a fluorescent detection test paper and its preparation method and application, and a method for identifying inferior edible oils, aiming at solving the problem of poor specificity, sensitivity and accuracy of existing inferior oil identification Not high, and the technical problems of slow detection speed and complex equipment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescence detection test paper and its preparation method and application, identification method of inferior oil
  • Fluorescence detection test paper and its preparation method and application, identification method of inferior oil
  • Fluorescence detection test paper and its preparation method and application, identification method of inferior oil

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] On the other hand, the embodiment of the present invention also provides a preparation method of the above-mentioned fluorescence detection test paper. The preparation method comprises the following steps:

[0033] S011: Provide a test paper substrate, and prepare a probe solution containing fluorescent molecular probes;

[0034] S012: Using at least one of chemical coupling method, physical adsorption method, spraying method and electrostatic adsorption method, the fluorescent molecular probe in the probe solution is combined into the porous structure of the test paper substrate, and then dried to obtain fluorescence detection test paper.

[0035] The preparation method of the fluorescent detection test paper utilizes the test paper base material and the polarity-sensitive fluorescent probe to uniformly bind the polarity-sensitive fluorescent probe to the base material; the process is simple and easy to operate, the conditions are easy to control, the production cost ...

Embodiment 1

[0049] A fluorescence detection test paper, comprising: a polycarbonate membrane (pore size range: 1 nm to 100 nm) and 9-(diethylamino)-5H-benzo[A]phene combined in the porous structure of the polycarbonate membrane Oxazin-5-one fluorescent probe. Its preparation method comprises the following steps:

[0050] S111: Provide polycarbonate film and 9-(diethylamino)-5H-benzo[A]phenoxazin-5-one raw material, 9-(diethylamino)-5H-benzo[A] The phenoxazin-5-one raw material was dissolved in pure ethanol to obtain 0.2 mg / mL 9-(diethylamino)-5H-benzo[A]phenoxazin-5-one ethanol solution.

[0051] S112: After soaking the above polycarbonate film in 9-(diethylamino)-5H-benzo[A]phenoxazin-5-one ethanol solution for 1 hour, it is dried with an air gun to obtain a fluorescence detection test paper.

[0052] In order to further improve the adsorption capacity of the test paper surface for total polar substances in this example, the polycarbonate membrane was placed in 9-(diethylamino)-5H-benz...

Embodiment 2

[0054] Different types of edible oils are used to detect fluorescence intensity with the detection test paper of Example 1, and the specific process is as follows:

[0055]Taking sunflower oil, rapeseed oil and corn oil as three fresh edible oils as standard samples, and taking water, ethanol and glycerol as control, waste oil (obtained in Shenzhen market) and expired sunflower oil were also provided. In addition, the following frying oil sample oil was obtained: add 1.8L sunflower oil to the frying pan, fry 60g freshly frozen potato chips in 180-185 ℃ sunflower oil for 5 minutes as a cycle, take out the fried pieces and wait Allow the oil to cool for 25 minutes before starting to repeat the next cooking cycle. After every 5 cycles, the fried oil was cooled to room temperature, and then a small amount of oil was removed as a test sample (about 10 mL), which was filtered through a 0.45 μm pore syringe filter (Millipore™) to remove food suspensions. In this way, oil for 5 cycle...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of food chemistry, in particular to a fluorescent detection test paper, which comprises: a test paper base material and a fluorescent molecular probe combined in the test paper base material; the fluorescent molecular probe is polar Sensitive fluorescent probe capable of identifying trace polar impurities in non-polar solutions. The method for identifying inferior oils and fats provided by the present invention utilizes the fluorescence detection test paper of the present invention to detect changes in the fluorescence spectra of standard oils and fats and samples to be tested. It can detect the quality of grease or identify inferior grease (such as waste oil) within a few seconds. It has the characteristics of high sensitivity, strong specificity, high accuracy, fast detection speed and low cost.

Description

technical field [0001] The invention belongs to the technical field of food chemistry, and in particular relates to a fluorescence detection test paper, a preparation method and application thereof, and a method for identifying inferior oils and fats. Background technique [0002] Edible oil, also known as "edible oil", refers to the animal or vegetable oil used in the process of making food. It is liquid at room temperature. Due to the source of raw materials, processing technology and quality, common edible oils are mostly vegetable oil. After the cooking oil is heated at high temperature or repeatedly processed, some harmful substances are produced due to peroxidation or decomposition. Therefore, the cooking oil after frying or cooking for a period of time should be discarded. to decide, lacking a reliable standard. However, some unscrupulous businessmen, driven by profit, repeatedly recycled the edible oil used in restaurants, and after being refined in underground work...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439
Inventor 曹文彬
Owner 清水湾生物材料(深圳)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com