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Application of ophiocordyceps sinensis to preparation of medicines for preventing or treating pulmonary hypertension

A technology of pulmonary arterial hypertension and cordycepic acid, which can be used in drug combinations, active ingredients of hydroxyl compounds, respiratory system diseases, etc., can solve problems such as Cordyceps sinensis that have not yet been found, and achieve the effects of alleviating symptoms, reducing apoptosis, and promoting proliferation.

Inactive Publication Date: 2019-04-02
雷桅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, no reports of Cordyceps sinensis and its active ingredient cordycepic acid being used to prepare drugs for the treatment of pulmonary arterial hypertension have been found.

Method used

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  • Application of ophiocordyceps sinensis to preparation of medicines for preventing or treating pulmonary hypertension
  • Application of ophiocordyceps sinensis to preparation of medicines for preventing or treating pulmonary hypertension
  • Application of ophiocordyceps sinensis to preparation of medicines for preventing or treating pulmonary hypertension

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Interventional effect of cordycepic acid on the activity of pulmonary artery endothelial cells

[0026] 1. Experimental materials

[0027] Experimental subjects: Human pulmonary artery endothelial cells (HPAECs) were purchased from Science Cell Company. The sixth-generation HPAECs in logarithmic growth phase were used. They were inoculated after trypsin digestion. When the cells grew to about 70%, serum-free DMEM was used. Low-glucose medium was starved for 18 h, then normoxia, hypoxia, hypoxia + drug intervention respectively.

[0028] Main reagents: Cordyceps acid, fetal bovine serum, PBS (phosphate buffered solution), trypsin, DMEM low glucose medium, ECM complete medium, CCK-8 kit.

[0029] Main instruments: cell culture dish, cell incubator, 96-well plate, microscope, biological safety cabinet, alcohol lamp, Pasteur tube, 15ml / 50ml centrifuge tube, pipette, alcohol cotton ball, microplate reader.

[0030] 2. Experimental method

[0031] HPAEC cells in ...

Embodiment 2

[0036] Example 2 Detection of apoptosis by Annexin V-FITC / PI double staining method

[0037] 1. Experimental materials

[0038] Experimental subjects: Human pulmonary artery endothelial cells (HPAECs) were purchased from Science Cell Company. The sixth-generation HPAECs in logarithmic growth phase were used. They were inoculated after trypsin digestion. When the cells grew to about 70%, serum-free DMEM was used. Low-glucose medium was starved for 18 h, then normoxia, hypoxia, hypoxia + drug intervention respectively.

[0039] Main reagents: Cordyceps acid, fetal bovine serum, PBS, trypsin, DMEM low glucose medium, ECM complete medium, Annexin V kit.

[0040]Main instruments: cell culture dish, cell incubator, 96-well plate, microscope, biological safety cabinet, alcohol lamp, Pasteur tube, 15ml / 50ml centrifuge tube, pipette, alcohol cotton ball, microplate reader.

[0041] 2. Experimental method

[0042] Inoculate HPAEC cells in logarithmic growth phase with ECM complete me...

Embodiment 3

[0045] Example 3 TUNEL method detects the effect of cordycepin on cell damage

[0046] 1. Experimental materials

[0047] Experimental subjects: Human pulmonary artery endothelial cells (HPAECs) were purchased from Science Cell Company. The sixth-generation HPAECs in logarithmic growth phase were used. They were inoculated after trypsin digestion. When the cells grew to about 70%, serum-free DMEM was used. Low-glucose medium was starved for 18 h, then normoxia, hypoxia, hypoxia + drug intervention respectively.

[0048] Main reagents: Cordyceps acid, fetal bovine serum, PBS, trypsin, DMEM low glucose medium, ECM complete medium, in situ apoptosis detection kit (Roche, Switzerland).

[0049] Main instruments: cell culture dish, cell incubator, 96-well plate, microscope, biological safety cabinet, alcohol lamp, Pasteur tube, 15ml / 50ml centrifuge tube, pipette, alcohol cotton ball, microplate reader.

[0050] 2. Experimental method

[0051] Inoculate HPAEC cells in logarithmic...

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Abstract

The invention relates to an application of ophiocordyceps sinensis to preparation of medicines for preventing or treating pulmonary hypertension, belongs to the technical fields of resources of Chinese medicinal materials, natural medicines, clinical pharmacology and compound pharmacology, and provides the application of cordycepic acid to preparation of the medicines for preventing or treating pulmonary hypertension. The ophiocordyceps sinensis can promote proliferation of pulmonary artery endothelial cells, alleviates apoptosis of the pulmonary artery endothelial cells under the hypoxia condition, has obvious symptom alleviating effects on right ventricular hypertrophy, pulmonary vascular remodeling, pulmonary arteriole tube wall thickening and duct cavity constriction, and can be used for preparing the medicines for preventing or treating the pulmonary hypertension.

Description

technical field [0001] The invention relates to the application of cordycepin in the preparation of medicines for preventing or treating pulmonary arterial hypertension, and belongs to the technical fields of traditional Chinese medicine resources, natural medicines, clinical pharmacology and compound pharmacology. Background technique [0002] Pulmonary arterial hypertension (PAH) is a group of idiopathic pulmonary hemodynamic disorders caused by certain diseases, mainly manifested as increased pulmonary vascular resistance, pulmonary vascular remodeling, in situ thrombosis, and ultimately leading to right ventricular hypertrophy , and progressively develop into right heart failure until death, which is a very serious chronic pulmonary circulatory disease and a key link in pulmonary heart disease. Pulmonary arterial hypertension has a high morbidity and mortality rate, and a very poor prognosis. It is similar to malignant tumors, and there is no specific treatment drug or s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/047A61P11/00A61P9/12
CPCA61K31/047A61P9/12A61P11/00
Inventor 雷桅陈晓盈何原税晓容朱莹黄石安陈灿
Owner 雷桅