Chip for controlling dissolved oxygen concentration in microfluid

Abstract

The invention discloses a chip for controlling the dissolved oxygen concentration in a microfluid. The chip comprises a first layer, a silica gel membrane barrier layer and a second layer which are sequentially overlapped, wherein the inner surface of the first layer and the inner surface of the second layer are separately adhered to two side surfaces of the middle silica gel membrane barrier layer in an overlapped mode, a first microchannel is formed by a groove which is formed in the inner surface of the first layer and the side surface, to which the first layer is adhered to, of the silicagel membrane barrier layer, and a second microchannel is formed by a groove which is also formed in the inner surface of the second layer and the other side surface, to which the second layer is adhered to, of the silica gel membrane barrier layer; the first microchannel is isolated from the second microchannel through the silica gel membrane barrier layer, the first microchannel is a unidirectional channel suitable for flowing of a cell culture medium, and the second microchannel is a unidirectional channel suitable for flowing of a Na2SO3 solution; and oxygen in the cell culture medium flowing in the first microchannel can pass through the isolation silica gel membrane and diffuse into the Na2SO3 solution flowing in the second microchannel. Quantitative control of the dissolved oxygen concentration in the microfluid can be achieved through the chip.

Description

technical field [0001] The invention relates to a microfluidic dissolved oxygen concentration control chip. Background technique [0002] Oxygen is an electron acceptor necessary for energy metabolism in higher animals, and it controls cell metabolism, hormone secretion and gene expression, etc. In tissues, oxygen levels are maintained through delicate, highly conserved intracellular structures and organ-level feedback loops, and oxygen homeostasis in vivo is extremely important for maintaining physiological functions of cells (Citation: Park, Bansal et al. 2006). In physiological liver lobules, HA and PV blood have different dissolved oxygen concentrations (cited: Jungermann and Kietzmann 2000), HA blood dissolved oxygen concentration is 3.3-4.7 mg / L (104-146 μmol / L), PV blood dissolved oxygen concentration is 1.5-2.0mg / L (48-64μmol / L). The dissolved oxygen concentration currently used in two-dimensional or three-dimensional in vitro cell culture is higher than that in b...

AI Technical Summary

Problems solved by technology

Park et al. embed microelectrode arrays in microchannels, use electrolysis of water to generate oxygen, use oxygen-sensitive fluorescent membranes to measure and visualize oxygen concentration gradients, and change the spatiotemporal distribution of oxygen gradients by controlling the current of microelectrodes. However, based on electrochemical The oxygen chip design of this method is relatively complicated, which is not conducive to experimental operation and quantitative control

Skolimowski et al. designed an oxygen gradient generation chip based on microfluidic technology. The chip includes a cell culture chamber, an oxygen gradient generator and an optical sensor. Oxygen diffuses through a gas permeable membrane (PDMS) and is consumed in sulfite. The integrated The fluorescent dye on the chip is used to detect the oxygen concentration, however, the flow rate of the medium during the experiment is as low as 10 μL / min, and when the medium flow rate exceeds 50 μL / min, no obvious oxygen concentration is produced in the chip gradient

Polinkovsky et al. designed two microfluidic chips, using the gas mixing channel network to achieve different oxygen concentration gradients. The first chip uses the mixture of pure nitrogen and pure oxygen to produce an oxygen concentration gradient that varies linearly from 0 to 100%. The two types of chips use the mixture of nitrogen and air to produce an exponentially changing oxygen concentration gradient of 0-20.9%. However, the oxygen chip of the gas mixing method tends to generate a large number of bubbles, which is not conducive to the stability of the fluid.

In addition, based on the current oxygen chip design, none of them can simulate the dissolved oxygen concentration of arterial and venous fluids at physiological levels.

Images