Detection method and detection reagent kit for nephrotic syndrome and hemorrhagic fever

Abstract

The invention provides a method for detecting hemorrhagic fever with renal syndrome by a gold drop method and a detection kit. In this method, the anti-Hantavirus IgG antibody in the serum is used as the detection object, and it includes the steps of: (a) coating the purified Hantavirus nucleoprotein on a nitrocellulose membrane to form a coating in the sampling area. A nitrocellulose detection membrane with hantavirus nucleoprotein; (b) using non-human anti-human IgG antibody and colloidal gold as raw materials to prepare a conjugate of colloidal gold-anti-human IgG antibody; (c) the Serum sample is added dropwise on the spotting area of ​​the detection membrane obtained in step (a); (d) washing the spotting area of ​​the detection membrane of step (c); (e) colloidal gold-antihuman The conjugate of the IgG antibody is dropped on the spotting area; (f) washing the spotting area of ​​the detection membrane in step (e). The method and kit can detect hemorrhagic fever with renal syndrome rapidly, correctly and conveniently.

Description

technical field

[0001] The present invention relates to the fields of epidemiology, molecular biology and immunology. More specifically, the present invention relates to a detection method and a detection kit for hemorrhagic fever with renal syndrome. Background technique

[0002] Hemorrhagic fever with renal syndrome (HFRS) is an acute infectious disease in humans caused by certain viruses in the genus Hantavirus of the Kenyaviridae family. The mortality rate is high after the onset, so early correct diagnosis is very necessary. In my country, hemorrhagic fever with renal syndrome (HFRS) is the most harmful infectious disease except viral hepatitis. The scope of the epidemic area of ​​this disease involves Europe, Asia, Africa and other parts of the world, and the number of cases in my country has no decreasing trend. In the Pudong area of ​​Shanghai, due to the increase of the foreign population, the incidence of HFRS is mostly high. Therefore, the early rapid diagnosi...