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Lactobacillus salivarius cjls1511, animal feed additive composition comprising same bacterium or dead cells thereof, and method for producing same dead cells

A CJLS1511, Lactobacillus salivarius technology, applied in animal feed, animal feed, microbial-based methods, etc., to achieve the effects of improving immune status, excellent neutral lipid degradation, and enhancing animal growth performance

Active Publication Date: 2019-04-02
CJ CHEILJEDANG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, the problem of antibiotic resistance has been discussed worldwide due to its overuse

Method used

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  • Lactobacillus salivarius cjls1511, animal feed additive composition comprising same bacterium or dead cells thereof, and method for producing same dead cells
  • Lactobacillus salivarius cjls1511, animal feed additive composition comprising same bacterium or dead cells thereof, and method for producing same dead cells
  • Lactobacillus salivarius cjls1511, animal feed additive composition comprising same bacterium or dead cells thereof, and method for producing same dead cells

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Experimental program
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Embodiment approach

[0047] According to another exemplary embodiment of the present invention, there is provided a method for preparing inactivated bacterial cells of Lactobacillus salivarius CJLS1511 (KCCM11829P), including:

[0048] Cultivate Lactobacillus salivarius CJLS1511 (KCCM11829P) to prepare a culture solution,

[0049] Heat the culture medium at a temperature of 70 to 160°C,

[0050] Cool the heated broth to a temperature of 10 to 60°C, and

[0051] The inactivated bacterial cells of Lactobacillus salivarius CJLS1511 (KCCM11829P) were separated from the cooled culture broth.

[0052] Specifically, in the step of culturing Lactobacillus salivarius CJLS1511 (KCCM11829P) to prepare a culture solution, an agar medium, specifically, MRS medium may be used. The culturing may be performed at 25°C to 40°C for 5 to 48 hours, more specifically at 30 to 40°C for 12 to 36 hours, and more specifically at 35 to 40°C for 20 to 30 hours, thereby preparing a culture solution.

[0053] Then, the culture solution ...

Embodiment 1-6

[0066] Using Lactobacillus salivarius KCCM 40210 known in the art as a reference strain, the safety, acid resistance, bile resistance, antimicrobial activity, digestive enzyme activity and triglycerides of the Lactobacillus salivarius CJLS1511 (KCCM11829P) strain of the present invention were evaluated as follows Degradation activity.

experiment example 1

[0067] Experimental example 1: Safety evaluation of Lactobacillus salivarius CJLS1511 (KCCM11829P)

[0068] In order to evaluate the safety of Lactobacillus salivarius CJLS1511 (KCCM11829P) strain, hemolysis test, gelatin liquefaction test, confirmation of the occurrence of harmful metabolites (ammonia) and phenylalanine were carried out according to the safety evaluation test method proposed by the Korean Biotechnology Industry Organization Standard Deamination test. The results are shown in Table 3 below.

[0069] [table 3]

[0070]

[0071] As confirmed in Table 3, it can be found that Lactobacillus salivarius CJLS1511 (KCCM11829P) was negative in the confirmation of gelatin liquefaction test, phenylalanine deamination test and ammonia test, and it was safe in the hemolysis test.

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Abstract

The present invention relates to an animal feed additive composition comprising a novel Lactobacillus salivarius CJLS1511 strain or dead cells thereof, and to a method for producing the dead cells.

Description

Technical field [0001] The present invention relates to a new type of Lactobacillus salivarius CJLS1511, a composition for adding animal feed containing the bacterium or its inactivated bacterial cells, and a method for preparing inactivated bacterial cells. Background technique [0002] Microorganisms of the genus Lactobacillus are lactobacilli that undergo homo-fermentation or hetero-fermentation, and are usually present in the intestines of animals including humans, and in the fermentation process of dairy products and vegetables. Microorganisms of the genus Lactobacillus are lactic acid-producing bacteria commonly found in the intestines of animals, and use dairy products and vegetables as their substrates for homo-fermentation or hetero-fermentation. It is known that Lactobacillus microorganisms maintain an acidic environment in the intestinal tract in animals, inhibit the overgrowth of harmful bacteria such as Escherichia coli and Clostridium, improve animal diarrhea and co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/16A23K50/75C12R1/225
CPCC12N1/20A23K10/16A23K50/75C12R2001/225C12N1/205A23K10/18C12N2523/00A23V2400/181A61K35/747
Inventor 金智恩蔡炅洙金星勋池硕祐李重受
Owner CJ CHEILJEDANG CORP
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