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A kind of Escherichia coli transformant that absorbs heavy metals in water and its application

A technology of Escherichia coli and transformants, applied in the field of bioengineering, can solve problems such as time-consuming and low efficiency

Active Publication Date: 2020-08-25
WENZHOU MEDICAL UNIV
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  • Application Information

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Problems solved by technology

However, most heavy metal adsorption strains express metal-specific adsorption proteins, so they can only adsorb certain heavy metals
However, environmental water, especially industrial sewage, often contains a large amount of multiple heavy metals at the same time. If a single heavy metal is used to adsorb bacterial strains, each heavy metal needs to be removed in turn, which takes a long time and is inefficient.

Method used

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  • A kind of Escherichia coli transformant that absorbs heavy metals in water and its application
  • A kind of Escherichia coli transformant that absorbs heavy metals in water and its application
  • A kind of Escherichia coli transformant that absorbs heavy metals in water and its application

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Embodiment Construction

[0034] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings.

[0035] 1. Materials

[0036] 1.1. Strains

[0037] E.coli BL21(DE3)[F-ompT hsdS(rB-mB-)gal dcm(DE3)], hereinafter referred to as BL21 strain, is preserved by our laboratory.

[0038] 1.2. Plasmids

[0039] pET28b[Kanar], containing His tag and T7 terminator, is preserved by our laboratory.

[0040] 1.3. M9 medium

[0041] On the basis of M9 basal medium, 20 kinds of natural amino acid mixed aqueous solution (10 mg / L / type) and vitamin B1 aqueous solution (5 μg / mL) were added, and stored at 4°C for later use.

[0042] 1.4. Main Reagents

[0043] PCR amplification polymerase 2×phanta was purchased from Vazyme; the seamless cloning reaction kit was purchased from Heyuan Biotechnology (Shanghai) Co., Ltd.

[0044] Cobalt...

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Abstract

The invention discloses an escherichia coli transformant adsorbing heavy metals in water body and application thereof. The transformant is obtained by transforming escherichia coli by plasmid pET-Lpp-OmpA-IscA, and the plasmid contains an expression vector pET-28b, an escherichia coli IscA protein gene, an escherichia coli prolipoprotein signal peptide Lpp gene and an escherichia coli membrane protein OmpA gene. According to a genetic engineering technology, the plasmid is obtained, and is constructed into the escherichia coli to express fusion protein Lpp-OmpA-IscA on the outer surface of anescherichia coli cytomembrane so as to construct a strain capable of efficiently adsorbing various heavy metals in water.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an Escherichia coli transformant for absorbing heavy metals in water and an application thereof. Background technique [0002] With the acceleration of industrialization, the discharge of heavy metal wastewater is huge, and heavy metal pollution is becoming more and more serious. Heavy metal pollution in water has become one of the major environmental problems today, so effective treatment of it is an urgent task to be solved. [0003] Biosorption is to use the chemical composition and structural characteristics of bacteria, fungi, algae and other biological materials to absorb and accumulate heavy metals in wastewater, so as to achieve the purpose of removing heavy metals in wastewater. In recent years, more and more whole-cell microbial strains that can effectively adsorb heavy metals have been developed, such as adsorption engineering strains for mercury, l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12N15/70C12N15/66C02F3/34C12R1/19C02F101/20
CPCC02F3/34C02F2101/20C07K14/245C12N15/66C12N15/70
Inventor 王伍章丽和姜风英程荣
Owner WENZHOU MEDICAL UNIV
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