A kind of Escherichia coli transformant that absorbs heavy metals in water and its application
A technology of Escherichia coli and transformants, applied in the field of bioengineering, can solve problems such as time-consuming and low efficiency
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[0034] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings.
[0035] 1. Materials
[0036] 1.1. Strains
[0037] E.coli BL21(DE3)[F-ompT hsdS(rB-mB-)gal dcm(DE3)], hereinafter referred to as BL21 strain, is preserved by our laboratory.
[0038] 1.2. Plasmids
[0039] pET28b[Kanar], containing His tag and T7 terminator, is preserved by our laboratory.
[0040] 1.3. M9 medium
[0041] On the basis of M9 basal medium, 20 kinds of natural amino acid mixed aqueous solution (10 mg / L / type) and vitamin B1 aqueous solution (5 μg / mL) were added, and stored at 4°C for later use.
[0042] 1.4. Main Reagents
[0043] PCR amplification polymerase 2×phanta was purchased from Vazyme; the seamless cloning reaction kit was purchased from Heyuan Biotechnology (Shanghai) Co., Ltd.
[0044] Cobalt...
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