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Method for producing bacitracin through fermentation

A technology of bacitracin and fermentation broth, applied in the field of peptide drug manufacturing, can solve the problems of bacitracin destruction, lowering the final titer of bacitracin, etc., and achieve the effects of simple operation, short preparation period and low isolation titer

Active Publication Date: 2019-04-12
JIANGSU JIUYANG BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this preparation method reduces the production cost, the bacitracin produced by it is easily oxidized and destroyed, thereby greatly reducing the final potency of bacitracin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for producing bacitracin by fermentation, comprising the following steps:

[0027] 1) Fermentation broth pretreatment: take a fermentation broth with a weight of 1 ton, add 50 kg of sodium thiosulfate to the fermentation broth and stir evenly, then pump the fermentation broth into a pretreatment tank, add oxalic acid to adjust the pH to 2.5-3.0, stirring for 1h;

[0028] 2) Resin adsorption: add ammonia water to the fermented broth acidified in step 1 to adjust the pH to 4.0-4.5, and detect the titer of bacitracin in the fermented broth, and put in 1600L of 001×1.1-H + The adsorption resin adsorbs bacitracin for 5 hours, so that the content of bacitracin in the adsorption tail liquid does not exceed 50U / ml;

[0029] 3) Resin separation and rinsing: The resin after adsorption is separated from saturated resin and feed liquid by a resin separator, and then rinsed with deionized water until the water is clear and free of floating objects;

[0030] 4) Analytical ...

Embodiment 2

[0034] A method for producing bacitracin by fermentation, comprising the following steps:

[0035] 1) Fermentation broth pretreatment: take a fermentation broth with a weight of 1 ton, add 35 kg of sodium thiosulfate to the fermentation broth and stir evenly, then pump the fermentation broth into a pretreatment tank, add oxalic acid to adjust the pH to 2.5-3.0, stirring for 1h;

[0036] 2) Resin adsorption: add ammonia water to the acidified fermentation broth in step 1 to adjust the pH to 4.0-4.5, and detect the potency of bacitracin in the fermentation broth, and put 1400L of 001×1.1-H + The adsorption resin adsorbs bacitracin for 5 hours, so that the content of bacitracin in the adsorption tail liquid does not exceed 50U / ml;

[0037] 3) Resin separation and rinsing: The resin after adsorption is separated from saturated resin and feed liquid by a resin separator, and then rinsed with deionized water until the water is clear and free of floating objects;

[0038] 4) Analyt...

Embodiment 3

[0042] A method for producing bacitracin by fermentation, comprising the following steps:

[0043] 1) Fermentation broth pretreatment: take a fermentation broth with a weight of 1 ton, and add 55 kg of sodium thiosulfate to the fermentation broth and stir evenly, then pump the fermentation broth into a pretreatment tank, add oxalic acid to adjust the pH to 2.5-3.0, stirring for 1h;

[0044] 2) Resin adsorption: add ammonia water to the fermented broth acidified in step 1 to adjust the pH to 4.0-4.5, and detect the potency of bacitracin in the fermented broth, and put 1600L of 001×1.1-H + The adsorption resin adsorbs bacitracin for 4.5 hours, so that the content of bacitracin in the adsorption tail liquid does not exceed 50U / ml;

[0045]3) Resin separation and rinsing: The resin after adsorption is separated from saturated resin and feed liquid by a resin separator, and then rinsed with deionized water until the water is clear and free of floating objects;

[0046] 4) Analyti...

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PUM

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Abstract

The invention discloses a method for producing bacitracin through fermentation. The method comprises six steps of pretreating fermentation liquor, performing resin adsorption, performing resin separation and rinsing, performing resolution and ammonia removal, performing nanofiltratoin for the first time and performing extraction and concentration. According to the prepared bacitracin, sodium thiosulfate is added, and airtight operation is performed, so that the bacitracin cannot be oxidized, the final potency of the bacitracin can be tremendously improved, and the medical value of the bacitracin is higher; and besides, the operation is simple, the preparation cycle is short, and the method can be used for massive industrial production of the bacitracin.

Description

technical field [0001] The invention relates to the technical field of peptide drug production, in particular to a method for fermenting and producing bacitracin. Background technique [0002] Bacitracin, with the chemical formula C66H103N17O16S, is a white or light yellow powder. Its antibacterial spectrum is similar to that of penicillin. Both have bactericidal effect, so they have high medical value. However, there are still various deficiencies in the modern large-scale production process, such as high production cost, low final titer of bacitracin, and long production cycle. [0003] The invention with the patent number CN106893751A discloses a method for producing bacitracin by fermentation. By optimizing the organic compound nitrogen source and fermentation conditions, the method ensures the stability of the fermentation unit between batches, reduces the content of impurity F and the synthesis of bacitracin During this period, superparamagnetic microspheres are adde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/58C12P21/04
CPCC12P21/02C07K7/58
Inventor 李军郭绍定唐静黄晔柴小涛明国军
Owner JIANGSU JIUYANG BIOLOGICAL PHARMA
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