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A detection method for spice additives in formula foods for special medical purposes

A technology for formula food and detection method, applied in measurement devices, scientific instruments, material separation and other directions, can solve the problems of weak mutagenic activity, cell death, dyspnea, etc., achieves less interference, simplified pretreatment steps, and high recovery rate Effect

Active Publication Date: 2021-10-08
ZHOUSHAN INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of vanillin and other additives in food for special medical needs is essential, and the safety of vanillin and other additives is still controversial at home and abroad. The use of large doses of vanillin and other additives has greater harm to the human body. Excessive intake can lead to headaches, nausea, vomiting, dyspnea, and even liver and kidney damage. At the same time, maltol has weak mutagenic activity and toxic effects of inducing cell death, etc.

Method used

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  • A detection method for spice additives in formula foods for special medical purposes
  • A detection method for spice additives in formula foods for special medical purposes
  • A detection method for spice additives in formula foods for special medical purposes

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Embodiment Construction

[0027] In order to illustrate the content of the present invention more clearly, specific examples are used to illustrate the following, and the specific examples do not limit the scope of the content of the present invention.

[0028] Instruments and reagents

[0029] Agilent1260LC liquid chromatograph (Agilent Corporation of the United States), configuration diode array detector (DAD); Chromatographic column: Poroshell 120SB-Aq, (4.6 × 100mm, 2.7μm); UV-2700 ultraviolet-visible spectrophotometer (Shimadzu Enterprise Management (China) Co., Ltd.) BS-124S ​​1 / 100,000 electronic balance (Beijing Sartorius Instrument System Co., Ltd.); FC204 1 / 100,000 electronic balance (Shanghai Precision Scientific Instrument Co., Ltd.); H20-I-2-TOC -T ultrapure water device (Sartorius Scientific Instrument Co., Ltd.); AllegraX-30R centrifuge (Beckman Coulter Co., Ltd.); MS3 vortex mixer (Germany IKA company); SPE column: (500mg / 6ml), (500mg / 6ml), Welchrom P-WAX, Captive EMR-Lipid small co...

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Abstract

A method for the detection of spice additives in formula foods for special medical purposes. Through a solid-phase extraction technique, the appropriate extraction method Captive EMR‑Lipid purification column is selected to purify the sample. No activation and equilibrium are required before loading the sample, and the sample solution is directly Loaded to the SPE column to adsorb carbon chains of C5 and above in the lipids of FSMA samples, thereby removing lipids and protein interferers, and achieving the purpose of purifying 11 target fragrance additives. The small column does not contain a solvent retention filter element, and can realize gravity flow, which simplifies the sample pretreatment steps, shortens the pretreatment time, and reduces the interference of sample impurity peaks, thereby significantly improving the reliability and practicability of the method. Through Captive EMR‑ Lipid (enhanced lipid removal filter column) is purified in the form of solid phase extraction SPE and detected by high performance liquid chromatography. It has the advantages of high recovery rate, simplicity and speed, and can meet the daily limit of spice compounds in food for special medical treatment. control requirements.

Description

technical field [0001] The invention relates to the technical field of food additive detection, in particular to a method for detecting flavor additives in formula food for special medical purposes. Background technique [0002] Formula food for special medical purposes (FSMA, hereinafter referred to as food for special medical purpose) is a formula food specially processed and formulated to meet the special needs of nutrients or diet for people with restricted eating, digestive and absorption disorders, metabolic disorders or specific disease states. The main ingredients are biologically active ingredients such as protein, fat, carbohydrates, and vitamins. It is an emerging food industry in China. However, the use of vanillin and other additives in food for special medical treatment is essential. The safety of vanillin and other additives is still controversial at home and abroad. The use of large doses of vanillin and other additives has greater harm to the human body. Ex...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/062G01N2030/143
Inventor 吴纤愫
Owner ZHOUSHAN INST FOR FOOD & DRUG CONTROL
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