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Production process of L-ornithine hydrochloride

A kind of technology of ornithine hydrochloride and production process, which is applied in the field of amino acid preparation, can solve the problems of long time, difficult to remove and clean, unfavorable to industrial production, etc., achieve low cost, save production cost, and shorten the length of industrial production Effect

Inactive Publication Date: 2019-04-19
NANTONG ZILANG BIOPHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neutralize with dilute sulfuric acid to remove Ba 2+ , concentrated and crystallized to obtain L-ornithine hydrochloride crystalline crude product, the crude product was recrystallized in pure water to obtain pure product, and the mother liquor was separated and purified by ion-exchange resin chromatography to obtain L-ornithine hydrochloride again. The method is Synthesis of L-ornithine hydrochloride by chemical method, introducing foreign ion Ba 2+ , it is difficult to remove cleanly in the process of industrial production, followed by ion exchange resin for chromatographic separation and purification, which takes a long time and is not conducive to industrial production

Method used

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  • Production process of L-ornithine hydrochloride

Examples

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Embodiment 1

[0025] A production process of L-ornithine hydrochloride, comprising the following steps:

[0026] Step 1, preparation of L-arginine solution: put 2500kg of ultrapure water and 1125kg of L-arginine into the reaction kettle, turn on the stirrer at a speed of 1500r / min, mix well to prepare L-arginine solution, and then add 1mol / L hydrochloric acid to adjust the pH of the L-arginine solution to 8.0~9.0.

[0027] Step 2: Preparation of L-ornithine hydrochloride by enzymatic hydrolysis: Turn on the circulating water, lower the temperature of the L-arginine solution prepared in step 1 to 25°C, and then add 2g / L L-arginine to the reaction kettle Nitase and 225kg of phase transfer catalyst were used for enzymatic hydrolysis to prepare L-ornithine hydrochloride. After 6 hours of reaction, the liquid phase was sampled for detection.

[0028] Step 3, concentration of L-ornithine hydrochloride: filter the qualified solution in step 2 to obtain filtrate and filter residue, store the filte...

Embodiment 2

[0031] A production process of L-ornithine hydrochloride, comprising the following steps:

[0032] Step 1, preparation of L-arginine solution: put 2500kg of ultrapure water and 1000kg of L-arginine into the reactor, turn on the stirrer at a speed of 1500r / min, mix well to prepare L-arginine solution, and then add 0.5mol / L hydrochloric acid to adjust the pH of the L-arginine solution to 8.0~9.0.

[0033] Step 2: Preparation of L-ornithine hydrochloride by enzymatic hydrolysis: Turn on the circulating water, lower the temperature of the L-arginine solution prepared in step 1 to 20°C, and then add 2.5g / L L- Arginase and 100kg of phase transfer catalyst were used for enzymatic hydrolysis to prepare L-ornithine hydrochloride. After 5 hours of reaction, the liquid phase was sampled for detection.

[0034] Step 3, concentration of L-ornithine hydrochloride: filter the qualified solution in step 2 to obtain filtrate and filter residue, store the filter residue in a refrigerator at 4°...

Embodiment 3

[0037] A production process of L-ornithine hydrochloride, comprising the following steps:

[0038] Step 1, preparation of L-arginine solution: put 2500kg of ultrapure water and 1250kg of L-arginine into the reaction kettle, turn on the stirrer at a speed of 3000r / min, mix well to prepare L-arginine solution, and then add 0.75mol / L hydrochloric acid to adjust the pH of the L-arginine solution to 8.0~9.0.

[0039] Step 2: Preparation of L-ornithine hydrochloride by enzymatic hydrolysis: Turn on the circulating water, lower the temperature of the L-arginine solution prepared in step 1 to 22°C, and then add 4g / L L-arginine to the reaction kettle Nitase and 300kg of phase transfer catalyst were used for enzymatic hydrolysis to prepare L-ornithine hydrochloride. After 7 hours of reaction, the liquid phase was sampled for detection.

[0040]Step 3, concentration of L-ornithine hydrochloride: filter the qualified solution in step 2 to obtain filtrate and filter residue, store the fil...

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Abstract

The invention provides a production process of L-ornithine hydrochloride. The production process mainly includes the following steps: preparation of an L-arginine solution, preparation of L-ornithinehydrochloride by enzymatic hydrolysis, condensation of the L-ornithine hydrochloride and refining of the L-ornithine hydrochloride. The production process of the invention is simple in operation for the preparation of the L-ornithine hydrochloride by enzymatic hydrolysis, and L-arginase can be recycled after reaction. The production process of the L-ornithine hydrochloride refines the L-ornithinehydrochloride with ethanol, and the prepared L-ornithine hydrochloride has a purity of more than 80%. The production process is simple in operation, high in purity and suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to a preparation method of amino acid, in particular to a production process of L-ornithine hydrochloride. Background technique [0002] Developing amino acid production to meet the growing needs of my country's medical care industry is a long-term goal pursued by some governments and competent departments in our country. The production process of amino acids includes chemical synthesis, microbial fermentation powder, natural protein extraction, enzymatic synthesis and other ways. In recent years, L-ornithine, as a free multifunctional amino acid, is an important basic amino acid with both nutritional and therapeutic functions. The application of L-ornithine in the medical field is mainly manifested in the role of liver protection; it can promote the recovery of patients; it can resist shock caused by endotoxin, limit arginine and supplement L-ornithine can improve guanidine Deficiency of acetate methyltransferase, etc. The app...

Claims

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Application Information

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IPC IPC(8): C07C227/40C07C227/42C07C229/26C12P13/10
CPCC07B2200/07C07C227/40C07C227/42C12P13/10C07C229/26
Inventor 曹晓霞秦振华
Owner NANTONG ZILANG BIOPHARMA TECH CO LTD
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