Cyclic dipeptide compound and preparation method and application thereof
A cyclic dipeptide and compound technology, applied in the field of cyclic dipeptide compounds and their preparation, can solve the problem that there are not many cyclic peptide compounds, and achieve the effect of wide application prospect and widening research field.
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Embodiment 1
[0046] 20L of strain F8 was fermented with YMG agar medium, and cultured upside down at 28-30°C for 14 days. Cut the agar medium into small pieces and soak them overnight in the conical flask with the extract solution (ethyl acetate:methanol:glacial acetic acid =80:15:5 V / V), repeat three times. The soaking solution was concentrated under reduced pressure (42°C) to dryness to obtain the crude extract of secondary metabolites of the strain. The crude extract was first dissolved repeatedly with ethyl acetate 3 times to collect the solution, and the insoluble part was dissolved in water, then extracted three times with an equal volume of ethyl acetate to collect the ethyl acetate phase, and combined with the previous ethyl acetate solution to obtain Ethyl acetate phase. The ethyl acetate phase was evaporated to dryness under reduced pressure at 42-60° C. to obtain ethyl acetate extract. The ethyl acetate extract was then extracted 3-5 times with equal volumes of methanol and pe...
Embodiment 2
[0053] Example 2 (inhibitory effect of 4 compounds on lung cancer cell invasion)
[0054] [Material] 1. Instrument: CO 2 Incubator, inverted microscope, clean bench, alcohol lamp; 2. Reagents and consumables: DMEM medium, fetal bovine serum, Transwell chamber (24-well, 8.0-um pore size), 24-well plate, PBS solution, 0.25% Trypsin solution, ice methanol, 0.1% crystal violet staining solution, small tweezers, cotton swabs, glass slides, coverslips, etc. 3. Lung cancer cells (A549)
[0055] [step]
[0056] 1. Starvation treatment: culture the cells to the logarithmic growth phase, and starve the cells in serum-free medium for 12-24 hours;
[0057] 2. Collect cells: Take out the cells after starvation treatment, discard the medium, wash twice with PBS, trypsinize, add medium to resuspend the cells and transfer to a centrifuge tube; centrifuge at 800rpm for 2min, discard the supernatant; wash with PBS After one time, add 3 mL of serum-free medium to resuspend the cells;
[005...
Embodiment 3
[0069] [Material] 1. Instrument: CO 2 Incubator, inverted microscope, clean bench, alcohol lamp 2, reagents and consumables: DMEM medium, fetal bovine serum, BioCoat basement membrane matrix membrane-coated invasion chamber (24-well, 8.0-μm pore size), 24-well plate , PBS solution, 0.25% trypsin solution, ice methanol, 0.1% crystal violet staining solution, small tweezers, cotton swabs, glass slides, coverslips, etc.
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