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Cervical cancer molecular marker and application thereof

A cervical cancer and sequence technology, which is applied in the field of tumor gene detection to achieve the effect of good application prospect, good stability and accurate detection method

Active Publication Date: 2019-04-23
上海市长宁区妇幼保健院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research showing whether hsa_circ_0020594 is related to cervical cancer. The inventors further analyzed the hsa-let-7c-5p interacting with hsa_circ_0020594 and the target gene of hsa-let-7c-5p, and constructed the hsa_circ_0020594 biological information regulation network

Method used

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  • Cervical cancer molecular marker and application thereof
  • Cervical cancer molecular marker and application thereof
  • Cervical cancer molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Data screening and integrated analysis

[0037] GEO (Gene Expression Omnibus) database is developed and maintained by NCBI (National Center for Biotechnology Information). GEO database is the largest database of gene expression data. Develop dataset inclusion criteria:

[0038] ①The selected dataset must be the whole genome mRNA / miRNA / circRNA transcriptome data;

[0039] ②These data are from the tissues of cervical squamous cell carcinoma (CSCC) case group and control group (N) (without drug stimulation or transfection);

[0040] ③All standardized or original datasets were considered in this study.

[0041] After filtering, the following datasets were obtained:

[0042] Table 1 List of datasets that were screened for inclusion

[0043]

[0044] data analysis:

[0045] The tool used was R-3.3.3, the metaMA package was used for analysis, and the method used to combine the p-values ​​in the meta-analysis was the inversenormal method.

[0046] Differentia...

Embodiment 2

[0051] Example 2 RT-PCR verification of the relative expression of hsa_circ_0020594 in cervical squamous cell carcinoma tissue and adjacent tissue

[0052] 1. Materials

[0053] Tissue samples from 27 patients with cervical squamous cell carcinoma who underwent surgery in the gynecological ward of the hospital were collected, and the corresponding paracancerous tissues of the patients were used as the control group. The pathological diagnosis of the patient was verified by a pathologist. None of the patients had received radiotherapy or chemotherapy before surgery, and had no other surgical, endocrine, metabolic, infectious and other gynecological diseases such as uterus and ovary. Fresh tissue samples were obtained under sterile conditions, washed with saline to remove blood, and then placed into RNase-free cryopreservation tubes, quick-frozen in liquid nitrogen overnight, and frozen at -80°C for long-term storage.

[0054] 2. Experimental method

[0055] 2.1 Tissue total ...

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Abstract

The invention relates to a cervical cancer molecular marker and an application thereof, and particularly relates to an application of hsa_circ_0020594 and a related gene hsa-let-7c-5p thereof in diagnosing cervical cancer. The inventor further excavates, integrates and analyzes the sequencing data of the cervical cancer, finds out the key molecule hsa_circ_0020594 participating in the regulation and control of the cervical cancer and the related gene has-let-7c-5p, draws a biological information regulation network, thereby providing a new molecular diagnostic marker for the diagnosis of the cervical squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the technical field of tumor gene detection, in particular to cervical cancer-related circRNA molecular markers and their application in the preparation of diagnostic reagents, and more particularly to hsa_circ_0020594 and its related gene hsa-let-7c-5p in the diagnosis of cervical cancer Applications. Background technique [0002] Cervical cancer is one of the three major malignant tumors of the female reproductive system, which seriously threatens women's health. Early clinical symptoms are not obvious, and the main method of diagnosis, pathological biopsy, is invasive. At present, the recognized and reliable molecular markers of cervical cancer include three categories: DNA, RNA and protein. In recent years, scholars have found that more and more RNA molecular markers are abnormally expressed in cervical cancer, which are closely related to the clinicopathological characteristics, metastasis and invasion of cervical cancer...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
Inventor 马成斌刘平杨龙涛张文缨吴琼蔚刘彧
Owner 上海市长宁区妇幼保健院
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