Protective HPV (Human Papillomavirus) immunoglobulin of yolk (IgY) and application thereof in preventing and treating HPV

A yolk antibody, HPV16L1 technology, applied in the application of prevention/treatment of HPV infection, in the field of yolk antibody of recombinant microcircular DNA of HPVL1, can solve the problem of no effective treatment method for HPV virus, etc., achieve low production cost, strong specificity, antibody high potency effect

Inactive Publication Date: 2019-04-26
江苏润洁生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no precedent for HPV L1 yolk antibody obtained by immunization with nucleic acid antigen to prevent cervical cancer
[0008] At present, there is no effective treatment for intracellular HPV in cervical mucosa

Method used

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  • Protective HPV (Human Papillomavirus) immunoglobulin of yolk (IgY) and application thereof in preventing and treating HPV
  • Protective HPV (Human Papillomavirus) immunoglobulin of yolk (IgY) and application thereof in preventing and treating HPV

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The preparation of embodiment 1 yolk antibody

[0068] 1. Preparation of recombinant microcircle DNA

[0069] 1) Design and synthesize HPV-L1 recombinant fragments with BglII and SalI restriction sites at both ends to obtain the target gene;

[0070] 2) Digest the above target gene fragment and the ZY781 vector with BglII and SalI at the same time, and clone the target fragment into the multi-cloning site of the vector ZY781 according to the conventional method after gel recovery to obtain a recombinant plasmid, which is the parental plasmid (PP) ;

[0071] 3) Transform Escherichia coli ZYCY10P3S2T with the above-mentioned parental plasmid, culture overnight, extract the plasmid, and then verify the insertion of the correct fragment by electrophoresis and sequencing of the plasmid;

[0072] 4) Inoculate the Escherichia coli with correct sequencing above into TB medium containing 50 μg / ml kana, culture overnight at 37°C and 250 rpm;

[0073] 5) Take the overnight cult...

Embodiment 2

[0084] Example 2 Gel for preventing / treating HPV infection and its effect evaluation

[0085] 1. Ingredients: HPV L1 chicken yolk antibody liposome microcapsules (nano-scale), glycerin, water, lactose, polyhexamethylene hydrochloride, adjust the pH to 5.0 after mixing.

[0086] Wherein, the liposome microcapsule (nanoscale) of HPV L1 chicken egg yolk antibody is obtained by freeze-drying and ultrafine pulverizing the egg yolk antibody obtained in Example 1 to obtain nanosized egg yolk antibody. The liposome microcapsule (nanoscale) is prepared by further encapsulating with lecithin.

[0087] Use the vaginal gel formulation to infuse highly active anti-HPV antibodies into patients, and realize the transmembrane transport of the antibodies through the nanoliposome method, so that the antibodies can directly neutralize the HPV virus in the cells, which solves the current clinical problem of HPV in the cervical mucosa cells The conundrum of no effective treatment for the virus. ...

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Abstract

The invention belongs to the field of bio-medicines, and specifically relates to an HPV (Human Papillomavirus) L1 recombinant annulet DNA immunoglobulin of yolk (IgY) and application thereof in preparing a drug for preventing / treating HPV infection. The HPV L1 recombinant annulet DNA comprises an L1 gene segment of HPV16 and a signal peptide sequence, and is taken as a nucleic acid antigen for directly immunizing a laying hen to obtain the IgY. Bio-activity of the IgY obtained by intramuscular injection of the DNA nucleic acid antigen for immunizing the laying hen is much higher than that of the IgY prepared by a common technology (subcutaneous injection of the protein antigen), valence of an antibody is higher, and specificity is stronger. The IgY can be used for preparing the drug for preventing / treating the HPV infection; a vagina drug inputs the high-activity anti-HPV antibody to a HPV positive patient, and a nano-liposome method is combined, so that the antibody gets across the film to enter into cells to directly neutralize HPV viruses in the cells. The IgY is of great significance for preventing cervical cancer.

Description

[0001] This patent is a divisional application for the invention of "protective HPV yolk antibody and its application". The original application number is: CN201810186327.0, and the application date is 20180307. technical field [0002] The invention belongs to the field of biomedicine, and in particular relates to an egg yolk antibody of HPV L1 recombinant microcircular DNA and its application in preventing / treating HPV infection. Background technique [0003] Cervical cancer accounts for the second place among female gynecological malignancies, and has now become an important disease that threatens women's health. In China, nearly 50,000 people die from cervical cancer every year. The etiology of cervical cancer has always been valued by scholars at home and abroad. The key to cervical cancer is HPV infection. HPV is a spherical, double-stranded circular DNA virus belonging to the genus Papillomavirus of the family Papillomaviridae. HPV infects humans mainly through direc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/62C07K16/02C07K16/08A61K39/42A61K9/127A61K31/785A61P31/20A61P31/02A61P35/00
CPCA61K9/0034A61K9/127A61K31/785A61K39/42A61K2039/505A61P31/02A61P31/20A61P35/00C07K14/005C07K16/02C07K16/084C07K2317/11C07K2319/02C07K2319/21C12N15/85C12N2710/20022C12N2800/106A61K2300/00
Inventor 庞以芹谢亦武吴必杰魏勇
Owner 江苏润洁生物科技有限公司
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