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DNA extraction method

An extraction method and technology of an extraction device, which are applied in the fields of DNA extraction and biotechnology, can solve the problems of low extraction purity, high price of an automatic nucleic acid extraction instrument, and complicated operation steps for DNA extraction by magnetic bead method, thereby saving experimental time, The effect of simplifying the DNA extraction process and reducing skill requirements

Active Publication Date: 2019-04-30
苏州含光微纳科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The operation steps of DNA extraction by magnetic bead method are cumbersome and the extraction purity is lower than that of silica gel membrane adsorption method. The price of fully automatic nucleic acid extraction instrument is beyond the tolerance of hospitals in remote areas and township health centers.

Method used

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Embodiment Construction

[0036] In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below through specific embodiments and accompanying drawings.

[0037] One. The DNA extraction device that the present invention adopts

[0038] The DNA extraction device used in the present invention includes a centrifuge and a DNA extraction centrifuge disc, and the DNA extraction centrifuge disc is installed on the centrifuge. Such as figure 1 As shown, the centrifuge disc 100 for DNA extraction in this embodiment is provided with several DNA extraction units 110 evenly distributed along the circumference. Wherein the solid line part indicates that it is located on the front of the centrifugal disc, and the dotted line part indicates that it is located on the back of the centrifugal disc. figure 2 The structure of the front of the centrifugal disc is shown separately, image 3 The structure on the back...

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Abstract

The invention relates to a DNA extraction method. The method includes the steps: blood and erythrocyte lysate are added into a first liquid inlet, a high concentration salt ion solution is added intoa second liquid inlet, and a DNA eluent is added into a third liquid inlet; the blood and the erythrocyte lysate in the first liquid inlet are mixed evenly; a centrifuge rotates at a rotating speed 1,and the solutions in the first liquid inlet and the second liquid inlet are allowed to enter a filter area and mixed; the centrifuge rotates at a rotating speed 2, DNA in the filter area is adhered to a DNA adsorbing material, remaining impurities enter a first liquid storage area, and the eluate in the third liquid inlet is allowed to enter a temporary liquid storage area; a centrifugal disk rotates at a rotating speed 3, the DNA eluate in the temporary liquid material area is allowed to enter the filter area, and impurities in the first liquid storage area enter the second liquid storage area; the centrifuge rotates at a rotating speed 4, and eluted DNA enters the first liquid storage area; and the DNA is taken out from the first liquid storage area. The DNA can be extracted efficiently.

Description

technical field [0001] The invention belongs to the technical fields of biotechnology and DNA extraction, and in particular relates to a DNA extraction method, which can realize DNA extraction conveniently and efficiently. Background technique [0002] The existing DNA extraction methods mainly include the following: [0003] 1) The classic phenol-chloroform method. Generally, it refers to the addition of an equal volume of phenol-chloroform mixture after cell lysis. According to the characteristics that DNA is easily soluble in water and not easily soluble in organic solvents, in an organic solvent environment, after protein denaturation precipitation and centrifugation, the organic solvent exists at the bottom of the test tube. , DNA exists in the upper layer of water, and the protein precipitate exists between the two liquids. Finally, the DNA is separated from the water with ethanol, precipitated, and recovered after centrifugation. The phenol-chloroform extraction pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1006C12N15/1017
Inventor 吴烨娴安光明陈兢
Owner 苏州含光微纳科技有限公司
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