Biocontrol seed coating agent for tomatoes and application of biocontrol seed coating agent

A technology of biocontrol and tomato, which is applied in the direction of application, biocide, biocide, etc., can solve the problems of large chemical control pollution and low efficiency of agricultural control, and achieve high utilization efficiency, good preventive effect, and improved seed quality.

Active Publication Date: 2019-05-03
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of low agricultural control efficiency and large chemical control pollution fo

Method used

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  • Biocontrol seed coating agent for tomatoes and application of biocontrol seed coating agent
  • Biocontrol seed coating agent for tomatoes and application of biocontrol seed coating agent
  • Biocontrol seed coating agent for tomatoes and application of biocontrol seed coating agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A kind of tomato biocontrol seed coating agent, comprises composite bacterial agent and auxiliary agent, and described composite bacterial agent is composed of Bacillus velezensis (Bacillus velezensis) WZ-37, Bacillus subtilis (Bacillus subtilis) WXCDD105, Pseudomonas nitrogen producing Bacillus azotoformans (Pseudomonas azotoformans) WXCDD51, Bacillus amyloliquefaciens Ba and Bacillus subtilis Bs wy-1.

Embodiment 2

[0051] A kind of tomato biocontrol seed coating agent, comprises composite bacterial agent and auxiliary agent, and described composite bacterial agent is composed of Bacillus velezensis (Bacillus velezensis) WZ-37, Bacillus subtilis (Bacillus subtilis) WXCDD105, Pseudomonas nitrogen producing Bacillus azotoformans (Pseudomonas azotoformans) WXCDD51, Bacillus amyloliquefaciens Ba and Bacillus subtilis Bs wy-1.

[0052] Among them, Bacillus velezensis WZ-37 has been preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.15766.

[0053] Bacillus Velez WZ-37 strain was drawn in three zones on LB plates, and after 24 hours of cultivation in a biochemical incubator at 37°C, the colonies were transparent, round, with moist, smooth surfaces and neat edges. After 48 hours of cultivation, the colonies were off-white. Round, dry surface, wrinkled, opaque, with neat edges, such as image 3 As shown i...

Embodiment 3

[0097] A kind of tomato biocontrol seed coating agent, comprises composite bacterial agent and auxiliary agent, and described composite bacterial agent is composed of Bacillus velezensis (Bacillus velezensis) WZ-37, Bacillus subtilis (Bacillus subtilis) WXCDD105, Pseudomonas nitrogen producing Bacillus azotoformans (Pseudomonas azotoformans) WXCDD51, Bacillus amyloliquefaciens Ba and Bacillus subtilis Bs wy-1.

[0098] Wherein the live bacteria amount of Bacillus Velez WZ-37 and Bacillus subtilis WXCDD105 in the composite bacterial agent is 10 6 cfu / mL, the viable counts of Pseudomonas azogenes WXCDD51, Bacillus amyloliquefaciens Ba and Bacillus subtilis Bs wy-1 were all 10 4 cfu / mL.

[0099] The additives in this embodiment include the following components by weight: 0.5-1.5 parts of thickener, 0.05-0.15 parts of dispersant, 0.5-1.5 parts of film-forming agent and 0.5-0.8 parts of coloring agent.

[0100] In this example, the volume-to-mass ratio of the composite microbial ...

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Abstract

The invention relates to a biocontrol seed coating agent for tomatoes and an application of the biocontrol seed coating agent, and belongs to the technical field of applications of biocontrol bacteria. In order to solve the problems of low efficiency of agricultural control and large pollution of chemical control aiming at a tomato damping-off disease and root rot, the invention provides the biocontrol seed coating agent for the tomatoes. The biocontrol seed coating agent comprises a composite microbial agent and auxiliary agents, wherein the composite microbial agent is formed by compoundingof a fermented liquid of bacillus velezensis WZ-37, a fermented liquid of bacillus subtilis WXCDD105, a fermented liquid of pseudomonas azotoformans WXCDD51, a fermented liquid of bacillus amyloliquefaciens Ba and a fermented liquid of bacillus subtilis Bs wy-1; and auxiliary agents comprise gibberellin, a thickening agent, a dispersant, a film forming agent and a coloring agent. The composite microbial agent provided by the invention has stable antibacterial effects on pathogens of the tomato damping-off disease and root rot, and the growth inhibition rates of hyphae of the two pathogenic bacteria both reach 100%; and the biocontrol seed coating agent is applied to prevent and control the tomato damping-off disease and root rot, and has the advantages of high efficiency, economy, safety and long longevity of residues.

Description

technical field [0001] The invention belongs to the technical field of application of biocontrol bacteria, in particular to a tomato biocontrol seed coating agent and its application. Background technique [0002] The two most common diseases in tomato seedling stage are tomato damping-off and root rot, which have brought huge economic losses to tomato production. The main manifestations of tomato damping-off disease are: damping-off and rotten seeds before emergence, a large number of water-soaked spots will grow on the stems near the soil in the early stage of planting, and the constriction of the stems in the later stage will lead to the death of tomato seedlings. When it is high, Pythium mycelium grows on the surface, that is, a white flocculent mold layer. Tomato root rot can occur regardless of seedlings or large seedlings, but the disease mainly occurs after planting. In the early stage of tomato root rot, the lateral roots gradually rot, and later the main root als...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N45/00A01N43/12A01P3/00A01P21/00A01C1/06C12N1/20C12R1/07C12R1/125C12R1/38
Inventor 王傲雪郑伟陈秀玲刘佳音张瑶
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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