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A kind of bursa active pentapeptide and application of promoting the immune response of aiv and/or ndv vaccine

A bursal polypeptide and bursa technology, applied in medical preparations containing active ingredients, peptides, antibody medical ingredients, etc., can solve the problem of consuming a lot of manpower and material resources, can not effectively protect the attack, and strengthen the stress response of chickens and other problems, to achieve the effect of improving antigen presentation response, promoting extensive immune enhancement, and improving vaccine immune efficacy.

Active Publication Date: 2022-04-22
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinical application shows that the existing H9N2 subtype AIV inactivated vaccine can not effectively protect chickens from the current H9N2 subtype AIV epidemic strains after immunizing chickens, although they can make chickens produce higher antibodies
Moreover, immunization with a single vaccine will consume a lot of manpower and material resources, and the increase in the number of immunizations will also strengthen the stress response of the chickens

Method used

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  • A kind of bursa active pentapeptide and application of promoting the immune response of aiv and/or ndv vaccine
  • A kind of bursa active pentapeptide and application of promoting the immune response of aiv and/or ndv vaccine
  • A kind of bursa active pentapeptide and application of promoting the immune response of aiv and/or ndv vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Isolation and identification of pentapeptide

[0035] Wash 50 g of AA broiler bursa tissues without fascia and adipose tissue once with 0.85% physiological saline (precooled to 4-10° C.). After draining, place the bursa tissue in a tissue masher, add pre-cooled saline, and homogenize at high speed three times, 30 seconds each time, and keep the homogenization process at 0-10°C. Then, the homogenate was ultrasonically lysed twice at 4°C, 5 min each time. Then the lysate was heated to 80°C, kept warm for 5min, then quickly placed on ice, and cooled to 10°C. The lysate was then refrigerated and centrifuged for 30 minutes (4000×g / min). The supernatant was collected and freeze-thawed twice. Then the supernatant was centrifuged at high speed at 12000g / min, 4°C, 30min. The supernatant was collected for ultrafiltration, and the ultrafiltrate with a molecular weight below 1000 Da was collected, that is, the crude extract of the bursa tissue. After freeze-drying, dilute w...

Embodiment 2

[0037] 1. Artificially synthesized pentapeptide

[0038] A commercial peptide synthesis company was entrusted to synthesize the peptide according to the MPPTH sequence (SEQ ID No.1), with a purity of 99.936%.

[0039] 2. Vaccines

[0040] Avian influenza and Newcastle disease dual inactivated vaccine (purchased from Nanjing Tianbang Biotechnology Co., Ltd.).

[0041] 3. Mouse immunization experiment

[0042] 3.1 Grouping of experimental animals and mice

[0043] The BALB / C mice were randomly divided into five groups, 10 in each group: (I) PBS control group (each immunized with 0.2ml PBS); (II) AIV+NDV double inactivated vaccine immunization group (each immunized with inactivated vaccine) live vaccine 0.2ml); (III~V) AIV+NDV double inactivated vaccine+pentapeptide group (pentapeptide concentrations were 10, 50, 250 μg / mL); intraperitoneal injection was used to treat the corresponding groups of mice respectively Immunized twice. The immunization interval was two weeks, 0.2m...

Embodiment 3

[0051] 1. Artificially synthesized pentapeptide

[0052] A commercial peptide synthesis company was entrusted to synthesize the peptide according to the MPPTH sequence (SEQ ID No.1), with a purity of 99.936%.

[0053] 2. Vaccines

[0054] Avian influenza and Newcastle disease dual inactivated vaccine (purchased from Nanjing Tianbang Biotechnology Co., Ltd.).

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Abstract

The invention relates to a bursa active pentapeptide for promoting the immune response of AIV and / or NDV vaccines and its application. The bursa active pentapeptide of the present invention has the amino acid composition of Met Pro Pro Thr His, simple structure and extremely weak immunogenicity. The active peptide of the present invention has the function of promoting vaccine immune response, and in the mouse immunization experiment, it not only promotes the generation of AIV-specific antibody response in immunized mice, but also promotes the generation of NDV-specific antibody response in immunized mice. In addition, it also promotes cellular immune response, antigen presentation response, and improves the vaccine immune efficacy of immunized chickens. It can be used as a vaccine adjuvant or immune enhancer in animal vaccine application research to improve the animal body’s immune response to specific antigens. Improve the immune efficacy of the vaccine, thereby improving the ability of the animal body to resist epidemic infections.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a bursa active pentapeptide capable of promoting the immune response of vaccine AIV and / or double vaccine with NDV and application thereof. Background technique [0002] Avian influenza (AI) is a highly contagious disease of poultry caused by Avian influenza virus (AIV) of the Orthomyxoviridae family. For egg production drop and respiratory disease, even lead to systemic disease, the mortality rate can reach 100%. According to the difference of pathogenicity, it can be divided into highly pathogenic avian influenza virus and low pathogenic avian influenza virus. At present, the low-pathogenic avian influenza epidemic in China is mainly H9 subtype. Although this subtype of avian influenza virus does not cause a large number of deaths of infected poultry, due to its strong transmission ability and wide range of existence, it leads to decreased e...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K39/39A61K39/145A61K39/17A61P31/14A61P31/16
Inventor 冯秀丽宗嫚嫚郝珊珊郑阳张则蔡佳希曹瑞兵陈溥言
Owner NANJING AGRICULTURAL UNIVERSITY
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