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Gold nanocluster using transferrin as template, and preparation method and application thereof

A technology of gold nanoclusters and transferrin, which is applied in the field of red fluorescent gold nanoclusters and its preparation, can solve the problems of harsh reaction conditions and cytotoxicity of quantum dots, and achieve short reaction time, good biocompatibility, and proportional low effect

Active Publication Date: 2019-05-03
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Unfortunately, heavy metal-containing QDs are harsh and potentially cytotoxic

Method used

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  • Gold nanocluster using transferrin as template, and preparation method and application thereof
  • Gold nanocluster using transferrin as template, and preparation method and application thereof
  • Gold nanocluster using transferrin as template, and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Transferrin was reduced and coated to prepare red fluorescent gold nanoclusters.

[0031] Weigh 5mg of transferrin (Tf), add 1mL distilled water to it, and make 5mg / mL transferrin solution. Weigh 206.0mg tetrachloroauric acid (HAuCl 4 4H 2 O), prepare the HAuCl of 0.01M concentration in the 50mL brown volumetric flask 4 solution and refrigerated at 4°C for later use. Prepare 10 mL of 1M NaOH solution. Take 1 mL of 0.01M HAuCl 4 The solution was diluted four times with distilled water. Take 1 mL of the diluted solution, put it into a microwave tube, add 1 mL of transferrin solution and stir at room temperature for 5 minutes. After adjusting the pH of the mixed solution to 12 with 40 μL of 1 M NaOH solution, the solution was placed in a microwave reactor and sealed. The reaction conditions were set as 80° C., 60 min, 150 W, 250 psi, high stirring speed, and the pre-stirring time was 2 min. After the reaction, it was placed in a 4°C refrigerator for later use ( figu...

Embodiment 2

[0033] (1) UV and fluorescence characterization of gold nanocluster AuNCs@Tf.

[0034] figure 2 As observed in the inset of A, the as-synthesized material appears brown under fluorescent light and has strong red fluorescence under UV light. Take 200 μL of the prepared AuNCs@Tf solution and add 800 μL distilled water for dilution, and measure the UV absorption curve and fluorescence emission curve. The results of ultraviolet absorption show that there is obvious absorption at 280nm, and the fluorescence spectrum shows that the best emission peak is at 640nm.

[0035] (2) Infrared analysis (FTIR) spectrum characterization of gold nanocluster AuNCs@Tf.

[0036] In order to confirm the binding of transferrin on the surface of AuNCs@Tf, the prepared AuNCs@Tf liquid was dried by a freeze dryer to obtain a solid. Take transferrin and potassium bromide at a mass ratio of 1:100, mix and grind them into tablets, and measure their infrared spectra; then take the prepared AuNCs@Tf and...

Embodiment 3

[0048] Fluorescence study of the interaction of various ions with the synthesized gold nanocluster AuNCs@Tf.

[0049] Prepare 2 mL of each ion solution of 0.1 M (Mg 2+ ,Pb 2+ , K + ,Cd 2+ ,Cr 3+ , Bi 3+ ,Zn 2+ , Na + ,Al 3+ , Ca 2+ , Ag + ,Mn 2+ , Ba 2+ ,Cu 2+ ,Hg 2+ ,Co 2+ , Ni 2+ ) In a 2 mL EP tube, take 1 mL of the prepared AuNCs@Tf solution, add 9 mL of Tris-HCl solution with pH=4.5 to it for dilution. Set the parameters of the fluorescence spectrometer (λ ex =410nm,λ em =570nm-740nm), take 1mL and place it in a fluorescent cuvette to scan the sample and record the data; add 10μL Mg 2+ After stirring the solution, time it for 2 minutes, scan the sample, record the data, and repeat the above experiment for other ions. image 3 A recorded the experimental results, which proved that Cu 2+ It can quench the fluorescence of AuNCs@Tf.

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Abstract

The invention provides a gold nanocluster using transferrin as template, and a preparation method and application thereof. The preparation method of the nanoclusters comprises the following steps: respectively configuring aqueous solutions of HAuCl4 and Tf, mixing them according to the molar ratio of 20-60:1, stirring for 2-5 minutes at room temperature, adjusting the pH of the mixed solution to 12 with NaOH solution, placing the solution in a microwave reactor and sealing, reacting at 70-90 DEG C for at least 50 minitues to obtain AuNCs@Tf emitting intense red fluorescence. The nanoclusters of the invention can be used for detecting copper ions, and can be applied to a copper ion detection test paper to visualize the detection of copper ions. The nanoclusters of the invention can also beused for detecting glutathione, and the cell experiment shows that the nanoclusters can target lysosomes to recognize cancer cells. The nanoclusters of the invention can also be used for encrypting and decrypting fingerprint information.

Description

technical field [0001] The invention relates to a fluorescent detection material, in particular to a red fluorescent gold nanocluster with transferrin as a template, a preparation method and application thereof. Background technique [0002] Copper ions (Cu 2+ ) is one of the trace elements in humans and other mammals and plays a key role in our physical health. Cu 2+ Abnormal ingestion of carbs can cause intestinal disturbances and liver or kidney damage as well as several neurodegenerative diseases such as Alzheimer's, Parkinson's and Wilson's diseases. In addition, excessive industrial emissions will also cause environmental pollution. Therefore, finding a sensitive method for detecting copper ions is of great value for the detection of copper ion pollution in food and environment. [0003] Glutathione (GSH) is a tripeptide containing a sulfhydryl group and a γ-amide bond, which plays a key role in many physiological and pathological processes. Essentially every cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/58B82Y20/00B82Y30/00G01N21/64
Inventor 李英奇赵鹤妙
Owner SHANXI UNIV
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