Production technology of granular threonine

A production process, technology of threonine, applied to the preparation of microorganisms, organic compounds, methods based on microorganisms, etc., can solve problems such as increased fermentation costs, difficulties in separating products, and difficult control of fermentation processes, so as to improve quality and added value , Control solubility, strengthen the effect of CO2 fixation reaction

Active Publication Date: 2019-05-03
齐齐哈尔龙江阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mixed fermentation process of strains is difficult to control, the cost of fermentation is increased, and there are certain difficulties in the separation of products in the later stage.

Method used

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  • Production technology of granular threonine
  • Production technology of granular threonine
  • Production technology of granular threonine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The production technology of granular threonine, it comprises the steps:

[0030] Step 1) Preparation of fermentation medium: glucose 25g / L, glycerin 20g / L, corn steep liquor 20g / L, ammonium sulfate 2g / L, potassium dihydrogen phosphate 0.1g / L, dipotassium hydrogen phosphate 0.1g / L, heptahydrate Magnesium sulfate 0.1g / L, ferrous sulfate heptahydrate 5mg / L, manganese sulfate monohydrate 5mg / L, isoleucine 200mg / L, fulvic acid 10mg / L, pH 5.5;

[0031] Step 2) Fermentation: Escherichia coli engineering bacteria K12△dapA seed solution (the concentration of the seed solution is 1×10 8 cfu / mL) according to 12% of the inoculum amount was inserted into the fermenter containing the fermentation medium for fermentation, the temperature was 30°C, the tank pressure was 0.03MPa, the ventilation rate was 0.4vvm, the rotation speed was 100rpm, and the fermentation time was 36h, and then Add inositol 100mg / L, continue fermentation and cultivation for 24 hours, control the sugar content ...

Embodiment 2

[0035] The production technology of granular threonine, it comprises the steps:

[0036] Step 1) Preparation of fermentation medium: glucose 30g / L, glycerol 15g / L, corn steep liquor 15g / L, ammonium sulfate 2g / L, potassium dihydrogen phosphate 0.2g / L, dipotassium hydrogen phosphate 0.2g / L, heptahydrate Magnesium sulfate 0.2g / L, ferrous sulfate heptahydrate 10mg / L, manganese sulfate monohydrate 10mg / L, isoleucine 300mg / L, fulvic acid 20mg / L, pH 5.5;

[0037] Step 2) Fermentation: Escherichia coli engineering bacteria K12△dapA seed solution (the concentration of the seed solution is 1×10 8 cfu / mL) according to the inoculum amount of 10-12%, put it into the fermenter containing the fermentation medium for fermentation, the temperature is 30°C, the tank pressure is 0.04MPa, the ventilation volume is 0.6vvm, the rotation speed is 100rpm, and the fermentation time is 32h , then add inositol 200mg / L, continue fermentation and cultivation for 28h, control the sugar content to 0.4% by ...

Embodiment 3

[0041] The influence of different factors in the fermentation process of the present invention on the yield of threonine, the content of by-products such as acetic acid and lactic acid.

[0042] Set group:

[0043] Experimental group: Example 1;

[0044] Control group 1: No addition of isoleucine, the rest are the same as in Example 1;

[0045] Control group 2: do not add fulvic acid, all the other are the same as embodiment 1;

[0046] Control group 3: without adding inositol, the rest are the same as in Example 1;

[0047] Control group 4: Glycerin was replaced by glucose of the same quality, and the rest were the same as in Example 1.

[0048] The contents of threonine, acetic acid and lactic acid in the final fermentation liquid of each group were as follows: Figure 1-3 shown. By setting up a control group, the effects of fermentation factors such as glycerin, fulvic acid, isoleucine and inositol on threonine, acetic acid and lactic acid were detected; Figure 1-3 I...

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Abstract

The invention belongs to the technical field of the production of amino acids, and discloses a production technology of granular threonine. The production technology comprises the following steps of step (1) preparing fermentation mediums; step (2) performing fermentation; step (3) performing centrifugation; and step (4) preparing the granular threonine. The threonine is high in yield and few in by-products.

Description

technical field [0001] The invention belongs to the field of amino acid production, and in particular relates to a production process of granular threonine. Background technique [0002] Threonine is easily soluble in water but not in organic solvents such as ethanol and ether. It is an aliphatic amino acid with a slightly sweet taste. It is an essential amino acid that constitutes human, animal and plant proteins. Threonine, as one of the essential amino acids for the human body, plays an increasingly important role in people's lives. With the development of the breeding industry and the rapid increase in the demand for livestock and poultry feed, more and more attention has been paid to the role of threonine as a nutrient that must be ingested from the outside. It is widely used in medicine, food and feed. Threonine is one of the products of industrial fermentation. According to statistics, the global supply of threonine reached 685,000 tons in 2017, a year-on-year incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08C07C227/40C07C229/22C12R1/19
Inventor 刘海涛董力青李航汲广习王铮
Owner 齐齐哈尔龙江阜丰生物科技有限公司
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