Qualitative and quantitative detection method of one or more of cannabidiol, cannabidiolic acid and tetrahydrocannabinol
A quantitative detection method, the technology of cannabidiolic acid, applied in the detection field, can solve the problems of peak time and peak shape influence, inability to detect content in real time, inability to separate signal peaks, etc., improve extraction efficiency, realize real-time quantitative transformation, research-friendly effect
Inactive Publication Date: 2019-05-07
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI +1
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Problems solved by technology
[0004] Due to the conversion relationship between cannabidiolic acid and cannabidiol, the existing detection conditions cannot completely separate and non-overlap the signal peaks of cannabidiolic acid and cannabidiol, and cannot detect their specific content in real time
There are many problems in the detection of the above three substances by high performance liquid chromatography, such as the choice of detection wavelength, the ratio of mobile phase, and the choice of solvent all have a major impact on the peak time and peak shape, thus affecting quantitative determination
At present, there is no method for separating the three substances of cannabidiolic acid, cannabidiol and tetrahydrocannabinol in cannabis at the same time
Method used
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Embodiment 1
[0052] 1. Take 0.2 g of crushed cannabis flower leaves, use 4 mL of methanol with a material-to-liquid ratio of 1:20, and use room temperature ultrasonic extraction for 10 minutes, and let stand for one hour.
[0053] 2. Centrifuge at 4000r / min for 5 minutes in a centrifuge, and transfer the supernatant to a 5mL volumetric flask for constant volume.
[0054] 3. Use a 2mL syringe to fill the sample through a 0.45μm organic filter membrane and inject it into the liquid phase injection bottle.
[0055]4. The high-performance liquid phase setting condition is that the injection volume is 10 μL. The chromatographic column is Waters Xbridge, 250mm×4.6mmi.d., 5μm. The mobile phase was 0.1% acetic acid and acetonitrile (25:75), the flow rate was 0.8 mL / min, the column temperature was 25° C., and the wavelength was 220 nm.
[0056] 5. Draw the standard curve.
[0057] Prepare a methanol solution of cannabidiolic acid with a concentration of 1 mg / mL, measure 50 μL, 100 μL, 200 μL, 30...
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Abstract
The invention relates to the technical field of detection, in particular to a qualitative and quantitative detection method of one or more of cannabidiol, cannabidiolic acid and tetrahydrocannabinol.The qualitative and quantitative detection method of one or more of the cannabidiol, the cannabidiolic acid and the tetrahydrocannabinol comprises the following steps that: ultrasound extraction is conducted on smashed hemp flower leaves through methyl alcohol, and a test solution is obtained; the test solution is separated and detected through high performance liquid chromatography, a moving phase of the high performance liquid chromatography is a mixture of acetic acid water solution and acetonitrile, and isocratic elution is conducted; and the content of one or more of the cannabidiol, thecannabidiolic acid and the tetrahydrocannabinol are detected through an external standard method. According to the qualitative and quantitative detection method of one or more of the cannabidiol, thecannabidiolic acid and the tetrahydrocannabinol, specific wave length and a moving phase are selected, the peak pattern is good, sensitivity is high, research is conveniently conducted, and qualitative and quantitative treatment of the cannabidiol, the cannabidiolic acid and the tetrahydrocannabinol can be detected quickly and accurately under treatment of the same sample.
Description
technical field [0001] The invention relates to the technical field of detection, in particular to a qualitative and quantitative detection method for one or more substances in cannabidiol, cannabidiolic acid and tetrahydrocannabinol. Background technique [0002] Cannabis (scientific name: Cannabis sativa L.) is a plant of the genus Cannabis in the family Moraceae, an annual erect herb. Cannabis was originally distributed in India, Bhutan and Central Asia, and now it is wild or cultivated in various countries. The fruit is called "huomaren" or "cannabinoids" in traditional Chinese medicine. It is flat in nature and sweet in taste. The flower is called "Mabo", and it is mainly used to cure bad wind, amenorrhea, and forgetfulness. The husk and bracts are called "hemp", which is poisonous and can be used to treat overworked injuries, break up accumulation, and disperse pus. Taking too much will make you mad; the leaves contain anesthetic resin and can be used to prepare anes...
Claims
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IPC IPC(8): G01N30/02G01N30/06
Inventor 赵立宁严江涛常丽张江江李德芳刘亮亮
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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