Method for determining 19 pesticide residues in alfalfa by liquid chromatography-tandem mass spectrometry
A technology of tandem mass spectrometry and liquid chromatography, applied in the field of determination of 19 kinds of pesticide residues in alfalfa by liquid chromatography-tandem mass spectrometry, can solve problems affecting quality and human quality threats
Active Publication Date: 2019-05-10
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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[0014] However, according to the research of the prior art, it is found that the determination of pesticide residues in alfalfa is very little. The quality of meat, eggs, and milk poses a threat to human body quality, so it is urgent to develop a fast and accurate method for the detection of pesticide residues in alfalfa
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[0091] 1. Experimental part
[0092] 1.1 Instruments and reagents
[0093] API4000 liquid chromatography tandem mass spectrometer (SCIEX, USA), centrifuge (Hitachi, Japan), vortex mixer (Scilogex, USA), analytical balance (Sartorius, Germany).
[0094] 19 kinds of pesticide standard products (diuron, secbutaline, malathion, dimethoate, carbaryl, imidophos, oxidized carboxyl, triadimefon, diniconazole, metalaxyl, methyl Thiophanate, azoxystrobin, carbendazim, imidacloprid, diazinon, isofenphos-methyl, isoprocarb, methiocarb, phoxim) were purchased from Dr. Ehrenstorfer in Germany and Sigma-aldrich in the United States (purity 99.0 %); Acetonitrile is chromatographically pure (Sigma Aldrich, U.S.); Formic acid is chromatographically pure (Dikma Pure, U.S.); Laboratory water is ultrapure water; Homoproton (Tianjin Agela Technologies); QuEChERS extraction salt bag is QuEChERS extraction tube (50ml, Containing 6g of anhydrous magnesium sulfate and 1.5g of anhydrous sodium acetate...
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Abstract
The invention discloses a method for determining 19 pesticide residues in alfalfa by liquid chromatography-tandem mass spectrometry, belonging to the technical field of detection methods. The method for determining the 19 pesticide residues in alfalfa by the liquid chromatography-tandem mass spectrometry comprises the following steps: (1) performing sample pretreatment; and (2) determining the 19pesticide residues in a pretreated alfalfa sample through the liquid chromatography-tandem mass spectrometry. In the method for determining the 19 pesticide residues in alfalfa by the liquid chromatography-tandem mass spectrometry, acetonitrile is used as an extraction solvent, extraction is carried out through a QuEChERS extraction tube, purification is carried out through a QuEChERS purificationtube, and the MRM monitoring analysis is carried out by means of the LC-MS / MS, which is simple in operation and can significantly improve the work efficiency; and the method for determining the 19 pesticide residues in alfalfa by the liquid chromatography-tandem mass spectrometry also has the advantages of high accuracy and sensitivity, low detection limit and good linearity.
Description
technical field [0001] The invention relates to a method for detecting multiple pesticide residues in alfalfa, in particular to a method for determining 19 kinds of pesticide residues in alfalfa by liquid chromatography-tandem mass spectrometry. Background technique [0002] Alfalfa provides a relatively stable and suitable living environment for insects due to its biological characteristics such as perennial, lush branches and leaves, and high nutritional value. It is known as an insect resource bank because of its rich species of pests and natural enemies. Among them, the types of pests mainly include aphids, thrips, and mirid bugs, which pose a serious threat to the production of alfalfa. Chemical pesticide control of pest damage is still one of the important methods used in agriculture, so there is a certain amount of drug residues on alfalfa. , such as carbaryl, imophos, oxidized carboxyl, thiophanate-methyl, diazinon, isofenphos-methyl, isoprocarb, phoxim, etc. [000...
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IPC IPC(8): G01N30/88
Inventor 谷旭秦玉昌高云峰李军国李俊杨晓伟杨洁刘钧
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES