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Preparation method for collagen hydrogel

A technology of collagen and hydrogel, applied in the field of preparation of collagen hydrogel, to achieve uniform state, easy process, and increase the effect of reaction rate

Active Publication Date: 2019-05-17
HEBEI COLLAGEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the precipitation of a certain amount of liquid in the sample after the irradiation of the collagen hydrogel, the purpose of the present invention is to provide a preparation method of the collagen hydrogel, which forms a hydrogel with a uniform state, and solves the problem of a certain amount of liquid after irradiation. liquid precipitation problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Collagen solution preparation: Weigh 150g of collagen, put it in a 50L stirring reaction kettle, add 30kg of acetic acid solution with a concentration of 0.05mol / L, and make the solution repeatedly pass through a 100-mesh screen in a peristaltic pump to achieve a uniform state.

[0022] (2) Adding amino acid: Add 0.15 g of lysine powder to the collagen solution prepared in step (1), and continue to make the solution repeatedly pass through a 100-mesh sieve in a peristaltic pump to achieve a uniform state.

[0023] (3) pH adjustment: place the solution prepared in step (2) at a low temperature of 4° C., perform ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 30 kDa, and add a phosphate buffer of pH 6.0 during the ultrafiltration process, Continuous ultrafiltration until the difference between the pH of the permeate and 6.0 is less than 0.2.

[0024] (4) Irradiation: subpackage the sample prepared in step (3) and irradiate it with a...

Embodiment 2

[0027] (1) Collagen solution preparation: Weigh 50g of collagen, place it in a 50L stirring reaction kettle, add 5kg of 0.1mol / L butyric acid solution, and repeatedly pass the solution through a 400-mesh screen in a peristaltic pump to achieve a uniform state.

[0028] (2) Adding amino acid: Add 2.5 g of glutamic acid powder to the collagen solution prepared in step (1), and continue to pass the solution repeatedly through a 400-mesh sieve in a peristaltic pump to achieve a uniform state.

[0029] (3) pH adjustment: the solution prepared in step (2) is placed in a low temperature of 0°C, and ultrafiltration is performed using an ultrafiltration membrane with a molecular weight cut-off of 1kDa, and ultrapure water of pH 7.0 is added during the ultrafiltration process, continuously Ultrafiltration until the difference between the pH of the permeate and 7.0 is less than 0.2.

[0030] (4) Irradiation: subpackage the sample prepared in step (3) and irradiate it with an electron bea...

Embodiment 3

[0033] (1) Collagen solution preparation: Weigh 100g of collagen, put it in a 50L stirring reaction kettle, add 16kg of 0.3mol / L propionic acid solution, and repeatedly pass the solution through a 200-mesh screen in a peristaltic pump to achieve a uniform state.

[0034] (2) Adding amino acids: Add 0.7 g of arginine and 0.3 g of aspartic acid powder to the collagen solution prepared in step (1), and continue to pass the solution repeatedly through a 200-mesh sieve in a peristaltic pump to achieve a uniform state.

[0035] (3) pH adjustment: place the solution prepared in step (2) at a low temperature of 8° C., perform ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 10 kDa, and add a phosphate buffer solution of pH 7.4 during the ultrafiltration process, Continuous ultrafiltration until the difference between the pH of the permeate and 7.4 is less than 0.2.

[0036] (4) Irradiation: the sample prepared in step (3) was subpackaged and then irr...

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Abstract

The invention discloses a preparation method for collagen hydrogel. The method comprises the following steps that (1) a collagen solution is prepared, specifically, a certain amount of collagen is weighed and put into a reaction kettle, a 0.05-0.5 mol / L organic acid solution with 40-200 times the weight of the collagen is added, and the solution is enabled to pass through a 50-400-mesh sieve repeatedly under the action of a peristaltic pump so as to reach a uniform state; (2) amino acid is added, specifically a certain amount of amino acid is added into the collagen solution prepared in the step (1), and the solution is continuously enabled to pass through a 50-400-mesh sieve repeatedly under the action of the peristaltic pump so as to reach a uniform state; (3) the pH is adjusted, specifically, the solution prepared in the step (2) is placed at a low temperature of 0-10 DEG C, solution replacement is carried out by adopting an ultrafiltration method, ultra-pure water or a buffer solution with the pH value of 6-8 is added in the ultrafiltration process, and ultrafiltration is continuously carried out until the difference value between the pH of permeate liquid and the pH of the added ultrapure water or the buffer solution is less than 0.2; and (4) irradiating is carried out, specifically, a sample prepared in the step (3) is subjected to split packaging and then is irradiated.The preparation method for the collagen hydrogel has the advantages that the uniform hydrogel can be prepared, and the problem of liquid precipitation after irradiation can be solved.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular, the invention relates to a preparation method of collagen hydrogel. Background technique [0002] Collagen is a natural biopolymer with a triple helix structure and is the most abundant protein in animals. About 30% of all proteins in mammals are collagen. Collagen is widely distributed in various tissues and organs of the human body, with the highest content in connective tissue. Because it is the main component of various tissues in the body, it acts as a scaffold for cells and tissue structures, and has an important impact on the morphology, metabolism, growth and differentiation of cells and tissues. Collagen is divided into many types according to the difference in amino acid composition of each chain. Although the structures of various collagens are very different, they have a common feature: they are all composed of three α-peptide chains in a right-handed helical manner...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/28C08L89/00
Inventor 赵磊磊王云松
Owner HEBEI COLLAGEN BIOTECH
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