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A kind of Paenibacillus dendriticum, immobilized bacterial agent and application thereof

A Bacillus and dendritic technology, applied in the field of Paenibacillus dendriticum and immobilized inoculants, can solve the problems of high cost and low removal efficiency, and achieve the effect of facilitating application, maintaining the concentration of viable bacteria and increasing the rate.

Active Publication Date: 2020-07-17
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of high cost and low removal efficiency in existing antibiotic pollution treatment methods, the invention provides a Paenibacillus dendriticum

Method used

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  • A kind of Paenibacillus dendriticum, immobilized bacterial agent and application thereof
  • A kind of Paenibacillus dendriticum, immobilized bacterial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The Bacillus arborescens with the deposit number CGMCC No. 14473 was inoculated into the beef extract peptone culture solution from the preservation slope, and cultured with shaking at 37° C. and 180 rpm for 12 hours to prepare the seed solution.

[0055] 1) Inoculate Bacillus arborescens (TF1) with the preservation number of CGMCC No.14473 in beef extract peptone liquid medium, and cultivate to log phase at 37°C;

[0056] 2) Centrifuge the bacterial solution obtained in step 1) for 10 minutes at 5000 rpm to collect the bacterial cells, wash the bacterial cell pellet with sterile water, and repeat twice to obtain wet bacterial cells;

[0057] 3) Mix the bacterial sludge obtained in step 2) with activated carbon and adsorb for 8 minutes;

[0058] 4) Heat the polyvinyl alcohol and sodium alginate aqueous solution to form a gel separately, mix the sodium alginate gel and polyvinyl alcohol gel with a weight content ratio of 1:43.33, and then mix the mixed gel with the activated carb...

Embodiment 2

[0063] Using the seed solution of Example 1, the bacteria solution was aseptically filtered and freeze-dried to obtain the microbial inoculum. In each gram of the microbial inoculum, the number of viable bacteria was 10 10 ~10 11 cfu.

Embodiment 3

[0065] Using the method and material of Example 1, the difference is that in step (3), the sodium alginate gel and the polyvinyl alcohol gel are mixed at a weight content ratio of 1:60, and then the mixed gel and the activity of the adsorbing bacterial mud are mixed Mix the carbon thoroughly. Per gram of the immobilized microspheres of this example, the number of viable Bacillus dendriticum with the deposit number CGMCC No. 14473 is 10 8 ~10 9 cfu.

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Abstract

The invention relates to paenibacillus dendritiformis, an immobilized microbial agent and application thereof. The preservation number of the paenibacillus dendritiformis is CGMCC No. 14473. The invention also provides the immobilized microbial agent containing the paenibacillus dendritiformis as described above as an active ingredient. The invention provides the application of the above-describedpaenibacillus dendritiformis and the immobilized microbial agent for degrading oxytetracycline and repairing oxytetracycline-containing water body, soil and poultry farming waste. Through the above technical scheme, the microbial agent more effectively removes the oxytetracycline, is especially capable of rapidly and efficiently removing the oxytetracycline in the water body containing the oxytetracycline, and reducing residues, and has the advantages of convenient application and separation and reusability.

Description

Technical field [0001] The present disclosure relates to a dendritic bacillus, an immobilized bacterial agent and applications thereof. Background technique [0002] Tetracycline antibiotics are currently one of the most widely used antibiotics in the world, accounting for more than 50% of the antibiotic consumption in livestock and aquaculture industries. Among them, Oxytetracycline (OTC) is widely used in livestock, poultry and aquaculture as a therapeutic drug and feed additive due to its wide antibacterial spectrum and low price. The mechanism of action of oxytetracycline is to interfere with protein synthesis. Its side effects are small, and it is mainly used for respiratory and intestinal infections. As a feed additive, oxytetracycline with good antibacterial effect can effectively inhibit harmful microorganisms in the digestive tract of livestock and poultry, increase the survival rate of livestock and poultry, enhance the appetite of livestock and poultry, improve feed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/084C02F3/34B09C1/10A62D3/02C12R1/01A62D101/26A62D101/28
Inventor 李红娜田云龙朱昌雄李峰叶婧耿兵郭萍李莲芳
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI