Cell carrier particle aggregate and preparation method thereof

A technology of carrier particles and aggregates, applied in general culture methods, biochemical equipment and methods, fixed on/in organic carriers, etc., can solve problems such as difficulty in use, reduction of effective mass, and splashing of microcarrier powder. Achieve beautiful and convenient use, facilitate aseptic operation, and avoid static electricity

Active Publication Date: 2019-05-17
BEIJING CYTONICHE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because it is a micron-level microbead, there is a lot of static electricity in the dry powder state, so it is very easy to cause the splash of the microcarrier powder during use or transportation, the effective mass is reduced, and the surrounding environment is polluted at the same time.
It is also difficu

Method used

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  • Cell carrier particle aggregate and preparation method thereof
  • Cell carrier particle aggregate and preparation method thereof
  • Cell carrier particle aggregate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0064] Example 1. Stamping and gathering forming

[0065] The stamping and forming machine is an automatic stamping and forming machine, the motor rocker rotates once to produce a piece of finished product. The stamping aggregation forming machine adopts the volumetric method to fix and quantify the volume.

[0066] First, weigh 20 mg of cell carrier particles on a balance, then pour the weighed material into the feed of the punching machine, and select the inclined flat punching mold. Adjust the upper punch of the stamping aggregation forming machine with an adjustment range of 0-50mm, and adjust the lower punch of the stamping aggregation forming machine with an adjustment range of 0-50mm. Run the handle of the stamping aggregation forming machine to fill the material, adjust the pressure value, the pressure value can be controlled within the range of 0~200KN, turn on the motor, the motor rocker shakes a week to complete the stamping aggregation forming. The automatic stamping ...

Example Embodiment

[0077] Example 2. Molding

[0078] Take a certain quality of powdered cell carrier particles, add deionized water (additional amount is 50 times the weight of the cell microcarrier), and use auxiliary tools to make it evenly and evenly spread the mold. The mold is cylindrical (can also be round, Square, rhombus, triangle, ellipse, polygon and polygon, etc.). Put a mold containing a certain quality of cell carrier particles (such as Figure 8 Shown) Put it in an oven at 60°C and continue drying for 96 hours to obtain a solid and stable cell carrier particle aggregate with the same unit mass, the same shape, as Picture 9 Shown.

[0079] Putting the cell carrier particle aggregate prepared in this example into water can quickly disperse into the state of individual cell carrier particles, such as Picture 10 As shown, the results show that its appearance and physical properties have not changed in any way.

[0080] The cell carrier particle aggregate prepared in this example has the p...

Example Embodiment

[0083] Example 3: Freeze-drying and drying

[0084] Take a certain quality of powdered microcarriers, add deionized water (additional amount is 100 times the weight of the cell microcarriers), and use auxiliary tools to make them aggregate into regular shaped blocks, such as Picture 11 As shown; put the aggregates in a freezer, and in the process of freezing at a low temperature of 0~-196℃, a block-shaped frozen body is formed; put the frozen aggregates in a freeze dryer, freeze-dry for 96 hours, and take out Then a freeze-dried aggregate is formed, such as Picture 12 Shown.

[0085] Put the cell carrier particle aggregate prepared in this example into water, and it can be quickly dispersed into the state of individual microcarrier particles, such as Figure 13 As shown, the results show that its appearance and physical properties have not changed in any way.

[0086] The cell carrier particle aggregate prepared in this example has the property of quickly absorbing water and disper...

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Abstract

The invention discloses a cell carrier particle aggregate and a preparation method thereof. The cell carrier particle aggregate is formed by aggregating cell carrier particles and has specific shapes;the specific shapes include a sheet shape and a block shape. The cell carrier particle aggregate has excellent rehydration property and good dispersibility after rehydration. The cell carrier particles in the cell carrier particle aggregate have porous connectivity inside, and have great elasticity and extrusion resistance. After the aggregate is prepared by pressing, a very small amount of liquid carrier particles can be quickly dispersed into a single carrier stent without any change in physicochemical properties; no other component is added, powder does not exist on the surface of the aggregate obtained by the forming method, so that the static electricity of the aggregate is reduced as much as possible, and the loss caused by the static electricity of a powder product is avoided. Besides, single-piece quantitative sterilization storage in the form of a medicine plate can be achieved, the aggregate can also be quantitatively sterilized and stored in multiple pieces in a bottled form, independent packaging and sterilization are achieved, and aseptic operation of cell culture is facilitated.

Description

technical field [0001] The invention relates to a cell carrier particle aggregate and a preparation method thereof. Background technique [0002] Cell carrier particles (generally 1-1000um in diameter) refer to microbeads that can be grown or co-cultured with adherent cells. At present, microcarriers on the market are used, stored and packaged in the form of powder. Because it is a micron-level microbead, it is easy to have a lot of static electricity in the dry powder state, so it is very easy to cause the splash of the microcarrier powder during use or transportation, reduce the effective mass, and pollute the surrounding environment while also polluting itself. Preservation of powdered microcarriers is also relatively difficult, and its nature limits that it will cause a lot of unnecessary loss no matter what packaging is used. It is difficult to use, and a lot of measuring equipment is needed as auxiliary tools in many processes such as weighing, which is time-consumin...

Claims

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Application Information

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IPC IPC(8): C12N11/00
CPCB29B11/06C12N5/0075C12N2533/52C12N2533/54C12M25/16B29C41/003B29C41/38B29K2089/00B29K2833/12C12N11/02B29C43/003B29C43/36B29C39/003B29C39/02B29C39/26C08J3/12B29C2043/3615
Inventor 刘伟鄢晓君
Owner BEIJING CYTONICHE BIOTECH CO LTD
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