Method for preparing low-energy structural grease by enzymatic transesterification

Abstract

The invention discloses a method for preparing low-energy structural grease by enzymatic transesterification, and belongs to the field of preparation of novel structural grease. The preparation methodcomprises the steps of putting corn oil and triacetin into a rubber tube in a certain ratio, conducting airtight shock reaction under certain conditions, filtering out lipase, evaporating a product in the rotating mode and storing the evaporated product at -20 DEG C in the sealing mode for use. Through a single factor test, the optimal substrate molar ratio, the enzyme addition amount, the reaction temperature and the reaction time for preparing the structural grease are determined. The prepared structural grease is separated and purified by a molecular distillation method. The optimum evaporation temperature, scraper speed and feed rate are finally determined by the single factor test.

Description

technical field [0001] The invention relates to a method for preparing low-energy structural lipids by enzymatic transesterification, which belongs to the field of preparation of new structural lipids, and uses molecular distillation to separate and purify them, and determines the method for preparing structured lipids through high-performance liquid chromatography analysis. Optimum process conditions. Background technique [0002] Fat is an important component of food and diet. It is a good source of fat-soluble bioactive compounds, assists the digestion and absorption of fat-soluble substances, and can give food good sensory properties. It can provide about one-fold higher energy in metabolism than sugar and protein. times. Corn oil is a common edible oil in our life. It is rich in vitamin E and unsaturated fatty acids necessary for the human body, with a content of more than 80%, mainly linoleic acid and oleic acid, of which linoleic acid accounts for more than 50% of th...

AI Technical Summary

Problems solved by technology

At present, the preparation of structural lipids includes physical methods, chemical methods and enzymatic synthesis. The physical method is to fractionate natural oils or simply blend different oils. The distribution of fatty acids in triglycerides has not changed and cannot To meet higher nutritional requirements, chemical methods are prone to produce by-products and the distribution of fatty acids on glycerol cannot be controlled. Enzymatic synthesis has high selectivity, little or no by-products, and no pollution to the environment. It is a green Sustainable synthesis method is of great significance for future large-scale production

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