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Green pigment of pumpkin seed and extraction method thereof

An extraction method, the technology of pumpkin seeds, which is applied in the field of natural pigment extraction, can solve the problems of shortening the extraction time, achieve the effects of no toxic side effects, reduce environmental pollution, and pure color

Inactive Publication Date: 2019-05-21
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Pulsed Electric Field (Pulsed Electric Field, PEF) is a non-thermal treatment technique that consists of repeated short pulses of high voltage pulses of a biological material (plant, animal or microbial cells) placed between two electrodes, a process that enables tight The pigment attached to the surface of the seed coat of the pumpkin seeds is completely peeled off, while retaining the integrity of the pumpkin seeds. Compared with the traditional solvent extraction method, it can shorten the extraction time and increase the extraction rate. There are no related patent reports on the application of extraction

Method used

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  • Green pigment of pumpkin seed and extraction method thereof
  • Green pigment of pumpkin seed and extraction method thereof

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Embodiment 1

[0027] Such as figure 1 As shown, the present embodiment provides a pumpkin seed green pigment, and it is extracted by a green extraction method, and the specific method is as follows:

[0028] (1) Clean the pumpkin seeds after shelling, winnowing and machine-selecting, and dry them in microwave with a microwave power of 450W for 10 minutes;

[0029] (2) Mix food-grade absolute ethanol, ethyl acetate and petroleum ether in a volume ratio of 4:3:3 to form a low-toxic organic solvent, which is the extractant. Mix the dried pumpkin seeds and the extractant by 1g: The ratio of 1m L is mixed evenly, and the extraction is assisted by pulse electric field, and the feed liquid is filtered to obtain a primary extraction liquid; the filter residue is mixed with the above-mentioned low-toxicity organic solvent, and the pulse electric field is used to assist extraction again, and the feed liquid is filtered to obtain a secondary extract. Extraction solution; combine two extraction soluti...

Embodiment 2

[0035] Such as figure 1 As shown, the present embodiment provides a pumpkin seed green pigment, and it is extracted by green extraction technology, the specific method is as follows:

[0036] (1) Clean the pumpkin seeds after shelling, winnowing and machine-selecting, and dry them in microwave with a microwave power of 450W for 10 minutes;

[0037] (2) Mix food-grade absolute ethanol, ethyl acetate and petroleum ether in a volume ratio of 6:3:1 to form a low-toxic organic solvent, which is the extractant. Mix the dried pumpkin seeds and the extractant by 1g: The ratio of 1m L is mixed evenly, and the extraction is assisted by pulse electric field, and the feed liquid is filtered to obtain a primary extraction liquid; the filter residue is mixed with the above-mentioned low-toxicity organic solvent, and the pulse electric field is used to assist extraction again, and the feed liquid is filtered to obtain a secondary extract. Extraction solution; two extraction solutions are co...

Embodiment 3

[0042] Such as figure 1 As shown, the present embodiment provides a pumpkin seed green pigment, and it is extracted by green extraction technology, the specific method is as follows:

[0043] (1) Clean the pumpkin seeds after shelling, winnowing, and machine selection, and then dry them in microwave with a microwave power of 430W and a time of 10 minutes;

[0044] (2) Mix food-grade absolute ethanol, ethyl acetate and petroleum ether in a volume ratio of 5:3:2, which is the extractant. Mix the dried pumpkin seeds with the extractant in a ratio of 1g:1mL , using pulsed electric field-assisted extraction, the feed liquid is filtered to obtain a primary extract; the filter residue is mixed evenly with a low-toxicity organic solvent, and pulsed electric field is used to assist extraction again, and the feed liquid is filtered to obtain a secondary extract; combined twice The extraction solution is to obtain the pumpkin seed green pigment extraction solution, wherein the pulse ele...

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Abstract

The invention provides a green pigment of pumpkin seed and an extraction method thereof. The specific extraction method comprises the following steps: carrying out microwave drying on pumpkin seeds after shelling, winnowing and machine selection; adding a low-toxicity solvent while extracting by a pulsed electric field processing unit, and filtering a feed liquid to obtain a primary extract liquid; adding filter residue into the low-toxicity organic solvent, re-extracting, and filtering to obtain a secondary extract liquid; mixing the two extract liquids to obtain a green pigment extract liquid of pumpkin seeds; centrifuging the collected extract liquid, filtering, concentrating, and drying to obtain the powdery green pigment of pumpkin seeds. By using the low-toxicity solvent as the extraction solvent, the extraction method is green and environmentally-friendly and has high extraction rate. The green pigment of pumpkin seeds has pure colour and lustre, high color value and high safety, and can be used as a natural pigment in the food industry. According to the invention, the added value of the pumpkin seed product can be increased, the original natural form of the pumpkin seeds iskept, which is convenient for the next link of processing. The product of the invention has large-scale promotion and application value.

Description

technical field [0001] The invention belongs to the field of natural pigment extraction, in particular to a pumpkin seed green pigment and an extraction method thereof. Background technique [0002] Food coloring can be divided into two categories: natural and artificial. Natural coloring has high safety, soft tones, and some have certain physiological activity, which belongs to functional natural coloring. At present, there are few green pigments in pigment varieties, but the market demand is relatively large. Pumpkin seeds are the mature seeds of Cucurbitaceae pumpkin. Pumpkin seeds are rich in biologically active substances such as essential fatty acids, amino acids, phytosterols, polyphenols, vitamins, and minerals, which can prevent prostate diseases, regulate blood sugar, and regulate cholesterol. In recent years, with the rise of pumpkin seed deep processing industry at home and abroad and the continuous increase in the export of pumpkin seeds, the cultivation area ...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54
Inventor 孙波
Owner NORTHEAST AGRICULTURAL UNIVERSITY