Microbial biocontrol agent for inhibiting bacterial diseases and preparation method thereof
A microbial and bacterial technology, which is applied in the field of microbial biocontrol agents for inhibiting bacterial diseases and its preparation, can solve the problems of weak plant value determination, chemical sterilization, sensitivity to bacteriostatic agents, and inability to share chemical bacteriostatic agents. Achieve the effects of no effect on the activity of the bacterial agent, convenient use, and good comprehensive benefits
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Embodiment 1
[0034] Step 1: Bacillus (named Bacillus DY-3) is subjected to strain rejuvenation and activation treatment in LB medium:
[0035] Step S1: taking the Bacillus strains in a preserved state for use;
[0036] Step S2: After the LB medium is sterilized by a conventional high-temperature sterilizer, pour the LB medium into a 9 cm sterile petri dish on an ultra-clean workbench, about 15 mL per petri dish;
[0037] Step S3: After the LB medium is solidified, use an inoculation loop to pick up the preserved Bacillus species lawn and scratch the board;
[0038] Step S4: place it horizontally for more than half an hour, and wait for the bacterial lawn to be adsorbed on the LB medium;
[0039] Step S5: place the plate upside down in a 37°C incubator and incubate for 30 hours;
[0040] Step S6: Observe the cultured Bacillus strains, select strong colonies, select some of the colonies and inoculate them into new LB medium for culture, repeat 2-3 times, so as to obtain well-growing strain...
Embodiment 2
[0049] Step 1: Bacillus (named Bacillus DY-3) is subjected to strain rejuvenation and activation treatment in LB medium:
[0050] Step S1: taking the Bacillus strains in a preserved state for use;
[0051] Step S2: After the LB medium is sterilized by a conventional high-temperature sterilizer, pour the LB medium into a 9 cm sterile petri dish on an ultra-clean workbench, about 15 mL per petri dish;
[0052] Step S3: After the LB medium is solidified, use an inoculation loop to pick up the preserved Bacillus species lawn and scratch the board;
[0053] Step S4: place it horizontally for more than half an hour, and wait for the bacterial lawn to be adsorbed on the LB medium;
[0054] Step S5: place the plate upside down in a 37°C incubator and incubate for 30 hours;
[0055] Step S6: Observe the cultured Bacillus strains, select strong colonies, select some of the colonies and inoculate them into new LB medium for culture, repeat 2-3 times, so as to obtain well-growing strain...
Embodiment 3
[0064] Step 1: Bacillus (named Bacillus DY-3) is subjected to strain rejuvenation and activation treatment in LB medium:
[0065] Step S1: taking the Bacillus strains in a preserved state for use;
[0066] Step S2: After the LB medium is sterilized by a conventional high-temperature sterilizer, pour the LB medium into a 9 cm sterile petri dish on an ultra-clean workbench, about 15 mL per petri dish;
[0067] Step S3: After the LB medium is solidified, use an inoculation loop to pick up the preserved Bacillus species lawn and scratch the board;
[0068] Step S4: place it horizontally for more than half an hour, and wait for the bacterial lawn to be adsorbed on the LB medium;
[0069] Step S5: place the plate upside down in a 37°C incubator and incubate for 30 hours;
[0070] Step S6: Observe the cultured Bacillus strains, select strong colonies, select some of the colonies and inoculate them into new LB medium for culture, repeat 2-3 times, so as to obtain well-growing strain...
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