High-activity nano-enzymes based on transition metal oxides and derivatives of transition metal oxides as well as acquisition method and application of high-activity nano-enzymes

A transition metal and transition metal ion technology, applied in the cross field, can solve the problems of low catalytic activity of nanozymes, slow nanozyme research and application, and catalytic reaction specificity not as good as natural enzymes, etc. To achieve the effect of flexible control

Active Publication Date: 2019-05-28
NANJING UNIV
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, when studying the catalytic activity of nanozymes of different nanomaterials, it was found that compared with natural enzymes, the catalytic activity of nanozymes is not high, and the specificity of catalytic reactions is not as good as that of natural enzymes.
In addi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-activity nano-enzymes based on transition metal oxides and derivatives of transition metal oxides as well as acquisition method and application of high-activity nano-enzymes
  • High-activity nano-enzymes based on transition metal oxides and derivatives of transition metal oxides as well as acquisition method and application of high-activity nano-enzymes
  • High-activity nano-enzymes based on transition metal oxides and derivatives of transition metal oxides as well as acquisition method and application of high-activity nano-enzymes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 perovskite type transition metal oxide catalytic activity and e g Electron number correlation yields highly active perovskite-type transition metal oxide nanozymes

[0043] 1) ABOs 3-δ Preparation of type perovskite transition metal oxide nanomaterials:

[0044] ABOs 3-δ The preparation methods of type perovskite transition metal oxides and their doped nanomaterials are all sol-gel methods. Specifically, 1.5 mmol of lanthanum nitrate hexahydrate, 1.5 mmol of nickel nitrate hexahydrate (chromium nitrate nonahydrate or iron nitrate nonahydrate), and 12 mmol of citric acid were uniformly dispersed in 200 mL of water. After the solution was fully stirred and mixed, 1.5mL of ethylene glycol was added, and the stirring was continued. The solution was heated to 90°C until the solution became a viscous gel-like liquid. Put the gel into an oven and heat at 180°C for 5 hours to obtain a solid perovskite precursor. Put the perovskite precursor into a calciner at...

Embodiment 2

[0049] Embodiment 2 flexibly adjusts e by doping g The number of electrons and its nanozyme activity obtained highly active La 0.5 Sr 0.5 FeO 3-δ , SrFeO 3-δ

[0050] 1) LaFeO 3-δ , La 0.5 Sr 0.5 FeO 3-δ , SrFeO 3-δ The preparation is also synthesized with reference to the method in Example 1).

[0051] 2) Prepare substrate solution for measuring peroxidase mimic activity: sodium acetate buffer (pH 4-5, 810 μL), 500 mM H 2 o 2 (100 μL), 10 mM TMB (80 μL).

[0052] 3) Preparation of nanozyme solution: the concentration of all transition metal oxide nanozymes was 1 mg / mL, and they were uniformly dispersed in water by ultrasound.

[0053] 4) Determination of peroxidase mimetic activity: Take 10 μL of the above-mentioned nanozyme solution and add it to 990 μL of the substrate solution. The reaction temperature is 40°C. Reaction rates were calculated from the change in absorbance over 60 seconds.

[0054] Depend on figure 2 It can be seen that due to the LaFeO 3-δ ...

Embodiment 3 2

[0055] The acquisition of embodiment 3 binary metal oxide nanozymes

[0056] 1) Preparation of binary metal oxide nanomaterials:

[0057] MnO 2 : About 2g KMnO 4 with about 0.8g MnSO 4 ·H 2 O was added to about 25mL of water, mixed and stirred evenly, then placed in a high-pressure reactor at 150-180°C for 10-15 hours, centrifuged to obtain the product, washed with water several times, and then dried.

[0058] mn 2 o 3 : About 0.5g KMnO 4 Mix with about 0.6g of glucose in about 10mL of water, stir evenly and put it into a high-pressure reactor at 150-180°C for 8-12 hours. The obtained product is centrifuged, washed and dried, and finally calcined at 500-600°C for 4-6 hours to obtain the final product .

[0059] CoO: About 0.1g CoSO 4 ·7H 2 O and about 0.3g urea were dissolved in the mixture of ethanol and water, stirred evenly, added ammonia water drop by drop, after stirring for 30 minutes, placed in a high-pressure reactor at 120-150°C for 10-12 hours, and the obta...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses high-activity nano-enzymes based on transition metal oxides and derivatives of the transition metal oxides as well as an acquisition method and application of the high-activitynano-enzymes. The acquisition method comprises the synthesis of the nano-enzymes, measurement of activities and acquisition of the corresponding relation between the activities of the transition metal oxide nano-enzymes and the electronic structures of transition metal ions in the transition metal oxides, wherein when the eg electron number of the transition metal ions is 1 or about 1, the transition metal oxide nano-enzymes are the high-activity transition metal oxide nano-enzymes; or when the eg electron number of the transition metal ions is not 1 or about 1, metal ions are doped or otherways are taken to regulate the valence states of the central ions so as to enable the eg electron number to be 1 or about 1 to obtain the high-activity transition metal oxide nano-enzymes. According to the invention, the activities of the nano-enzymes based on the transition metal oxides are quantitatively measured, and shows very good correlation with the eg electron number of the transition metal oxides, so that flexible regulation and control of the activities of various nano-enzymes is realized, and the high-activity nano-enzymes based on the transition metal oxides and the derivatives ofthe transition metal oxides are obtained.

Description

technical field [0001] The invention belongs to the interdisciplinary fields of functional materials and biomimetic catalysis, and specifically relates to a transition metal oxide-based high-activity nanozyme and its obtaining method and application, and can be applied to the design of a transition metal oxide-based high-activity nanozyme. The synthesis of oxide biocatalytic materials has broad application prospects and guiding significance. Background technique [0002] Enzymes are a type of macromolecular protein with catalytic activity in organisms. The metabolism and most activities of organisms are inseparable from the catalysis of enzymes. It is with the participation of enzymes that organisms can efficiently and accurately complete life activities. However, the content of natural enzymes in organisms is scarce, and there are problems such as difficult extraction and harsh storage and transportation conditions. In the process of application in actual production activit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): B01J23/83B01J23/26B01J23/78B01J23/889G01N21/31
Inventor 魏辉王小宇秦溧
Owner NANJING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap