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Parasite staining fluid

A dyeing solution and parasite technology, applied in the preparation of test samples, etc., can solve the problems of physical injury, incomplete shape, and lower detection rate of workers, and achieve simple and fast methods, strong dyeing power, and clear coloring Effect

Active Publication Date: 2019-05-28
HUBEI TAIKANG MEDICAL EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The birth of histopathology has a history of more than 150 years. Eosin solution is one of the commonly used pathological reagents. It is mainly used to stain tissue sections. Most of the current eosin staining solutions contain formaldehyde, xylene, mercuric oxide, Ammonia, etc., are toxic chemical reagents, not only causing harm to the health of the staff, but also polluting the environment
[0003] Single coloring for parasites, unclear coloring, incomplete shape, and easy to identify a single type of parasite, while other types of parasites are not easy to identify, reducing the detection rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1) Add 5% by weight of sodium chloride to water at a temperature of 30°C, stir evenly, then heat up to 51°C, add 5% by weight of eosin, 2% by weight of azure, 3 % by weight of methylene blue, 0.5% by weight of tocopherol, 0.2% by weight of nano-pearl powder, and 0.6% by weight of ethyl acetate, stirred evenly, and then ultrasonicated for 5 minutes at a water bath temperature of 32°C to obtain the first solution;

[0044] 2) Add 0.01% by weight of neutral glycerin to 10% by weight of methanol, sonicate for 2 minutes at a water bath temperature of 35°C, then add 1% by weight of Tween-20, and sonicate for 1min at a water bath temperature of 40°C , to obtain the second solution;

[0045] 3) The first solution in step 1) and the second solution in step 2) were mixed into a vessel, and the vessel was sonicated in a water bath at a water bath temperature of 33° C. for 1 min to obtain a parasite staining solution.

[0046] Water is distilled water.

Embodiment 2

[0048] 1) Add 6% by weight of sodium chloride to water at a temperature of 39°C, stir evenly, heat up to 55°C, add 8% by weight of eosin, 5% by weight of azure, 6% by weight to the heated water % by weight of methylene blue, 1% by weight of tocopherol, 0.8% by weight of nano-pearl powder, and 0.9% by weight of ethyl acetate, stirred evenly, and then ultrasonicated for 8 minutes at a water bath temperature of 35°C to obtain the first solution;

[0049] 2) Add 0.02% by weight of neutral glycerin to 50% by weight of methanol, sonicate for 3 minutes at a water bath temperature of 35°C, then add 2% by weight of Tween-20, and sonicate for 2 minutes at a water bath temperature of 40°C , to obtain the second solution;

[0050] 3) The first solution in step 1) and the second solution in step 2) were mixed into a vessel, and the vessel was sonicated in a water bath at a water bath temperature of 33° C. for 3 minutes to obtain a parasite staining solution.

[0051] Water is deionized w...

Embodiment 3

[0053] 1) 5.6% by weight of sodium chloride is added to water at a temperature of 35°C, after stirring evenly, the temperature is raised to 54°C, and 5% by weight of eosin, 3% by weight of azure, 4% by weight are added to the heated water. % by weight of methylene blue, 0.6% by weight of tocopherol, 0.5% by weight of nano-pearl powder, and 0.8% by weight of ethyl acetate, stirred evenly, and then ultrasonicated for 7 minutes at a water bath temperature of 33°C to obtain the first solution;

[0054] 2) Add 0.01% by weight of neutral glycerin to 200% by weight of methanol, sonicate for 3 minutes at a water bath temperature of 35°C, then add 1% by weight of Tween-20, and sonicate for 2 minutes at a water bath temperature of 40°C , to obtain the second solution;

[0055]3) The first solution in step 1) and the second solution in step 2) were mixed into a vessel, and the vessel was sonicated in a water bath at a water bath temperature of 33° C. for 1-3 minutes to obtain a parasite...

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Abstract

The invention discloses parasite staining fluid. The parasite staining fluid is prepared from, by weight, 5%-8% of eosin, 2%-5% of celeste, 3%-6% of methylene blue, 10%-50% of methyl alcohol, 0.01%-0.02% of neutral glycerine, 5%-6% of sodium chloride, 1%-2% of twain-20, 0.5%-1% of tocopherol, 0.2%-0.8% of nanometer pearl powder, 0.6%-0.9% of ethyl acetate and the balance water. The parasite staining fluid is suitable for staining of parasites, various microorganisms, blood cell smears, bone marrow smears, exfoliative cell smears, chromosome banding, cell apoptosis detection and the like, onlyone staining fluid is used, fixation is not required, fixation and staining are integrated, and multiple inspection items of the parasites, germs, nuclear heterogeneous cells, cell classifying counting and the like can be simultaneously conducted on a slice; a method is simple and quick, staining power is high, coloring is clear, the form is complete, distinguishing is easy, and the detection rateis high.

Description

technical field [0001] The invention relates to the technical field of sanitation preparations, in particular to a parasite dyeing solution. Background technique [0002] The birth of histopathology has a history of more than 150 years. Eosin solution is one of the commonly used pathological reagents. It is mainly used to stain tissue sections. Most of the current eosin staining solutions contain formaldehyde, xylene, mercuric oxide, Ammonia, etc., are toxic chemical reagents, which not only cause harm to the health of the staff, but also pollute the environment. [0003] The coloring of parasites is single, the coloring is not clear, the shape is incomplete, and it is easy to identify a single type of parasite, while other types of parasites are not easy to identify, which reduces the detection rate. Contents of the invention [0004] The invention provides a parasite staining solution. [0005] The scheme of the present invention is: [0006] A parasite staining solut...

Claims

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Application Information

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IPC IPC(8): G01N1/30
Inventor 肖国林
Owner HUBEI TAIKANG MEDICAL EQUIP
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