A kind of cyclic (isoleuc-isoleuence) dipeptide, its preparation method and application
A technology of seed and action, applied in botany equipment and methods, cyclic peptides, applications, etc., can solve the problems that hinder the healthy development of modern agricultural industry, reduce the quality of agricultural products, soil compaction and acidification, etc., and achieve easy large-scale batch production, The effect of short production cycle and high activity
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Embodiment 1
[0049] Embodiment 1: the fermentation process optimization of Paecilomyces variotii (Paecilomyces variotii)
[0050] 1) Optimization of fermentation medium
[0051] The Paecilomyces variotii activated on the PDA medium was inoculated into the seed medium for cultivation, and the fermentation was carried out according to the traditional fermentation process. After the cultivation, the plate colonies were counted to calculate the biomass of the bacteria. The formula of the traditional fermentation process fermentation medium is as shown in Table 1 below:
[0052] Table 1 is the traditional fermentation process fermentation conditions
[0053]
[0054]
[0055] Substitute corn flour, soluble starch, glucose, and lactose for sucrose in the traditional fermentation medium, keep the other ingredients in the fermentation medium unchanged, the inoculum size is 2%, the temperature is 28°C, the initial pH is 7.0, and the stirring speed is 180r / min. After culturing for 7 days, th...
Embodiment 2
[0066] Example 2: Fermentation of Paecilomyces variotii (Paecilomyces variotii) and preparation of cyclic dipeptide compounds
[0067] 1) Inoculate Paecilomyces variotii in PDA solid medium, and culture at a constant temperature of 25°C for 5 days; use a puncher agar to dig out the activated bacteria and inoculate them into 200ml seed medium (PDA liquid medium) The triangular flask was shaken and cultured at 25°C and 180r / min for 5-7 days on a shaking table as the first-level seed solution; the first-level seed solution was placed in a 5L seed tank, and the second-level seed tank was fermented; the determined most Put the suitable culture solution in a fermenter for sterilization at 121°C for 20 minutes, inoculate the first-grade seeds after cooling, the inoculum amount is 6%, the temperature is 25.8°C, the initial pH is 7.2, the stirring speed is 160r / min, cultivate for 5 days, and take samples for testing every day. obtain the target product. The formula of the fermentation...
Embodiment 3
[0070] Example 3: Effects of Cyclic Dipeptide Compounds on Wheat Germination under Salt Stress
[0071] (1) Soak wheat seeds (wheat variety: Yannong 15) in water and different concentrations of cyclic dipeptide solutions for 2 hours (the concentrations of soaking cyclic dipeptides are 20, 30, and 40 ng / ml respectively): ① Common germination conditions: take soaked seeds separately Put 50 good seeds in a large petri dish with filter paper, add clear water to a height of 50% of the seed height, and pay attention to replenishing water during germination. Germinate under the condition of 15-25°C in the dark and variable temperature, and count the longest length and number of fibrous roots; ②Simulate salt-resistant conditions: replace the clear water added to the medium in the above test with 0.6% NaCl saline, 0.8% NaCl Salt water, while using clear water as a control, and other conditions unchanged, the longest length and number of fibrous roots were counted.
[0072] Calculation...
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