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Method for preparing tobacco essence spice with glossy ganoderma or antrodia camphorata as fermentation raw material

A technology for flavoring and fermenting raw materials for tobacco, which is applied in the fields of essential oils/fragrances, preparation of tobacco, tobacco, etc. It can solve the problems of lack of aroma quality, aroma intensity, and fragrance retention time, so as to protect the liver, enrich the source of raw materials, and relieve damage Effect

Inactive Publication Date: 2019-05-31
CHINA TOBACCO SICHUAN IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the existing research on the preparation of flavors and fragrances from Ganoderma lucidum and Antrodia camphorata as raw materials by fermentation is still at a relatively primitive stage, and most people simply use Ganoderma lucidum and Antrodia camphorata After cultivating Antrodia camphorata, flavors and fragrances are extracted from the obtained culture fluid. There is a lack of research on improving the quality of aroma, aroma intensity, and fragrance retention time of these flavors and fragrances. This is undoubtedly the same as the tobacco industry trying to use these flavors Flavors Contradict Ideas That Increase Market Competitiveness of Tobacco Products

Method used

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  • Method for preparing tobacco essence spice with glossy ganoderma or antrodia camphorata as fermentation raw material
  • Method for preparing tobacco essence spice with glossy ganoderma or antrodia camphorata as fermentation raw material
  • Method for preparing tobacco essence spice with glossy ganoderma or antrodia camphorata as fermentation raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: Ganoderma lucidum + Sophora japonica

[0058] Specific steps are as follows:

[0059] (1) Take 80mL seed culture medium and place it in a 250mL shake flask, take a piece of 1cm 2 Ganoderma lucidum stored at 4°C was inoculated into the seed medium, and the -1 Cultivate under the condition of 7d, obtain seed solution;

[0060] (2) Take 100g of fermentation medium (sterilized at 105°C for 20min) and place it in a 250mL shake flask, inoculate the seed liquid into the fermentation medium according to the parameters in Table 2 for fermentation, and obtain fermentation product A 0 、A 1 、A 2 、A 3 ; where, A 0 group is the blank control group.

[0061] With 2-octanol as the internal standard (final concentration 8.2ppb), the fermentation product A 0 、A 1 、A 2 、A 3 Analyze according to the analysis method recorded in the flavor and fragrance solid-phase microextraction-gas analysis method, wherein, the fermented product A 0 、A 1 The analysis results are...

Embodiment 2

[0078] Embodiment 2: Antrodia camphorata+chrysanthemum

[0079] Specific steps are as follows:

[0080] (1) Take 80mL seed culture medium and place it in a 250mL shake flask, take a piece of 1cm 2 The Antrodia camphorata stored at 4°C was inoculated into the seed medium, and at a temperature of 28°C and a rotation speed of 110r min -1 Cultivate for 10 days under the condition of , and obtain the seed solution;

[0081] (2) Take 100g of fermentation medium (sterilized at 105°C for 20min) and place it in a 250mL shake flask, inoculate the seed liquid into the fermentation medium according to the parameters in Table 6 for fermentation, and obtain fermentation product B 0 , B 1 , B 2 , B 3 ; where, B 0 group is the blank control group.

[0082] ferment B 0 , B 1 , B 2 , B 3 Analyze according to the analysis method recorded in the flavor and fragrance solid phase microextraction-gas analysis method, wherein, the fermented product B0 , B 1 The analysis results are shown ...

Embodiment 3

[0137] Embodiment 3: the preparation of tobacco

[0138] Specific steps are as follows:

[0139] 1. Extraction

[0140] With the fermented product A that embodiment 1 obtains 1 And the fermented product B that embodiment 2 obtains 1 Extract according to the parameter conditions of scheme one, scheme two, scheme three, scheme four respectively, obtain flavors and fragrances for tobacco A, B, C, D, E, F, G, H; Wherein, the extraction conditions of each scheme are as follows:

[0141] Option 1: Add 30% ethanol (v / v) to the fermented product at a volume ratio of 1:3, extract at 150 rpm for 2 hours, and centrifuge the extract at 10,000 rpm for 10 minutes to obtain the supernatant, which is the flavor and fragrance for tobacco;

[0142] Scheme 2: Add 50% ethanol (v / v) to the fermented product at a volume ratio of 1:4, extract at 200 rpm for 2 hours, and centrifuge the extract at 10,000 rpm for 10 minutes to obtain the supernatant, which is the flavor and fragrance for tobacco;

...

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Abstract

The invention discloses a method for preparing a tobacco essence spice with glossy ganoderma or antrodia camphorata as a fermentation raw material and belongs to the technical field of microorganism fermentation. According to the preparation method, the glossy ganoderma or the antrodia camphorata is inoculated into a fermentation culture medium with flowers for fermentation, the fragrance characteristics, such as fragrance quality and fragrance intensity, of the tobacco essence spice obtained through fermentation of the glossy ganoderma and the antrodia camphorata are greatly improved, the fragrance time of the tobacco essence spice obtained through fermentation of the glossy ganoderma and the antrodia camphorata is greatly prolonged, and accordingly the tobacco essence spice with improvedfragrance is obtained. According to the method, the technology is simple, the raw material source is rich, the cost is low, and the method can be applied to industrial production; the tobacco essencespice prepared through the method is pleasant in fragrance, further contains biological activity substances, such as active polysaccharide, active protein, flavonoid compounds and triterpenoid, produced through fungi for food and medicine, has certain activities such as immune adjustment, tumor resistance, oxidation resistance, blood sugar reduction and liver protection, and can be widely appliedto the field of tobacco.

Description

technical field [0001] The invention relates to a method for preparing tobacco essence and fragrance by using Ganoderma lucidum or Antrodia camphorata as fermentation raw materials, and belongs to the technical field of microbial fermentation. Background technique [0002] The fragrance and fragrance industry is an industry with high technological content, strong matching and high correlation with other industries in the national economy. As a kind of fragrance product, it can bring people a sense of comfort and joy, and to a certain extent relieve the pressure of contemporary society. The tension brought by the fast-paced lifestyle, therefore, it is widely used in the flavoring of food, medicine, cosmetics and tobacco products, occupying an unshakable position. [0003] Especially in the tobacco industry, as the controversy between smoking and health issues becomes increasingly prominent, an important challenge facing the tobacco industry is to reduce the tar content of tob...

Claims

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Application Information

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IPC IPC(8): C11B9/02A24B3/12A24B15/26
Inventor 罗诚李东亮张倩颖耿宗泽张迪丁重阳徐萌萌赵丽婷刘元法
Owner CHINA TOBACCO SICHUAN IND CO LTD
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