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Enzyme-linked immunosorbent assay device and method used for on-site diagnosis

An enzyme-linked immunosorbent adsorption and detection device technology, which is applied in the field of immunoassays, can solve the problems of not being able to give quantitative results and limited application occasions, and achieve the effects of shortening the detection time, convenient storage and transportation, and convenient operation

Active Publication Date: 2019-06-04
WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Test strips (such as pregnancy test strips) are a type of POCT tool that can detect biomolecules without any equipment, but such tools can only perform qualitative detection of biomarkers, and cannot give quantitative results, so the applicable occasions are very limited

Method used

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  • Enzyme-linked immunosorbent assay device and method used for on-site diagnosis
  • Enzyme-linked immunosorbent assay device and method used for on-site diagnosis
  • Enzyme-linked immunosorbent assay device and method used for on-site diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Such as figure 1 and 2 As shown, an enzyme-linked immunosorbent assay device for on-site diagnosis includes a bottom plate 2, a cover plate 1 and a sealing layer 3. The bottom plate 2 is connected to the cover plate through a connecting piece 4, and the two can be relatively closed or opened. The base plate 2 is provided with a plurality of first reaction holes 6 along its transverse direction, and a scale line 5 is provided along the longitudinal direction of the base plate; the cover plate 1 is provided with a plurality of second reaction holes 8, and when the cover plate and the base plate are bonded together, the first reaction holes 6 overlaps with the second reaction hole 8, the second reaction hole 8 communicates with the indicator hole 9 through the gas channel 10, and the indicator hole 9 communicates with the liquid outlet 7 through the reading channel 11; the gas channel 10 is shorter than the reading channel 11 The sealing layer 3 is located between the bas...

Embodiment 2

[0030] Taking the detection of carcinoembryonic antigen (CEA) as an example to illustrate the specific manufacturing method of the device:

[0031] 1. Structural production: first use the drawing software such as AUTOCAD to design the structure of the cover plate and the bottom plate, and then read the designed file through the control software of the laser cutting machine, and cut out two rectangular PMMA plates of equal size (size 76mm×25mm×1mm), one is used as a cover plate, and one is used as a bottom plate, and then the structures on the two substrates are etched by laser to form figure 2 The structure shown (the diameter of the first and second reaction holes is 5mm, and the depth is 0.5mm; the width and length of the gas channel are respectively 1mm and 10mm; the width and length of the reading channel are respectively 1mm and 50mm; the liquid outlet The diameter is 5mm); then the double-sided tape is cut into a rectangle with the same size as the cover plate and the s...

example 3

[0044] The processing and operation method for another example of carcinoembryonic antigen (CEA) detection is given below, and the device is processed based on photolithography technology, specifically:

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Abstract

The invention discloses an enzyme-linked immunosorbent assay device and method used for on-site diagnosis. The device comprises a base plate and a cover plate. A first reaction hole is arranged alonga horizontal direction of the base plate. The surface of the first reaction hole is combined with a capture antibody and a detection antibody labeled with catalase or platinum nanoparticles. A scale line is arranged along a vertical direction of the base plate. The cover plate is provided with a second reaction hole. The second reaction hole is connected to an indicator hole through a gas channel.The indicator hole is connected to a liquid outlet through a reading channel. The second reaction hole stores urea peroxide and sodium citrate. The indicator hole contains indicator powder. The ureaperoxide is reacted with the sodium citrate to form hydrogen peroxide, and the hydrogen peroxide reacts with the catalase or the platinum nanoparticles labeled on the detection antibody to generate oxygen. An indicator solution is pushed by the oxygen to enter into the reading channel to form an indicator strip, a length of the indicator strip can reflect a concentration of a sample to be tested,and a result is visible to naked eyes without any device-assisted reading.

Description

technical field [0001] The invention belongs to the technical field of immunoassay, and in particular relates to an enzyme-linked immunosorbent detection device and method for on-site diagnosis (Point-of-care testing, POCT), which can quantitatively detect various biomarkers based on the enzyme-linked immune amplification reaction Concentration, the result is visible to the naked eye, without any equipment to assist the reading, and can be widely used in the determination of the concentration of biochemical indicators such as biological macromolecules or chemical small molecules in various samples. Background technique [0002] Enzyme-linked immunosorbent assay (ELISA) is a method commonly used in the detection of biomarkers. The principle of traditional ELISA is to adsorb known antigens or antibodies on the surface of solid phase carriers, and then the specific recognition effect based on antigens and antibodies will have a catalytic effect. The introduction of effective en...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/543
Inventor 李颖杨运煌胡锐
Owner WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI