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Simplified fungus 1-3-beta-D glucan detection kit

A detection kit, dextran technology, which is applied in the direction of material analysis, biochemical instruments, and biochemical cleaning devices by observing the impact on chemical indicators, which can solve the problems of easy introduction of interfering substances, long detection time, and low accuracy. and other problems, to achieve the effect of shortening the operation time, reducing the procurement cost and facilitating the operation.

Pending Publication Date: 2019-06-11
DYNAMIKER BIOTECH TIANJIN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] CN 102305787 A patent on a fungal 1-3-β-D glucan detection kit, the kit described in the patent has disadvantages such as cumbersome operation, low accuracy, easy introduction of interfering substances during operation, and long detection time

Method used

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  • Simplified fungus 1-3-beta-D glucan detection kit
  • Simplified fungus 1-3-beta-D glucan detection kit
  • Simplified fungus 1-3-beta-D glucan detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Preparation of detection kit

[0027] Such as figure 1 As shown, the kit includes a box body 10, an integrated reagent filling and reaction device 20, a main reaction agent 30, a main agent compound solution 40, a sample processing solution 50, and a quality control product 60:

[0028] Preparation of main reaction agent 30: Add factor B, factor C inhibitor—serine protease inhibitor, and peptide color matrix Boc-Leu-Gly-Arg—PNA-Cl or Boc-Ile-Gly-Arg— to the lysate of horseshoe crab blood cells PNA-Cl, so that the content of serine protease inhibitor in each milliliter of solution is 0.5-3mg, and the content of peptide color matrix is ​​0.5-2mg. Stir and mix the solution evenly and dispense it into the reaction cup, the volume is between 50-200ul , Vacuum freeze-drying is carried out to obtain the main reaction agent 30. The excipient is one or a combination of mannitol, lactose, glycine, and skim milk.

[0029] Preparation of main compound solution 40: Weigh Tris ...

Embodiment 2

[0033] Example 2 Use of integrated reagent filling and reaction device

[0034] Such as figure 2 As shown, a reagent filling and reaction device provided by the present invention includes a reaction cup 1, a rear chamber 2, a front chamber 3, and a push rod 4 connected in sequence, and the push rod 4 is inserted into the front chamber 3 and oriented relative to the front chamber 3. The inner movement pierces the sealing film 33 at the bottom of the front cabin 3, so that the push rod 4 and the front cabin 3 form an integral body. The rear cabin 2 moves inward to pierce the cone structure at the bottom of the rear cabin 2, the outer wall of the front cabin 3 and the inner wall of the rear cabin 2. In a sealed fit, the push rod 4, the front chamber 3, and the rear chamber 2 form an integrally moving inner cavity of the reaction cup 1, and the outer wall of the rear chamber 2 and the inner wall of the reaction cup 1 are in a sealed fit.

[0035] The outer sides of the upper end of th...

Embodiment 3

[0044] Example 3 Detection steps

[0045] Specific use:

[0046] 1) Sample processing: Aseptically, draw 4ml of venous blood with a dedicated pyrogen-free vacuum serum collection tube, shake it gently, leave it at room temperature for about 30 minutes, and then centrifuge it in a low-temperature centrifuge at 1000 rpm for 10-15 minutes, use a pipette or 15-80ul of serum is sucked by a Pasteur pipette and added to the front chamber 3 containing the sample processing solution 50, placed in the automatic microplate reader (model), shaken and mixed, and incubated for 5-15 minutes.

[0047] 2) After the incubation is completed, the automatic microplate reader will automatically press the integrated reagent filling and reaction pack 20, so that the push rod 4 pierces the sealing film at the lower end of the front chamber 3, and the sample processing solution 50 in the front chamber 3 flows with the sample together Rear chamber 2, mix with the main agent compound solution 40 in rear chambe...

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Abstract

The invention relates to the technical field of detection of 1-3-beta-D glucan, and particularly discloses a simplified fungus 1-3-beta-D glucan detection kit. The kit comprises a kit body, an integrated reagent filling and reaction device, a reaction main agent, a main agent reconstitution fluid, a sample treating fluid and a quality control product; the integrated reagent filling and reaction device is composed of a reaction cup, a rear bin, a front bin and a push rod; and an integrated detection device can be formed by adding the reaction main agent, the main agent reconstitution solution and the sample treating fluid into the reaction cup, the rear bin and the front bin separately. The experiment operation is convenient, experiment tools are reduced, professionals are not needed, experiments are simple and rapid, and the detection accuracy is higher.

Description

Technical field [0001] The invention relates to the field of fungal detection, in particular to a simplified fungal 1-3-β-D glucan detection kit. Background technique [0002] At present, a diagnostic method for invasive fungal infections that has attracted much attention is to detect the fungal cell wall component 1-3-β-D glucan. Studies have shown that, except for conjugate bacteria and cryptococcus, the cell walls of all fungi contain 1-3-β-D glucan, which accounts for more than 50% of the fungal cell wall components. It is a unique component of the fungal cell wall, especially in yeast-like fungi. Its content is higher; bacteria, viruses, other pathogenic bacteria and human cells do not have this ingredient. Once pathogenic bacteria invade deep tissues and organs of the human body or blood, the body can be processed by phagocytic cells, digestive enzymes, etc. to release 1-3-β-D glucan from its cell wall into the blood or other body fluids to increase its content . Because...

Claims

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Application Information

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IPC IPC(8): C12M1/34G01N21/78
Inventor 王保学薛知信周泽奇粟艳
Owner DYNAMIKER BIOTECH TIANJIN
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