Method for enriching and screening a target substance, such as cells, from sample

Abstract

The invention relates to a method for enriching and screening a target substance, such as cells, from a sample, wherein the device comprises a plurality of porous net-shaped substrates which are arranged in parallel, the porous net-shaped base body comprises a net-shaped base body formed by a plurality of mutually-crossed net wires, a plurality of screen holes formed by a plurality of net lines which are arranged in a crossed mode, a capture layer formed on the reticulate substrate, wherein the capture layer comprises a capture object which can be specifically combined with the target object,and the screen holes of the adjacent two-layer porous net-shaped base bodies are arranged in a staggered mode. According to the invention, the capture rate of the target object is improved under the condition that the high flux is ensured.

Description

technical field [0001] The present invention relates to a device for enrichment of screening targets such as cells, bacteria or biomolecules from a sample. Background technique [0002] In the detection of blood and biological samples, rare cells and rare molecules in the test samples are often detected. The successful separation and enrichment of these rare targets or molecules is the key to the accuracy of the detection. For example, in human production and life, it is necessary to detect human body fluids, food, and detection objects in the natural environment. For example, in medical practice, food safety, and environmental monitoring, it is often necessary to detect cells (such as circulating tumor cells, nucleated red blood cells); Microorganisms (such as Streptococcus pneumoniae, Legionella, fungi and special pathogens such as mycoplasma, chlamydia, rickettsia, spirochetes, viruses, etc. in medical testing human fluids; microorganisms such as coliform bacteria, pathog...

AI Technical Summary

Problems solved by technology

However, since the porous network matrix in this patent is only one layer or multi-layer stacked, and when multiple layers are stacked, there is no angle between the multi-layer porous network matrix, that is, the multi-layer porous network The sieve holes of the matrix are overlapped, so when passing through the first layer of multi-channel network matrix, circulating tumor cells are not captured, because the contact area between the cells and the rest of the multi-channel network matrix passing through is relatively small. Small, the uncaptured circulating tumor cells will pass directly through the rest of the porous network matrix without being captured, resulting in a low capture rate of the patented device

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