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Preparation method of silk fibroin microsphere drug sustained-release carrier and application thereof

A technology of silk fibroin microspheres and slow-release carriers, which is applied in the fields of botanical equipment and methods, biochemical equipment and methods, and applications, can solve the problems of inability to take into account the fast initial release speed and slow release of drugs, and achieve excellent Slow release effect, good application prospect, excellent biocompatibility effect

Pending Publication Date: 2019-06-14
李琳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problem that the microsphere slow-release carrier in the prior art cannot take into account the fast initial release speed and slow release of the drug, and further provide a preparation method and application of the silk fibroin microsphere drug slow-release carrier

Method used

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  • Preparation method of silk fibroin microsphere drug sustained-release carrier and application thereof
  • Preparation method of silk fibroin microsphere drug sustained-release carrier and application thereof
  • Preparation method of silk fibroin microsphere drug sustained-release carrier and application thereof

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Embodiment 1

[0035] This embodiment provides a preparation method of a silk fibroin microsphere drug slow-release carrier, comprising the following steps:

[0036] 1) Dissolve the recombinant spidroin fiber with deionized water and ethanol, after dissolving, filter through gauze and place it in a dialysis bag with a molecular weight cut-off of 8-10kD for dialysis for 4 days, filter and concentrate to obtain a silk fibroin solution with a concentration of 0.8wt% ;

[0037]2) The silk fibroin solution obtained in step 1) is placed in a dialysis bag (the molecular weight cut-off of the dialysis bag is 8-10kD), and nanomaterials are added into the dialysis bag (the nanomaterials are nano titanium dioxide particles and nano sheep bone particles, The mass ratio of the nano-material to the silk fibroin solution is 0.1:100, the mass ratio of the nano-titanium dioxide particles to the nano-sheep bone particles is 0.3:1, and the particle diameter of the nano-titanium dioxide particles is 30-50nm, T...

Embodiment 2

[0041] This embodiment provides a preparation method of a silk fibroin microsphere drug slow-release carrier, comprising the following steps:

[0042] 1) Dissolve the recombinant spidroin fiber with deionized water and ethanol, after dissolving, filter through gauze and place it in a dialysis bag with a molecular weight cut-off of 8-10kD for dialysis for 4 days, filter and concentrate to obtain a silk fibroin solution with a concentration of 1.0wt% ;

[0043] 2) The silk fibroin solution obtained in step 1) is placed in a dialysis bag (the molecular weight cut-off of the dialysis bag is 8-10kD), and nanomaterials are added into the dialysis bag (the nanomaterials are nano titanium dioxide particles and nano sheep bone particles, The mass ratio of the nano-material to the silk fibroin solution is 0.3:100, the mass ratio of the nano-titanium dioxide particles to the nano-sheep bone particles is 0.5:1, and the particle diameter of the nano-titanium dioxide particles is 30-50nm, ...

Embodiment 3

[0047] This embodiment provides a preparation method of a silk fibroin microsphere drug slow-release carrier, comprising the following steps:

[0048] 1) Dissolve the recombinant spidroin fiber with deionized water and ethanol, after dissolving, filter through gauze and place it in a dialysis bag with a molecular weight cut-off of 8-10kD for dialysis for 3 days, filter and concentrate to obtain a silk fibroin solution with a concentration of 0.9wt% ;

[0049] 2) The silk fibroin solution obtained in step 1) is placed in a dialysis bag (the molecular weight cut-off of the dialysis bag is 8-10kD), and nanomaterials are added into the dialysis bag (the nanomaterials are nano titanium dioxide particles and nano sheep bone particles, The mass ratio of the nano-material to the silk fibroin solution is 0.2:100, the mass ratio of the nano-titanium dioxide particles to the nano-sheep bone particles is 0.4:1, and the particle diameter of the nano-titanium dioxide particles is 30-50nm, ...

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Abstract

The invention relates to the field of preparation of a drug sustained-release carrier, in particular to a preparation method of a silk fibroin microsphere drug sustained-release carrier and an application thereof. The method for preparing the silk fibroin microsphere drug sustained-release carrier provided by the invention comprises the following steps: dissolving the recombinant spider silk protein fiber, and performing steps of filtering, dialysis, and concentrating to obtain a silk fibroin solution; placing the silk fibroin solution in a dialysis bag, adding a nano material to the dialysisbag, and then placing the dialysis bag in a water bath at 22 DEG C to 25 DEG C, and processing the materials in a uniform shaker to obtain a microsphere suspension; washing the microsphere suspensionwith deionized water, and performing steps of centrifugation and freeze drying to obtain thesilk fibroin microsphere drug sustained-release carrier; wherein the nano material is nano-titanium dioxideparticles and nano-goat bone particles. The drug sustained-release carrier obtained by the preparation method provided by the invention can combine the faster initial release rate of the drug and theexcellent sustained-release effect, and has the good application prospect.

Description

technical field [0001] The invention relates to the field of preparation of drug slow-release carriers, in particular to a preparation method and application of silk fibroin microsphere drug slow-release carriers. Background technique [0002] In daily life, because some drugs are irritating to the esophagus and gastric mucosa, easy to volatilize or easily inhaled into the trachea, etc., they are often packed into capsules to avoid the influence of water, air and light, which not only prevents the drug properties from being destroyed, but also prevents the drug from being damaged. Protects the digestive organs and respiratory tract. In addition, the capsule can delay the release of the drug and position the release of the drug, so as to achieve the functions of slow release and prolonged effect and positioning intelligent drug delivery. At present, the commonly used carrier materials for microsphere drug delivery systems include biodegradable materials such as sodium algina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/02A61K47/42A61K47/46A61K9/16C12N15/12C12N15/70C07K14/435
Inventor 李琳李春车锐
Owner 李琳
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