Rice rose fermentation primary pulp and preparation method and application thereof
A technology for fermenting puree and rice, which is applied in pharmaceutical formulations, cosmetic preparations, dressing preparations, etc. It can solve the problems of single ingredients and high efficiency, and achieve mass production, save production costs, and simplify production steps.
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Embodiment 1
[0046] A kind of preparation method of rice rose fermented protoplasm, concrete steps are as follows:
[0047] 1. Activation of strains
[0048] Pick a ring of Pichia fabianii colony in the liquid medium, put it in a shaker to activate the strain, and obtain the activated Pichia fabianii liquid (OD value between 0.8 and 1.2) ;
[0049] 2. Purification of strains
[0050] The activated bacterial solution obtained in step 1 was serially diluted and plated so as to obtain a single colony to obtain purified Pichia fabiani;
[0051] 3. Expansion and cultivation of strains
[0052] Inoculate the purified bacterial classification obtained in step 2 into the wort medium (pH value is 7.0), cultivate in a shaker at 30° C., and when the OD value=1.0, obtain the fermented bacteria liquid (the bacterial classification is at logarithmic phase, the concentration is 1 × 10 8 CFU / ml);
[0053] 4. Obtaining the fermented puree of rice rose
[0054] 20mL of the fermented bacteria liquid t...
Embodiment 2
[0057] Application of rice rose fermented puree as cosmetics
[0058] 1. The properties of rice rose fermented puree cosmetics
[0059] After the rice rose fermented puree prepared in Example 1 is decolorized and deodorized by activated carbon, the rice rose fermented puree cosmetic is obtained. Its appearance is viscous liquid, viscosity 200-500cP, color is light white to light yellow, pH The value is 5.6-6.8, the soluble solid content is 3-4%, the total number of colonies is less than 50CFU / ml, no pathogenic bacteria are detected, and it meets the quality requirements of cosmetics.
[0060] The composition analysis of the rice rose fermented puree cosmetics is carried out. The detection method of protein refers to GB5009.5-2010; the detection method of crude polysaccharide refers to GB / T 5009.8-2008; the detection method of flavone refers to GB / T 5009.124-2003; The results of GB / T 8313-2008 are as follows:
[0061] The rice rose fermented puree cosmetic prepared in Example...
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