Method for inhibiting geosmin in yeast for making hard liquor
A kind of earthy element and Daqu technology, applied in the field of microorganisms, can solve the problems of inability to apply, affect the aroma and quality of liquor, destroy the micro-ecological structure, etc., and achieve the effect of obvious decline.
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[0033] Specific steps are as follows:
[0034] (1) Pick a single colony of Bacillus licheniformis CGMCC No: 3963, inoculate it in a medium, and culture it for 20 hours at 37°C and 220 r / min to obtain Bacillus licheniformis CGMCC No: 3963 broth; Centrifuge the Bacillus licheniformis CGMCC No: 3963 and resuspend it in a protective agent containing 100g / L skimmed milk powder to a bacterial concentration of 1×10 8 CFU / mL to obtain Bacillus licheniformis CGMCC No: 3963 bacterial suspension;
[0035] A single colony of Bacillus pumilus (Bacillus pumilus) CCTCC No: M2013372 was inoculated into a medium and cultured at 37°C and 220r / min for 20 hours to obtain the Bacillus pumilus CCTCC No: M2013372 broth; Bacillus pumilus CCTCC No: M2013372 After centrifugation, the bacterial liquid was resuspended in a protective agent containing 100g / L skimmed milk powder to a bacterial concentration of 1×10 8 CFU / mL to obtain Bacillus pumilus (Bacillus pumilus) CCTCC No: M2013372 bacterial suspension;
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