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Fluorescence in situ hybridization pretreatment liquid and pretreatment method

A technique of fluorescence in situ hybridization and pretreatment solution, which is applied in fluorescence/phosphorescence, preparation of test samples, material analysis by optical means, etc. High cost problems, to achieve the effect of shortening the operation time, avoiding the decline of dewaxing ability, and high signal-to-noise ratio

Inactive Publication Date: 2019-07-05
JIAXING ACCB DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These toxic and hazardous reagents not only pose hidden dangers to the safety of operators, but also have higher requirements for laboratory facilities, and the input costs for the subsequent treatment of these chemical wastes are higher.

Method used

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  • Fluorescence in situ hybridization pretreatment liquid and pretreatment method
  • Fluorescence in situ hybridization pretreatment liquid and pretreatment method
  • Fluorescence in situ hybridization pretreatment liquid and pretreatment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The formula of 1% Triton X-100 in embodiment 1 detects the result of non-small cell lung cancer sample FISH-ALK as follows figure 2 Shown (embodiment 2-4 " +++ " each group result is similar to it).

[0047] The formula of 1% Triton X-100 in embodiment 1 detects the result of breast cancer sample FISH-HER2 as follows image 3 Shown (embodiment 2-4 " +++ " each group result is similar to it).

[0048] 2. Conclusion

[0049] Depend on figure 2 and image 3 It can be seen that the fluorescent signal intensity obtained by the method of the present invention is high (orange green in the color photo), the signal points are clear, the background is dark, and the signal-to-noise ratio is good.

[0050] In addition, adopting the method of the present invention greatly saves experimental operation time, and the average treatment time is about 25 minutes, which is about 65-85 minutes, saving about 2 / 3 of the time.

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Abstract

The invention relates to a fluorescence in situ hybridization pretreatment liquid and a pretreatment method. The pretreatment liquid comprises a weak acid buffer, 1 to 5% of nonionic surface active detergent, and 10 to 30% of glycerol. Preferably, the weak acid buffer is a citrate solution and comprises trisodium citrate dihydrate and citric acid monohydrate. The pretreatment method comprises thestep that after the a paraffin-embedded tissue section is subjected to roasting treatment, the slice is treated with the pretreatment liquid provided by the invention. Seven steps of three-step xylenedewaxing, three-step ethanol gradient rehydration, pre-processing and the like in conventional operation are replaced by one-step treatment. According to the invention, under the premise of ensuringthe experimental effect, the pre-treatment time is shortened, and the operation process is simplified; and toxic reagents such as xylene are replaced by safe reagents, which is safe and environmental-friendly.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a pretreatment solution for fluorescence in situ hybridization of paraffin-embedded sections and a corresponding pretreatment method. Background technique [0002] Formalin-fixed paraffin-embedded (Formalin-fixed paraffin embedded, FFPE) section is the core material of pathology, it is the histological sample of postoperative or biopsy, fixed in formalin, dehydrated in alcohol, cleared in xylene, After being infiltrated with liquid paraffin, it is made into a paraffin block. After slicing the mass, stick it on a glass slide to obtain a paraffin-embedded section (hereinafter referred to as a section), which is widely used in molecular pathological detection techniques such as HE, immunohistochemistry, and fluorescence in situ hybridization. [0003] Fluorescence in situ hybridization (FISH) is a new technique developed in the 1980s that combines molecular biology and cyto...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N21/64
CPCG01N1/30G01N21/6402G01N21/6486
Inventor 丁凤薛璞王琦余佶彦王皎洁许佳波王芳
Owner JIAXING ACCB DIAGNOSTICS