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Method for preparing ethanol by semi-continuous fermentation of surface-immobilized yeast

An immobilized yeast, semi-continuous technology, applied in biochemical equipment and methods, methods based on microorganisms, immobilized on/in organic carriers, etc., can solve the problems of poor ethanol production and avoid easy staining Bacteria, high efficiency, good adsorption effect

Active Publication Date: 2019-07-09
江苏省生产力促进中心 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a surface-immobilized yeast semi-continuous fermentation technology to solve the problems in the prior art such as the poor ethanol production effect of immobilized yeast fermentation

Method used

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  • Method for preparing ethanol by semi-continuous fermentation of surface-immobilized yeast
  • Method for preparing ethanol by semi-continuous fermentation of surface-immobilized yeast
  • Method for preparing ethanol by semi-continuous fermentation of surface-immobilized yeast

Examples

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Effect test

Embodiment 1

[0041] Example 1 Using synthetic medium as fermentation raw material and adopting bamboo fiber immobilized yeast semi-continuous fermentation to produce fuel ethanol

[0042] First, fix cotton fibers (thickness 0.22 cm) cut to a side length of 2.5 cm on a polypropylene figure 1 Prepare the immobilization medium on the central axis of the spherical grid with a diameter of 4.5cm (the side length of the ball hole is 0.8cm); then put the immobilization medium into a 100L reactor with a filling amount of 2g / L middle. Then the cultured strains were added to the fermenter, and fixed at a flow rate of 80 L / min for 42 hours. Until the bacterial cell adsorption concentration is 200 million / ml. Then drain to waste. Then feed the fermentation medium (glucose concentration is 220g / L, fermentation broth pH4.2), the liquid loading volume is 75%, carry out circulation fermentation, wherein temperature is 34 ℃, flow rate is 800L / h. The concentration of free yeast in the solution dropped to...

Embodiment 2

[0043] Example 2 Using cassava liquefied mash medium as fermentation raw material, bamboo fiber immobilized yeast was used for semi-continuous fermentation to produce fuel ethanol.

[0044] First, fix the column fiber (thickness 0.2cm) cut into a diameter of 18cm in a spherical grid with a diameter of 25cm (the side length of the ball hole is 5cm) made of polyethylene as shown in the figure, and prepare the immobilization medium; then fix the Put the chemical medium into a 20-ton reactor with a height-to-diameter ratio of 2.5:1 with a filling amount of 2.2g / L. Then the cultured strains were added to the fermenter, and fixed at a flow rate of 20 tons m3 / h for 48 hours. Until the concentration of free bacteria in the solution drops by about 182 million cells / ml. Then prepare cassava liquefied mash: the ratio of solid to liquid is 1:2.4, add liquefaction enzyme (15U / g cassava dry weight) to liquefy at 85°C for 2 hours, press filter, and discharge the immobilized seed waste liqui...

Embodiment 3

[0045] Example 3 Using molasses medium as fermentation raw material to carry out semi-continuous fermentation of immobilized yeast to produce fuel ethanol

[0046]At first, carrier preparation and immobilization method (using total sugar 180g / l molasses as seed medium) are the same as in Example 2. After the fixation ends, the acidified molasses is diluted to a sugar concentration of 245g / L and pumped into the immobilized fermenter for Circular fermentation, wherein the temperature is 35°C, the pH of the fermentation broth is 4.0, and the flow rate is 10m3 / h. Stable during the third batch of fermentation, and the concentration of free bacteria is 0.85 billion / ml, and when the alcohol content reaches 10% (v / v), discharge 50% of the material to an empty tank for post-fermentation, and then feed to the original volume, and continue Draining to the empty tank previously filled with 40% broth, after filling the tank, the immobilized fermenter broth is pumped into the second empty t...

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Abstract

The invention discloses a method for preparing ethanol by semi-continuous fermentation of surface-immobilized yeast. The method includes fastening a fiber material into a spherical grid to serve as animmobilization medium, filling a fermentation tank with the immobilization medium, fastening the yeast to a fiber carrier through adsorption to carry out fermentation, completing the fermentation after 2-4 batches are completed, discharging materials into another fermentation tank when the alcohol content of each batch reaches 8-10% (v / v), supplementing the materials until the fermentation tank is full, and continuing to discharge the materials into another empty tank until the alcohol content reaches 8-10% (v / v); carrying out semi-continuous fermentation by repeating the previous steps. Themethod has the advantages of low production cost, convenience in operation, stability in process, high fermentation strength, high product yield, and long-lasting and high activity of the immobilizedyeast.

Description

technical field [0001] The invention belongs to the technical field of industrial biology, and in particular relates to a surface-immobilized yeast semi-continuous fermentation technology. Background technique [0002] Due to the overexploitation of fossil energy and other non-renewable energy sources, the energy crisis has become the primary problem to be solved in the development of the world today. Finding new alternative energy sources has become the focus of energy strategies over the years. Ethanol is not only a renewable fuel, but also can be mixed with gasoline in any proportion to ease the pressure on oil demand, and it has always been a good energy substitute. At present, fuel ethanol production enterprises usually adopt the method of large inoculation amount (20-35%) for free fermentation, the bacterial concentration is high (>250 million / ml), and the sugar consumption is high, resulting in a certain degree of waste; in addition, Due to mechanical agitation a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/12C12N11/08C12P7/06C12R1/865
CPCC12N11/12C12N11/08C12P7/06Y02E50/10
Inventor 黄留琴刘庆国赵南邹亚男朱杰刘桂文陈勇应汉杰
Owner 江苏省生产力促进中心
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