Preparation method of phloretin

A phloretin and gravimetric technology, applied in the field of phloretin preparation, can solve problems such as unfavorable operation, long time-consuming, many operations, etc., to reduce the pressure of three-waste treatment, high conversion rate and purity, mild conditions and easy control Effect

Active Publication Date: 2019-07-09
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method requires pressurization or heating in the process of preparing naringin dihydrochalcone, which is not conducive to operation; when naringin dihydrochalcone is heated and hydrolyzed under the action of dilute acid, the operation is more time-consuming and longer, and there is a large amount of Waste acid needs to consume a large amount of alkali to neutralize, thus producing a large amount of waste brine
[0009]The routes mentioned above have their own advantages, but each route also has shortcomings, including irresistible factors, side effects and toxicity are difficult to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Mix 1 part of naringin dihydrochalcone with 8 parts of acetic acid-sodium acetate buffer solution (pH=5.5), heat the resulting mixture to 55°C, and then add 65 parts of α-L Rhamnosidase and 65 parts of β-D glucosidase were reacted for 45 minutes, cooled to 4° C. for 2.5 hours, and suction filtered to obtain the target product phloretin with a yield of 87.6%.

Embodiment 2

[0020] Example 2: Mix 1 part of naringin dihydrochalcone with 10 parts of acetic acid-sodium acetate buffer solution (pH=5.5), heat the resulting mixture to 50°C, and then add 60 parts of α-L Rhamnosidase and 24 parts of β-D glucosidase were reacted for 30 minutes, cooled for 1.5 hours to 0°C, and filtered by suction to obtain the target product phloretin with a yield of 85.6%.

Embodiment 3

[0021] Example 3: Mix 1 part of naringin dihydrochalcone with 12 parts of acetic acid-sodium acetate buffer solution (pH=5.5), heat the resulting mixture to 60°C, and then add 54 parts of α-L Rhamnosidase and 44 parts of β-D glucosidase were reacted for 60 minutes, cooled to 8° C. for 4 hours, and filtered by suction to obtain the target product, phloretin, with a yield of 88.4%.

[0022] By adopting the preparation method of the embodiment of the present invention, the discharge of waste acid can be greatly reduced, the operation is simple, the conditions are mild and easy to control, and the conversion rate and purity are high.

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PUM

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Abstract

The invention discloses a preparation method of phloretin. The method includes the following steps that naringin dihydrochalcone is mixed with an acetic-acid and sodium-acetate buffer solution with the pH of 5.5, the obtained mixture is heated to 50-60 DEG C, glycosidase is added into the mixture, the glycosidase refers to a mixed enzyme of alpha-L rhamnosidase and beta-D glucosaccharase, reactionis performed for 30-60 minutes, and the target product phloretin is obtained after cooling and suction filtration. The naringin dihydrochalcone is used for preparing phloretin under the catalysis ofglycosidase, discharge of waste acid can be largely reduced, operation is simple, conditions are mild and easy to control, the conversion rate and the purity are very high, finally a glycosidase solution as a reactant can be comprehensively recycled, and the pressure of three wastes treatment can thus be reduced.

Description

technical field [0001] The invention relates to a preparation method of phloretin. Background technique [0002] Phloretin, scientific name 2,4,6-trihydroxy-3-(4-hydroxyphenyl)propiophenone, belongs to dihydrochalcone compounds, easily soluble in methanol, ethanol and propanol, almost insoluble In water, soluble in alkaline solution. Phloretin is widely found in apples, pears and other fruits and various vegetable juices. It is named for its concentrated content in the root caps or root barks of these plants. It is a natural active substance and an anti-allergic agent in food. Mutation factor, and can be used in the treatment of skin cancer and other tumors, is a potential chemotherapy drug. [0003] Sy Jye LEU et al. isolated 7 kinds of phenolic substances from Taiwanese apples, including phloretin. The author also tested the activity of each substance. The experimental results showed that phloretin could significantly inhibit the activity of MMP-1 and elastase. Vitality...

Claims

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Application Information

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IPC IPC(8): C12P7/26
CPCC12P7/26Y02A50/30
Inventor 陆豫羌宇龚子杰张耀宗郑楠胡基志邬子彬余勃
Owner NANCHANG UNIV
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