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Lactobacillus reuteri and application thereof to production of selenium-enriched eggs

A technology of Lactobacillus reuteri and selenium-enriched Lactobacillus reuteri, which is applied in the field of lactic acid bacteria, can solve the problems that the safety of selenium-enriched eggs cannot be guaranteed, the content of selenium element is not high, the tolerance of poultry is low, and the like. The effect of improving quality and flavor, enriching nutrients, increasing selenium and protein content

Active Publication Date: 2019-07-16
SHANDONG UNIV +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the nutritional value of existing eggs is high, the content of selenium is not high
In the prior art, it is generally adopted to mix sodium selenite in the feed to feed laying hens to produce high-selenium eggs, but sodium selenite is inorganic selenium, which is highly toxic and poorly tolerated by poultry, which can easily cause hen poisoning and result in production of high-selenium eggs. Eggs fall, hens die, there is a big hidden danger
Therefore, the safety of selenium-enriched eggs obtained by simply feeding selenium elements cannot be guaranteed

Method used

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  • Lactobacillus reuteri and application thereof to production of selenium-enriched eggs
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  • Lactobacillus reuteri and application thereof to production of selenium-enriched eggs

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Experimental program
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Effect test

Embodiment 1

[0030] 16S rDNA analysis results of bacterial strains of the present invention

[0031] Extract the DNA of the bacterial strain using the bacterial genomic DNA extraction kit (spin column type); add 9.5 μL of ultrapure water, 12.5 μL of 2×PCR Master Mix, 1 μL of upstream and downstream primers, and 1 μL of DNA template into a sterile EP tube. After instant centrifugation, place in a fluorescent quantitative PCR instrument and amplify in the following PCR amplification conditions: 94°C pre-denaturation for 5 minutes, one cycle; 94°C denaturation for 1 minute, 65°C renaturation for 1 minute, 72°C extension for 20 seconds, and 30 cycles After that, continue to extend at 72°C for 10 minutes, one cycle; the amplified DNA was electrophoresed on a 1% agarose gel plate at 80V for 50 minutes, and then gel imaged; the DNA was sent to the sequence for comparison by BLAST method to identify the obtained Bacterial strains, the specific results are shown in Table 1;

[0032] Table 1 BLAST ...

Embodiment 2

[0036] Selenium tolerance performance test of Lactobacillus reuteri strain of the present invention

[0037] Pre-sterilized Na 2 SeO 3 Concentrated solution, made with Na 2 SeO 3 Screening media plates with different concentration gradients, Na in the plates 2 SeO 3The contents are respectively 10 μg / mL, 20 μg / mL, 30 μg / mL, 40 μg / mL, 50 μg / mL, 60 μg / mL, 70 μg / mL, 80 μg / mL, 90 μg / mL, 100 μg / mL; Spread Lactobacillus subtilis on the above-mentioned plate medium, incubate upside down at 37°C for 48 hours, observe the color change of plate colonies, determine the approximate concentration range of the strain’s resistance to selenium, and then divide the concentration range into finer Na 2 SeO 3 Concentration, repeat the above screening process, determine the concentration of bacterial strains resistant to selenium, the specific test results are shown in Table 2;

[0038] Table 2 Na 2 SeO 3 Selenium resistance test results of bacterial strains in the plate (unit: μg / mL)

...

Embodiment 3

[0043] Determination of the selenium-rich condition of Lactobacillus reuteri of the present invention

[0044] 1. Drawing of standard curve of selenium content

[0045] The selenium content was determined by the 3,3'-diaminobenzidine colorimetric method, as follows; 0 mL, 2 mL, 4 mL, 6 mL, 8 mL and 10 mL of sodium selenite standard solution with a concentration of 1 μg / mL were transferred into 125 mL Separating funnel, add water to 35mL, add 1mL of 5% DETA-2Na solution, shake well, adjust the pH value to 2~3, add 4mL of 0.5% 3,3'-diaminobenzidine solution, shake well, place in dark half an hour; adjust the pH value to neutral, add 10mL toluene and shake for 2min, let stand for stratification, discard the water layer, filter the toluene layer through a cotton plug into a cuvette, measure the absorbance at a wavelength of 420nm, and draw a standard curve , Se standard curve see figure 1 ;

[0046] 2. Determination of residual inorganic selenium content

[0047] Fermentation ...

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Abstract

The invention discloses lactobacillus reuteri and an application thereof to production of selenium-enriched eggs. The lactobacillus reuteri is characterized in that the biological preservation information is CGMCC No.16721. A safe reliable high-yield low-cost selenium-enriched egg production way is provided. The selenium-enriched eggs obtained by the lactobacillus reuteri cannot produce toxic andside effects on laying hens and human bodies, resistibility, immunity and production properties of the laying hens can also be improved, and economic benefits are increased.

Description

technical field [0001] The invention relates to a lactic acid bacterium, in particular to a Lactobacillus reuteri and its application in the production of selenium-enriched eggs. Background technique [0002] Selenium is one of the essential trace elements for the human body, which is closely related to the maintenance of normal physiological functions of the body and the occurrence and development of diseases. An appropriate amount of selenium can remove excessive active oxygen free radicals in the body, protect the structure and function of cell membranes from excessive oxidative damage, thereby delaying human aging and preventing tumors and cardiovascular diseases. In addition, selenium can stimulate the production of human immunoglobulin and antibodies, and enhance human immunity. Selenium deficiency in the human body can cause endemic diseases such as Keshan disease and Kaschin-Beck disease, and also increase the incidence of diseases such as cancer, cardiovascular dis...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38A23K10/18A23K10/30A23K20/163C12R1/225
CPCC12N1/20C12N1/38A23K10/18A23K10/30A23K20/163C12R2001/225C12N1/205A23V2400/173
Inventor 涂强张友明边志龙潘登尹杰印遇龙王湘文武玉侠符国安
Owner SHANDONG UNIV
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