Reagent kit for detecting endometrial cancer susceptibility related genetic marker

An endometrial cancer, genetic marker technology, applied in the direction of microbial determination/examination, biochemical equipment and methods, etc., can solve problems such as early prediction and diagnosis of diseases, and achieve the effect of simple technology, specific detection and high cost performance

Inactive Publication Date: 2019-07-19
ZHEJIANG UNIV
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Problems solved by technology

So far, highly expressed POU5F1 protein has been detected in cancer cells of various tumors. Although the detection of the protein level cannot effectively predict and diagnose the disease in the early stage, it suggests that it is possible for us to detect the genetic background of the POU5F1 gene at the DNA level. Molecular markers for early prediction of tumors discovered

Method used

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  • Reagent kit for detecting endometrial cancer susceptibility related genetic marker
  • Reagent kit for detecting endometrial cancer susceptibility related genetic marker
  • Reagent kit for detecting endometrial cancer susceptibility related genetic marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Association analysis between detection of POU5F1 gene single nucleotide polymorphism and susceptibility to endometrial cancer

[0029] 1.1 Research object

[0030] The case selection time was from August 2010 to December 2012. From the cases with clear pathological diagnosis in the Obstetrics and Gynecology Hospital Affiliated to Zhejiang University School of Medicine, 272 cases of endometrial cancer were randomly selected, and 346 cases of normal physical examination women were selected at the same time Volunteers were used as normal controls, and the inclusion criteria for normal controls were no other gynecological tumors, no other solid cancers and no immune diseases. All cases and normal control individuals were Han residents. All subjects obtained consent in accordance with the requirements of the Ethics Committee of the Obstetrics and Gynecology Hospital Affiliated to Zhejiang University School of Medicine, and all research methods followed the approv...

Embodiment 2

[0047] Example 2. POU5F1 Gene Single Nucleotide Polymorphism and Endometrial Cancer Susceptibility Detection Kit

[0048] 1. Based on the analysis results of Example 1, we found that the base variation of 501T>G in SEQ ID NO.1 (rs3130932) is significantly related to endometrial cancer, so we designed a specific site for the POU5F1 gene Specific PCR upstream and downstream primers for detection of point (rs3130932) single nucleotide polymorphism, and finally designed a kit for detection of peripheral blood DNA templates of endometrial cancer susceptible population.

[0049] 2. Prepare a special kit (100tests). The specific reagent composition is shown in Table 3, as follows:

[0050] Table 3. Reagent composition of POU5F1 gene single nucleotide polymorphism site detection kit

[0051]

[0052]

[0053] 3. Genomic DNA extraction from peripheral blood cells: extract 1mL of peripheral blood from the subject, use the conventional phenol-chloroform extraction method, or a thi...

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Abstract

The invention discloses a reagent kit for detecting an endometrial cancer susceptibility related genetic marker, and belongs to the technical field of molecular biology and biological medicine. The nucleotide sequence of the genetic marker is as shown in SEQID NO.1, single nucleotide polymorphism that T is greater than G exists in the 501st of the nucleotide sequence, namely that the 501st basic group of the sequence comprises two situations of a basic group T and a basic group G. The reagent kit provided by the invention contains 2 pairs of specific PCR primers designed based on single nucleotide polymorphism of a POU5F1 gene, the single nucleotide polymorphism of the POU5F1 gene is closely related to susceptibility that people suffer from endometrial cancer, through a PCR technique, thesingle nucleotide polymorphism in the POU5F1 gene can be effectively detected, and further the susceptibility of the endometrial cancer is predicted, so that the reagent kit has important clinical significance in screening endometrial cancer susceptible population, detecting high-risk individuals, and performing early prediction, early diagnosis and individualized treatment of the endometrial cancer.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and biomedicine, in particular to a kit for detecting the genetic marker of the single nucleotide polymorphism of the endometrial cancer susceptibility-related POU5F1 gene. Background technique [0002] Endometrial cancer is one of the most common gynecological tumors in the world. Its incidence has been increasing over the past few decades and tends to be younger, especially in developed countries. According to the FIGO 2009 classification, 80% of endometrial cancers are clinical stage I-II when diagnosed, the 5-year survival rate of stage I is 89.6%, that of stage II drops to 78.3%, that of stage III is 61.9%, and that of stage IV is 21.1%. Although endometrial cancer diagnosed early can be cured with surgery plus adjuvant radiotherapy or chemotherapy, approximately 15% of patients will relapse and progress. [0003] The etiology of endometrial cancer remains unclear due to the comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/156
Inventor 叶枫陈怀增王浛知洪蝶刘佳程琪周彩云
Owner ZHEJIANG UNIV
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