Functional sericin hydrogel and its preparation method and application
A sericin and hydrogel technology, applied in the field of tissue engineering, can solve the problems of poor elasticity, easy breakage, low transparency, etc., and achieve the effects of excellent affinity, simple preparation process, and wide range of gelation time.
Active Publication Date: 2022-04-15
JIANGSU UNIV OF SCI & TECH
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Problems solved by technology
In addition, the self-assembled sericin hydrogel we developed earlier, however, is not transparent, has poor elasticity and is easily broken
Method used
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Embodiment 1
[0041] A preparation method of sericin hydrogel, comprising the following steps:
[0042] 1. Silkworm cocoon selection
[0043]Silkworm silkworm cocoons (preserved by the Institute of Sericulture, Chinese Academy of Agricultural Sciences and provided commercially) are selected as raw materials, and the main chemical component is silkworm sericin.
[0044] 2. Extraction and separation of sericin
[0045] (1) Carry out impurity-removing treatment to the cocoons of silkworm mutant varieties, and cut into 0.2cm 2 fragments, take 1g of the shredded sericin cocoons for subsequent use;
[0046] (2) Mix the cocoon fragments obtained in step (1) with 50 mL of LiBr solution with a molar concentration of 6 mol / L, and place the mixed solution in a constant temperature water bath at 35° C. for 24 hours to dissolve sericin;
[0047] (3) Transfer the solution obtained in step (2) into a centrifuge tube and centrifuge at 5000 rpm for 10 minutes to remove insoluble substances and obtain a c...
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The invention discloses a functional sericin hydrogel and its preparation method and application. The method adopts silkworm cocoons of a specific silkworm silk fibroin-deficient mutant variety, extracts a sericin aqueous solution, and undergoes ultrasonic treatment to obtain silk with new characteristics. Colloidal hydrogel. The sericin hydrogel has high transparency; it has a microstructure different from traditional hydrogels, and the micropores of the hydrogel are arranged in a wavy order from large to small and from small to large; at the same time, it has excellent The characteristics of elasticity, injectability and controllable gelation time; the sericin hydrogel also has certain application value in drug controlled release; the sericin hydrogel has a strong affinity for cells and can support the adhesion and proliferation of cells; sericin hydrogel and its three-dimensional porous sericin bioscaffold obtained by freeze-drying can be used as extracellular matrix to support cell growth and promote nutrient exchange, and can be applied to many repair of tissue damage and treatment of disease.
Description
technical field [0001] The invention belongs to the field of tissue engineering and relates to a biomedical material, specifically functional sericin hydrogel and its preparation method and application. Background technique [0002] Hydrogel is a network structure formed by a variety of intermolecular forces, including ionic bonds, hydrogen bonds, hydrophobic interactions or covalent bonds, which can accommodate a large amount of water and are easily soluble in water. Hydrogel has pores, can support water and material exchange, and its physical and chemical properties are similar to natural tissue scaffolds. As an important class of biomaterials, hydrogels have a wide range of applications in the biomedicine and skin care industries. [0003] Among the many materials for preparing hydrogels, natural biomaterials are favored because of their characteristics of cell signal recognition, promotion of cell proliferation and differentiation, and good biocompatibility. [0004] ...
Claims
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IPC IPC(8): C08J3/075C08J3/28C08L89/04A61L27/22A61L27/52A61L27/56A61K8/64A61K8/04A61K9/06A61K47/42
CPCC08J3/075C08J3/28A61L27/227A61L27/52A61L27/56A61K8/64A61K8/042A61K9/06A61K47/42C08J2389/04C08L89/00
Inventor 杨洁陆莹霞江茹兰张业顺张国政张议文方梦莹凌敏史紫凌李聪
Owner JIANGSU UNIV OF SCI & TECH