Microfluidic chip and exosome extraction method

Abstract

The invention discloses a microfluidic chip and an exosome extraction method. The microfluidic chip comprises a substrate and a microfluidic channel layer on the substrate, wherein the microfluidic channel layer is provided with a sample inlet, a sample outlet, and a plurality of mixed channels and a magnetic chamber communicated between the sample inlet and the sample outlet, and the magnetic chamber is provided with a magnetic disk. According to the invention, exosomes can be continuously, automatically and efficiently purified, the sample dosage requirement is less, the chip size is less, and the microfluidic chip is convenient to carry. by using the microfluidic chip to extract the exosomes, the degree of automation is high, the separation time is short and the efficiency is high; andin the extraction process, physical damages caused to the exosomes are greatly reduced.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a microfluidic chip and an exosome extraction method. Background technique [0002] Exosomes are nanoscale extracellular vesicles with a diameter of 30nm-150nm, which can be secreted by cells cultured in vivo or in vitro, and exist in human body fluids such as urine, serum, plasma, and milk. In recent years, exosomes have been considered as important biomarkers for emerging clinical diagnosis and treatment, which can reveal the physiological information of cells and provide new methods for clinical diagnosis. [0003] Existing exosome isolation methods include ultracentrifugation, sucrose density gradient centrifugation, commercial kit precipitation, polymer precipitation, and immunoaffinity. However, these traditional exosome extraction methods have some problems, such as cumbersome operation process, low extraction purity and expensive extraction equipment. Among them, ...

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Problems solved by technology

However, these traditional exosome extraction methods have some problems, such as cumbersome operation process, low extraction purity and expensive extraction equipment, etc.

Among them, the ultracentrifugation method has high requirements on equipment, and the amount of sample consumed in the experiment is large. At the same time, the experimental operation time is very long and the purity is low; the precipitation method is easily contaminated by other co-precipitated substances, which affects the later experimental analysis; the immunoaffinity method cannot be batched. Isolate exosomes

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