Tumor exosome nanometer fluorescence sensor based on magnetic separation and DNA self-assembling

A technology of antibodies and magnetic beads, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of large sample size, cumbersome detection methods, unsuitable for clinical detection, etc., achieve wide detection range, realize rapid clinical detection, and high sensitivity high effect

Active Publication Date: 2019-08-06
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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Problems solved by technology

[0005] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a tumor exosome nano-fluorescence sensor based on magnetic separation and DNA self-assembly, which is used to solve the cumbersome operation of the exosome detection method in the prior art and the A large sample size is required, and it is not suitable for clinical testing, etc.

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  • Tumor exosome nanometer fluorescence sensor based on magnetic separation and DNA self-assembling
  • Tumor exosome nanometer fluorescence sensor based on magnetic separation and DNA self-assembling
  • Tumor exosome nanometer fluorescence sensor based on magnetic separation and DNA self-assembling

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Embodiment 1

[0052] 1. Principles analysis

[0053] (1) Separation method

[0054] Immune -pro -legal is to use the specific interaction between antibodies and exosoma gog protein to achieve specific capture of the exogenous body. Antibodies are usually fixed on the matrix of magnetic particles, chromat fixing phase, and microfluidal device. The most commonly used immune magnetic bead method is a combination of spherical magnetic nanoparticles with single cloning antibodies as a reaction interface with an exogeneic subtype of an exogenous body that expresses specific proteins. The body is separated. Because the separation process is simple and fast, the immune magnetic bead method is particularly suitable for the exogenous separation of the sample volume and the rapid detection of subsequent biosensions. And due to the liquid phase (solution-pHASE) reaction of the immune-immocoid bead as the reaction interface, the response area is large, the contact area of ​​material is more comprehensive an...

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Abstract

The invention provides a tumor exosome nanometer fluorescence sensor based on magnetic separation and DNA self-assembling. The sensor comprises a functionalized magnetic bead coupled with a GPC-1 antibody, a trigger chain, and three stem circular nucleic acid probes. According to the invention, quantitative detection of the GPC-1(+) exosome subgroup of the breast cancer is realized; and the detection specificity and sensitivity are high and the detection range is wide. The clinical application value of the method is evaluated by detecting the breast cancer GPC-1(+) exosome subgroup in the clinical plasma source specimen. The method having high universality not only can be applied to other biomarkers with specific aptamers screened out but also can be applied to detection of other exosome subgroups, proteins, cells and metal ions and the like.

Description

Technical field [0001] The present invention involves the field of tumor liquid detection technology, especially a tumor exogenous nano fluorescence sensor based on magnetic separation and DNA self -assembly. Background technique [0002] Breast cancer is the world's most common female malignant tumor. China Cancer statistics in 2015 showed that in the past ten years, Chinese breast cancer patients accounted for 15 % of all female cancer patients, and the mortality rate has increased significantly. It is the most common cause of cancer for women under 45. Early discovery and early diagnosis are important means to improve the prognosis of patients with breast cancer and increase the survival rate. At present, early screening measures for breast cancer include: breast X-ray, breast ultrasound, mammary MRI, clinical physical examination and self-examination; diagnostic measures include: cytology, pathological biopsy, and positive electronic launch computer tomography (PET-CT). The a...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/574G01N33/543G01N33/58
CPCG01N33/577G01N33/57492G01N33/57415G01N33/54326G01N33/54346G01N33/582G01N2333/70596
Inventor 郑磊李博刘春辰潘炜伦司徒博安泰学张晔张涵
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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